Abstract
Highly sensitive immunoassays have proved to be valuable in the diagnosis of viral infections because viral antigens can be directly detected in clinical specimens by these assays. This technology was first widely used in the radioimmunoassay of hepatitis B surface antigen in serum (Ling and Overby 1972) and later applied to the detection of gastroenteritis viruses in stool (Halonen and Meurman 1982). Our group has also developed assays for the detection of seven respiratory viruses in nasopharyngeal secretions (Sarkkinen et al. 1981a-c), and these assays have now been successfully applied on a routine basis in our diagnostic laboratory. Immuno-assays are now widely used in the detection of antiviral antibodies and particularly in the assay of IgM antibodies.
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References
Ekins RP (1981) The “precision profile”: its use in RIA assessment and design. Ligand Q 4: 33–44
Gardner PS, McQuillin J (1968) Application of the immunofluorescent antibody technique in the rapid diagnosis of respiratory syncytial virus infection. Br Med J 3: 340–343
Gardner PS, McQuillin J (1974) Rapid virus diagnosis. Application of immunofluorescence. Butter-worths, London
Halonen P, Meurman O (1982) Radioimmunoassay in diagnostic virology. In: Howard C (ed) New developments in practical virology. Liss, New York, pp 83–124
Halonen P, Sarkkinen H, Arstila P, Hjertsson E, Torfason E (1980) Four-layer radioimmunoassay for detection of adenovirus in stool. J Clin Microbiol 11: 614–617
Leung CS, Meares CF (1977) Attachment of fluorescent metal chelates to macromolecules using “Afunctional” chelating agents. Biophys Biochem Res Commun 75: 149–155
Ling CM, Overby LR (1972) Prevalence of hepatitis B virus antigen revealed by direct radioimmune assay with 125I-antibody. J Immunol 109: 834–841
Meurman OH, Hemmilä IA, Lövgren TN, Halonen PE (to be published) Time-resolved fluoro-immunoassay, a new test for rubella serology. J Clin Microbiol
Sarkkinen H (1981) Human rotavirus antigen detection by enzyme-immunoassay using antisera against Nebraska calf diarrhoea virus. J Clin Pathol 34: 680–685
Sarkkinen HK, Halonen PE, Arstila PP (1979) Comparison of radioimmonoassay and electron microscopy for the detection of human rotavirus. J Med Virol 4: 255–260
Sarkkinen HK, Tuokko H, Halonen PE (1980) Comparison of enzyme immunoassay and radioimmunoassay for detection of human rotaviruses and adenoviruses from stool specimens. J Virol Methods 1: 331–341
Sarkkinen HK, Halonen PE, Arstila PP, Salmi AA (1981a) Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease. J Clin Microbiol 13: 258–265
Sarkkinen HK, Halonen PE, Salmi AA (1981b) Detection of influenza A virus by radioimmunoassay and enzyme-immunoassay from nasopharyngeal specimens. J Med Virol 7: 213–220
Sarkkinen HK, Halonen PE, Salmi AA (1981c) Type-specific detection of parainfluenza viruses by enzyme-immunoassay and radioimmunoassay in nasopharyngeal specimens of patients with acute respiratory disease. J Gen Virol 56: 49–57
Soini E, Hemmila I (1979) Fluoroimmunoassay: present status and key problems. Clin Chem 25: 353–361
Sundberg MW, Meares CF, Goodwin DA, Diamanti CI (1974) Selective binding of metal ions to macromolecules usingbifunctional analogs of EDTA. J Med Chem 17: 1304–1307
Uotila M, Ruoslahti E, Engvall E (1981) Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alfa-fetoprotein. J Immunol Methods 42: 11–15
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© 1983 Springer-Verlag Berlin Heidelberg
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Halonen, P., Meurman, O., Lövgren, T., Hemmilä, I., Soini, E. (1983). Detection of Viral Antigens by Time-Resolved Fluoroimmunoassay. In: Bachmann, P.A. (eds) New Developments in Diagnostic Virology. Current Topics in Microbiology and Immunology, vol 104. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68949-9_8
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DOI: https://doi.org/10.1007/978-3-642-68949-9_8
Publisher Name: Springer, Berlin, Heidelberg
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