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The Importance of Retromobilization to Gene Dissemination and the Effect of Heavy Metal Pollution on Retromobilization in Soil

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Gene Transfers and Environment

Abstract

A phenomenon that warrants attention in the context of gene dissemination in the environment is retromobilization. Retromobilization (or retrotransfer) is the ability of some conjugative plasmids (in particular IncP1 plasmids) to mobilize genes in the opposite direction (from recipient to donor of the conjugative plasmid). This phenomenon has been observed for chromosomal markers, non-mobilisable and mobilisable vectors (Mergeay et al. 1987; Mergeay et al. 1990; Top et al. 1990). As retromobilization appears to be a way for bacteria to capture new genes from other species, an enhanced spread of introduced genetic material into a microbial community could be expected when retromobilizing plasmids are present among the indigenous microbiota.

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References

  • Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36:525–557

    PubMed  CAS  Google Scholar 

  • De Rore H, Top E, Mergeay M, Verstraete W (1992) The effect of heavy metal pollution on the dissemination of heavy metal resistance genes in soil microcosms Submitted to Appl Environ Microbiol

    Google Scholar 

  • Freter R, Freter RR, Brickner H (1983) Experimental and mathematical models of Escherichia coli plasmid transfer in vivo and in vitro. Infect Immun 39:60–84.

    PubMed  CAS  Google Scholar 

  • Levin BR, Stewart FM, Rice VA (1979) The kinetics of conjugative plasmid transmission: fit of a simple mass action model. Plasmid 2:247–260.

    Article  PubMed  CAS  Google Scholar 

  • Mergeay M, Lejeune P, Sadouk A, Gerits J, Fabry L (1987) Shuttle transfer (or retrotransfer) of chromosomal markers mediated by plasmid pULBl 13. Mol Gen Genet 209:61–70.

    Article  PubMed  CAS  Google Scholar 

  • Mergeay M, Springael D, Top E (1990) Gene transfer in polluted soils. In J.C. Fry and M.C. Day (eds), Bacterial genetics in natural environments. Chapman & Hall, London, New York, p. 152–171.

    Chapter  Google Scholar 

  • Murray NE, Brammar WJ, Murray K (1977) Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet 150: 53–61

    Article  PubMed  CAS  Google Scholar 

  • Top E, Mergeay M, Springael D, Verstraete W (1990) Gene escape model: transfer of heavy metal resistance genes from Escherichia coli toAlcaligenes eutrophus on agar plates and in soil samples. Appl Environ Microbiol 56:2471–2479.

    PubMed  CAS  Google Scholar 

  • Top E, van der Lelie D, Verstraete W, Mergeay M (1992a) Characterization of the retromobilization phenomenon by use of a mutant defective in conjugation with a PI-type donor (ConP−) In preparation.

    Google Scholar 

  • Top E, Vanrolleghem P, Mergeay M, Verstraete W (1992b) Characterization of the retrotransfer phenomenon using mechanistic mathematical modeling. Submitted to J Bacteriol

    Google Scholar 

  • Van der Lelie D, Top E, Nuyts G, Mergeay M (1992) Isolation of amutant defective in conjugation with a P-type donor (ConP−) that is lacking the major outer membrane protein OmpF. In preparation.

    Google Scholar 

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© 1992 Springer-Verlag Berlin Heidelberg

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Top, W., Vanrolleghem, P., De Rore, H., Van der Lelie, D., Mergeay, M., Verstraete, W. (1992). The Importance of Retromobilization to Gene Dissemination and the Effect of Heavy Metal Pollution on Retromobilization in Soil. In: Gauthier, M.J. (eds) Gene Transfers and Environment. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77450-8_14

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  • DOI: https://doi.org/10.1007/978-3-642-77450-8_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77452-2

  • Online ISBN: 978-3-642-77450-8

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