Summary
The preincubation of test compound, bacterial tester strain, and S-9 mix or buffer before pouring a minimal-glucose agar plate enhanced the sensitivity of mutation test and increased the spectrum of mutagens detected. Addition of NADH and ATP in S-9 mix enhanced the mutagenicity of some compounds. Addition of norharman in the preincubation mixture made it possible to detect a marginal or weak mutagenicity of certain types of mutagens. Addition of riboflavin revealed the mutagenicity of azo compounds. Glycosidase was required to detect the mutagenicity of glycosides or natural products.
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References
Ames BN, McCann J, Yamasaki E (1975) Method for detecting carcinogens and mutagens with Salmonella/mammalian microsome mutagenicity test. Mutat Res 31:347–363
McCann J, Choi E, Yamasaki E, Ames BN (1975) Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci USA 72:5135–5139
Sugimura T, Sato S, Nagao M, Yahagi T, Matsushima T, Seino Y, Takeuchi M, Kawachi T (1976) Overlapping of carcinogens and mutagens. In: Magee PN, Takayama S, Sugimura T, Matsushima T (eds) Fundamentals in cancer prevention. University Park Press, Baltimore, pp 191–215
Purchase IFH, Longstaff E, Ashby J, Styles JA, Anderson D, Lefevre PA, Westwood FR (1978) An evaluation of 6 short-term tests for detecting organic chemical carcinogens. Br J Cancer 37:873–959
Ames BN, McCann J, Lee FD, Durston WE (1973) An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proc Natl Acad Sci USA 70:782–786
McCann J, Spingarn NE, Kobori J, Ames BN (1975) Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci USA 72:979–983
Ames BN, Durston WE, Yamasaki E, Lee FD (1973) Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci USA 70:2281–2285
Mailing HV (1974) Mutagenic activation of dimethylnitrosamine and diethylnitrosamine in the host-mediated assay and the microsomal system. Mutat Res 26:465–472
Gabridge MG, Legator MS (1969) A host-mediated assay for the detection of mutagenic compounds. Proc Soc Exp Biol Med 130:831–834
Yahagi T, Nagao M, Seino Y, Matsushima T, Sugimura T, Okada M (1977) Mutagenicities of N-nitrosamines on Salmonella. Mutat Res 48:121–130
Sugimura T, Yahagi T, Nagao M, Takeuchi M, Kawachi T, Hara K, Yamasaki E, Matsushima T, Hashimoto Y, Okada M (1976) Validity of mutagenicity testing using microbes as a rapid screening method for environmental carcinogens. In: Montesano R, Bartsch H, Tomatis L (eds) Screening tests in chemical carcinogenesis. IARC, Lyon, pp 81–101
Yamanaka H, Nagao M, Sugimura T, Furuya T, Shirai A, Matsushima T (1979) Mutagenicity of pyrolizidine alkaloids in the Salmonella/mammalian-microsome test. Mutat Res 68:211–216
Nagao M, Yahagi T, Honda M, Seino Y, Kawachi T, Sugimura T, Wakabayashi K, Tsuji K, Kosuge T (1977) Comutagenic actions of norharman derivatives with 4-dimethylaminoazobenzene and related compounds. Cancer Lett 3:339–346
Sugimura T, Nagao M, Kawachi T, Honda M, Yahagi T, Seino Y, Sato S, Matsukura N, Matsushima T, Shirai A, Sawamura M, Matsumoto H (1977) Mutagen-carcinogens in food, with special reference to highly mutagenic pyrolytic products in broiled foods. In: Hiatt HH, Watson JD, Winsten JA (eds) Origins of human cancer. Cold Spring Harbor Lab. Cold Spring Harbor, New York, pp 1561–1577
Sugimura T, Kawachi T, Nagao M, Yahagi T, Seino Y, Okamoto T, Shudo K, Kosuge T, Tsuji K, Wakabayashi K. Iitaka Y, Itai A (1977) Mutagenic principle(s) in tryptophan and phenylalanine pyrolysis products. Proc Jpn Acad 53B:58–61
Nagao M, Yahagi T, Kawachi T, Sugimura T, Kosuge T, Tsuji K, Wakabayashi K, Mizusaki S, Matsumoto T (1977) Comutagenic action of norharman and harman. Proc Jpn Acad 53B:95–98
Nagao M, Yahagi T, Honda M, Seino Y, Matsushima T, Sugimura T (1977) Demonstration of mutagenicity of aniline and o-toluidine by norharman. Proc Jpn Acad 53B:34–37
Sugimura T, Nagao M, Matsushima T, Yahagi T, Hayashi K (1977) Recent findings on the relation between mutagenicity and carcinogenicity. Nucleic Acids Res special publ no 3:541–544
Hayashi K, Nagao M, Sugimura T (1977) Interactions of norharman and harman with DNA. Nucleic Acids Res 4:3679–3685
Nagao M, Yahagi T, Sugimura T (1978) Differences in effects of norharman with various classes of chemical mutagens and amounts of S-9. Biochem Biophys Res Commun 83:373–378
Levitt RC, Legraverend C, Nebert DW, Pelkonen O (1977) Effects of harman and norharman on the mutagenicity and binding to DNA of benzo(a)pyrene metabolites in vitro and on aryl hydrocarbon hydroxylase induction in cell culture. Biochem Biophys Res Commun 79:1167–1175
Fujino T, Fujiki H, Nagao M, Yahagi T, Seino Y, Sugimura T (1978) The effect of norharman on the metabolism of benzo(a)pyrene by rat-liver microsomes in vitro in relation to its enhancement of the mutagenicity of benzo(a)pyrene. Mutat Res 58:151–158
Smith DWE (1966) Mutagenicity of cycasin aglycone (methylazoxymethanol), a naturally occurring carcinogen. Science 152:1273–1274
Matsushima T, Matsumoto H, Shirai A, Sawamura M, Sugimura T (1979) Mutagenicity of the naturally occurring carcinogen cycasin and synthetic methylazoxymethanol conjugates in Salmonella typhimurium. Cancer Res 39:3780–3782
Sugimura T, Nagao M, Matsushima T, Yahagi T, Seino Y, Shirai A, Sawamura M, Natori S, Yoshihira K, Fukuoka M, Kuroyanagi M (1977) Mutagenicity of flavone derivatives. Proc Jpn Acad 53B:194–197
Bjeldanes LF, Chang GW (1977) Mutagenic activity of quercetin and related compounds. Science 197:577–578
Brown JP, Dietrich PS, Brown RJ (1977) Frameshift mutagenicity of certain naturally occurring phenolic compounds in the ‘Salmonella/microsome’ test: activation of anthraquinone and flavonol glycosides by gut bacterial enzymes. Biochem Soc Trans 5:1489–1492
MacGregor JT, Jurd L (1978) Mutagenicity of plant flavonoids: structural requirements for mutagenic activity in Salmonella typhimurium. Mutat Res 54:297–309
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© 1980 Springer-Verlag Berlin Heidelberg
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Matsushima, T., Sugimura, T., Nagao, M., Yahagi, T., Shirai, A., Sawamura, M. (1980). Factors Modulating Mutagenicity in Microbial Tests. In: Norpoth, K.H., Garner, R.C. (eds) Short-Term Test Systems for Detecting Carcinogens. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67202-6_20
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DOI: https://doi.org/10.1007/978-3-642-67202-6_20
Publisher Name: Springer, Berlin, Heidelberg
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