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Gene Cloning with Small Plasmids

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Current Topics in Microbiology and Immunology

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 78))

Abstract

The following account of the use of plasmids in gene cloning is an attempt to present this technique against a background of its subsidiary techniques—plasmid genetics, DNA biochemistry, bacterial transformation etc. —while at the same time considering the ways in which the field is likely to develop in view of the particular aims of different categories of cloning experiments. Rapid progress in this area of research will require regular review articles to keep abreast of new developments. New material was incorporated in this review up to the end of January 1977.

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Abbreviations

Ap:

Ampicillin resistance

CAM:

Chloramphenicol

cDNA:

complementary DNA

Cb:

Carbenicillin resistance

Cm:

Chloramphenicol resistance

ds:

double stranded

ss:

single stranded

dNTP:

deoxyribonucleotide triphosphate

dA:

deoxyadenosine

dT:

deoxythymidine

dC:

deoxycytidine

dG:

deoxyguanosine

IS:

insertion sequence

Km:

Kanamycin resistance

Md:

million daltons

NTP:

ribonucleotide triphosphate

A:

adenosine

U:

uridine

C:

cytidine

G:

guanosine

Nm:

Neomycin resistance

Tc:

tetracycline resistance

TdT:

deoxyribonucleotidyl terminal transferase

tRNA:

transfer RNA

ts:

temperature sensitive

Tn:

transposon (TnA=ampicillin transposon) or transposition element

EcoRI:

restriction endonuclease EcoRI

X:

purine

Y:

pyrimidine

References

  • Adhya, S., Gottesman, M., De Crombrugghe, B.: Release of polarity in Escherichia coli by gene N of phage X: termination and antitermination of transcription. Proc. Natl. Acad. Sci. U.S.A. 71, 2534–2538 (1974)

    PubMed  CAS  Google Scholar 

  • Akagawa, H., Okanishi, M., Umezawa, H.: A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping. J. Gen. Microbiol. 90, 336–346 (1975)

    PubMed  CAS  Google Scholar 

  • Anagnostopoulus, C., Spizizen, J.: Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81, 741–746 (1961)

    Google Scholar 

  • Anderson, E.S.: The ecology of transferable drug resistance in Enterobacteria. Annu. Rev. Microbiol. 22, 131–180 (1968)

    PubMed  CAS  Google Scholar 

  • Anderson, J.N., Schimke, R.T.: Partial purification of the ovalbumin gene. Cell 7, 331–338 (1976)

    PubMed  CAS  Google Scholar 

  • Arber, W., Linn, S.: DNA modification and restriction. Annu. Rev. Biochem. 38, 467–500 (1969)

    PubMed  CAS  Google Scholar 

  • Arber, W.: DNA modification and restriction. Prog. Nucleic Acid Res. Mol. Biol. 14, 1–50 (1974)

    PubMed  CAS  Google Scholar 

  • Ausubel, F.M., Cannon, F.C., Riedel, R.: Cloning of his and nif genes from Klebsiella pneumoniae. Personal communication (1976)

    Google Scholar 

  • Avery, O.T., Meod, C.M., Marty, M.: Studies on the chemical nature of the substance inducing transformation of Pneumococcal types. J. Exp. Med. 79, 137–158 (1944)

    PubMed  CAS  Google Scholar 

  • Berg, D.E., Davies, J., Allet, B., Rochaix, J.-D.: Transposition of R factor genes to bacteriophage X. Proc. Natl. Acad. Sci. U.S.A. 72, 3628–3632 (1975)

    PubMed  CAS  Google Scholar 

  • Berg, P.E., Gay da, R., Avni, H., Zehnbauer, B., Markovitz, A.: Cloning of Escherichia coli DNA that controls cell division and capsular polysaccharide synthesis. Proc. Natl. Acad. Sci. U.S.A. 73, 697–701 (1976)

    PubMed  CAS  Google Scholar 

  • Bigger, C.H., Murray, K, Murray, N.E.: Recognition sequence of a restriction enzyme. Nature [New Biol.] 244, 7–10 (1973)

    CAS  Google Scholar 

  • Blair, D. G., Sherratt, D. J., Clewell, D. B., Helinski, D. R.: Isolation of supercoiled colicinogenic factor EI DNA sensitive to ribonuclease and alkali. Proc. Natl. Acad. Sci. U.S.A. 69, 2518–2522 (1972)

    PubMed  CAS  Google Scholar 

  • Blin, N., Gabain, A.V., Bujard, H.: Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett. 53, 84–86 (1975)

    PubMed  CAS  Google Scholar 

  • Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., Falkows, S.: Construction and characterisation of a new E. coli plasmid, pBR322, serving as a cloning vehicle in an EK2 system. Gene (1977) in press.

    Google Scholar 

  • Bollum, F. J.: Terminal deoxynucleotidyl transferase. In: The Enzymes. Boyer, P.D. (ed.). New York: Academic Press 1974, Vol. X, pp. 145–171

    Google Scholar 

  • Borst, P., Aaij, C.: Identification of the heavy strand of rat liver mitochondrial DNA as the messenger strand. Biophys. Res. Commun. 34, 358–364 (1969)

    CAS  Google Scholar 

  • Boyer, H.W., Chow, L.T., Dugaiczyk, A., Hedgpeth, J., Goodman, H.M.: DNA substrate site for the Ecll restriction endonuclease and modification methylase. Nature [New Biol.] 224, 40–43 (1973)

    Google Scholar 

  • Brammar, W.J., Murray, N.E., Winton, S.: Restriction of k trp bacteriophages by Escherichia coli K. J. Molec. Biol. 90, 633–647 (1974)

    PubMed  CAS  Google Scholar 

  • Brown, D.D., Stern, R.: Methods of gene isolation. Annu. Rev. Biochem. 43, 667–693 (1974)

    PubMed  CAS  Google Scholar 

  • Brown, W.M., Watson, R.M., Vinograd, J., Tait, K.M., Boyer, H.W., Goodman, H.W.: The structure and fidelity of replication of Mouse mitochondrial DNA-C101 Ecl recombinant plasmids grown in E. coli K12. Cell. 7, 517–530 (1976)

    PubMed  CAS  Google Scholar 

  • Bursztyn, H., Sgaramella, V., Ciferri, O., Lederberg, J.: Transfectability of rough strains of Salmonella typhimunium. J. Bacteriol. 124, 1630–1634 (1975)

    PubMed  CAS  Google Scholar 

  • Cabello, F., Timmis, K., Cohen, S.N.: Replication control in a somposite plasmid constructed by in vitro linkage of two distinct replicons. Nature (Lond.) 259, 285–290 (1976)

    CAS  Google Scholar 

  • Cameron, J. R., Panasenko, S. M., Lehman, L. R., Davis, R.W.: In vitro construction of bacteriophage k carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proc. Natl. Acad. Sci. U.S.A. 72, 3416–3420 (1975)

    PubMed  CAS  Google Scholar 

  • Cannon, F.C., Dixon, R.A., Postgate, J.R., Primrose, S.B.: Plasmids formed in nitrogen fixing Escherichia coli—Klebsiella pneumoniae hybrids. J. Gen. Microbiol. 80, 241–251 (1974)

    PubMed  CAS  Google Scholar 

  • Carbon, J., Clarke, L., Ilgen, C., Ratzkin, B.: The construction and use of hybrid plasmid gene banks in Escherichia coli. In: Tenth Miles Symposium Cambridge, Mass., June 8–10, 1976 (in Press)

    Google Scholar 

  • Cavalli-Sforza, L., Lederberg, J.: Isolation of pre-adaptive mutants in bacteria by sib selection. Genetics 41, 367–381 (1956)

    PubMed  CAS  Google Scholar 

  • Chakrabarty, A.M., Mylroie, J.R., Friello, D.A., Vacca, J.G.: Transformation of Pseudomonas putida and Escherichia coli with plasmid-linked drug-resistance factor DNA. Proc. Natl. Acad. Sci. U.S.A. 72, 3647–3651 (1975)

    PubMed  CAS  Google Scholar 

  • Chakrabarty, A.M.: Molecular cloning in Pseudomonas. In: Microbiology American Society for Microbiology (Washington) 1976 (in press)

    Google Scholar 

  • Chandler, M. G., Pritchard, R. H.: The effect gene concentration and relative gene dosage on gene output in Escherichia coli. Molec. Gen. Genet. 138, 127–141 (1975)

    PubMed  CAS  Google Scholar 

  • Chang, A.C.Y., Cohen, S.N.: Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 71, 1030–1034 (1974)

    PubMed  CAS  Google Scholar 

  • Chang, A.C.Y., Lansman, R.A., Clayton, D.A., Cohen, S.N.: Studies of Mouse mitochondrial DNA in Escherichia coli: Structure and function of the eukaryotic-prokaryotic chimeric plasmids. Cell 6, 231–244 (1975)

    PubMed  CAS  Google Scholar 

  • Clarke, L., Carbon, J.: Biochemical construction and selection of hybrid plasmids containing specific segments of the Escherichia coli genome. Proc. Natl. Acad. Sci. U.S.A. 72, 4361–4365 (1975)

    PubMed  CAS  Google Scholar 

  • Clarke, L., Carbon, J.: A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9, 91–99 (1976)

    PubMed  CAS  Google Scholar 

  • Clewell, D.B., Helinski, D.R.: Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochem. 9, 4428–4440 (1970)

    CAS  Google Scholar 

  • Clewell, D.B.: Nature of Col plasmid replication in Escherichia coli in the presence of chloramphenicol. J. Bacteriol. 110, 667–673 (1972)

    PubMed  CAS  Google Scholar 

  • Clewell, D.B., Evenchik, B., Cranston, J.W.: Direct inhibition of Col El plasmid replication in Escherichia coli by rifampicin. Nature [New Biol.] 237, 29–31 (1972)

    CAS  Google Scholar 

  • Clowes, R.C.: Molecular structure of bacterial plasmids. Bacteriol. Rev. 36, 361–405 (1972)

    PubMed  CAS  Google Scholar 

  • Cohen, S. N., Chang, A. C. Y., Hsu, L.: Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 2110–2114 (1972)

    PubMed  CAS  Google Scholar 

  • Cohen, S.N., Chang, A.C.Y.: Recircularisation and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc. Natl. Acad. Sci. U.S.A. 70, 1293–1297 (1973)

    PubMed  CAS  Google Scholar 

  • Cohen, S.N., Chang, A.C.Y., Boy er, H.W., Helling, R.B.: Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. U.S.A. 70, 3240–3244 (1973)

    PubMed  CAS  Google Scholar 

  • Cohn, R.H., Lowry, J.C., Kedes, L.H.: Histone genes of the Sea Urchin (S. purpuratus) cloned in E. coli: order, polarity and strandedness of the five histone-coding and spacer regions. Cell 9, 147–161 (1976)

    PubMed  CAS  Google Scholar 

  • Collins, J., Lincoln, D. N.: A computer program to give potential endonuclease sites within genes for proteins of known amino-acid sequence, (unpublished; available on request from author)

    Google Scholar 

  • Collins, J., Pritchard, R.H.: Relationship between chromosome replication and F ’lac epi-some replication in Escherichia coli. J. Molec. Biol. 78, 143–155 (1973)

    PubMed  CAS  Google Scholar 

  • Collins, J., Johnsen, M., Jorgensen, P., Valentin-Hansen, P., Karlström, H. O., Gautier, F., Lindenmaier, W., Mayer, H., Sjörherg, B.M.: Expression of plasmid genes: Col and derivatives. American Society for Microbiology; Symposium on Extrachromosomal genetic elements. Jackson Hole (Ed. Novik, R, Davies, J.) (in press 1977)

    Google Scholar 

  • Collins, J., Jorgensen, P., Karlström, H. O., Lindenmaier, W., Johnsen, M., Sjöberg, B.-M.: Cloning of Escherichia coli genes important in the regulation of ribosome synthesis. In: Gene Insertions. New York: Cold Spring Harbor Publications 1976 (in press)

    Google Scholar 

  • Collins, J., Fiil, N.P., Jorgensen, P., Friesen, J.D.: Cloning of genes involved in regulation of ribosome synthesis in Escherichia coli. In: Control of Ribosome Synthesis. IX. Alfred Benzon Symposium, 1975, pp. 356–367. Copenhagen: Munksgaard Press 1976

    Google Scholar 

  • Collins, J., Yanofsky, S., Helinski, D. R.: Involvement of the DNA-protein relaxation complex in the replication of Col. Molec. Gen. Genet, (in press 1977)

    Google Scholar 

  • Cooper, S., Helmstetter, C. E.: Chromosome replication and the division cycle of Escherichia coli B/r. J. Molec. Biol. 31, 519–540 (1968)

    PubMed  CAS  Google Scholar 

  • Cosloy, S.D., Oishi, M.: Genetic transformation in Escherichia coli Kl2. Proc. Natl. Acad. Sci. USA 70, 84–87 (1973)

    PubMed  CAS  Google Scholar 

  • Covey, C., Richardson, D., Carbon, J.: A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid. Molec. Gen. Genet. 145, 155–158 (1976)

    PubMed  CAS  Google Scholar 

  • Curtiss, R. III: Bacterial conjugation. Annu. Rev. Microbiol. 23, 69–136 (1969)

    PubMed  CAS  Google Scholar 

  • Curtiss, R. III: Genetic manipulation of microorganisms: Potential benefits and biohasards. Annu. Rev. Microbiol. 30, 507–533 (1976)

    PubMed  CAS  Google Scholar 

  • Danna, K.J., Sack, G.H., Nathans, D.: Studies of simian virus 40 DNA, VII. A cleavage map of the SV40 genome. J. Molec. Biol. 78, 363–376 (1973)

    PubMed  CAS  Google Scholar 

  • Dawid, I. B.: Mitochondrial RNA in Xenopus laevis. I. The expression of the mitochondrial genome. J. Molec. Biol. 63, 201–216 (1972a)

    CAS  Google Scholar 

  • Dawid, I. B.: The evolution of mitochondrial DNA sequences in Xenopus. Dev. Biol. 29, 139 (1972b)

    PubMed  CAS  Google Scholar 

  • De Witt, W., Helinski, D.R.: Characterisation of colicinogenic factor El from a non-induced and a mitomycin C-induced Proteus strain. J. Molec. Biol. 13, 692–703 (1965)

    Google Scholar 

  • Dixon, R., Cannon, F., Kondor osi, A.: Construction of a P plasmid carrying nitrogen-fixation genes from Klebsiella pneumoniae. Nature (Lond.) 260, 268–271 (1976)

    CAS  Google Scholar 

  • Dugaiczyk, A., Boyer, H.W., Goodman, H.M.: Ligation of Ecl endonuclease-generated DNA fragments into linear and circular structures. J. Molec. Biol. 96, 171–184 (1975)

    PubMed  CAS  Google Scholar 

  • Dugaiczyk, A., Turner, E. G., Nasser, D., Boyer, H. W., Marthy, B. J., Goodman, H. M.: Cloning of Drosophila melanogaster Ecl DNA fragments. In: ICN-UCLA Symposium on Molecular and Cellular Biology. Vol. V. Nierlich, D. P., Rutter, W. J., Fox, C. F. (eds.). New York: Academic Press 1976

    Google Scholar 

  • Efstratiadis, A., Kafatos, F.C., Maxam, A.M., Maniatis, T.: Enzymatic in vitro synthesis of Globin genes. Cell 7, 279–288 (1976)

    PubMed  CAS  Google Scholar 

  • Engberg, B., Nordstrom, K.: Replication of the R-factor R1 in Escherichia coli K12 at different growth rates. J. Bacteriol. 725, 179–186 (1975)

    Google Scholar 

  • Engberg, J., Andersson, P., Vagn Leick, Collins, J.: Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes. J. molec. Biol. 104, 455–470 (1976)

    PubMed  CAS  Google Scholar 

  • Falkow, S., Haapala, D.K., Silver, R.P.: Relationships between extrachromosomal elements. CIBA Symposium: Bacterial Episomes and Plasmids, pp. 136–158. Wolstenholme, J., O’Connor, A. (eds). London: Churchill 1969

    Google Scholar 

  • Fareed, G. C., Wilt, E. M., Richardson, C. C.: Enzymatic breakage and joining of deoxyribonucleic acid. iii. Hybrids of ribo and deoxyribonucleotide homopolymers as substrate for polynucleotide ligase of bacteriophage T4. J. Biol. Chem. 246, 925–932 (1971)

    PubMed  CAS  Google Scholar 

  • Feix, G., Sano, H., Hobom, G.: Integration of a poly (. poly ()) segment into A DNA. Hoppe Seylers Z. Physiol. Chem. 357, 313–314 (1976)

    Google Scholar 

  • Firtel, R.A., Cockburn, A., Frankel, G., Hershfield, V.: Structural organisation of the genome of Dictyostelium discoideum: Analysis by Ecl restriction endonuclease. J. Molec. Biol. 102, 831–852 (1976)

    PubMed  CAS  Google Scholar 

  • Foster, T.J., Howe, T.G.B., Richmond, K.M.V.: Translocation of the tetracycline resistance determinant from R100-I to the Escherichia coli K12 chromosome. J. Bacteriol. 124, 1153–1158 (1975)

    PubMed  CAS  Google Scholar 

  • Frame, R., Bishop, J.O.: The number of sex factors per chromosome in E. coli. Biochem. J. 121, 93–103 (1971)

    PubMed  CAS  Google Scholar 

  • Frazer, A.C., Curtiss, R. III: Production, properties and utility of bacterial minicells. In: Current Topics in Microbiology and Immunology 69, pp. 1–84. Berlin: Springer-Verlag 1975

    Google Scholar 

  • Ganem, D., Nussbaum, A.L., Davoli, D., Fareed, G.C.: Propagation of a segment of bacteriophage A-DNA in Monkey cells after covalent linkage to a defective Simian virus 40 genome. Cell 7, 349–359 (1976)

    PubMed  CAS  Google Scholar 

  • Garfin, D.E., Boyer, H.W., Goodman, H.W.: Sequences spanning the Ecl substrate site. Nucleic Acids Res. 2, 1851–1865 (1975)

    PubMed  CAS  Google Scholar 

  • Gasson, M.J., Willetts, N.S.: Five control systems preventing transfer of Escherichia coli K12 sex factor F. J. Bacteriol. 122, 518–525 (1975)

    PubMed  CAS  Google Scholar 

  • Gautier, F., Mayer, H., Goebel, W.: Cloning of Calf thymus satellite I DNA in Escherichia coli. Molec. Gen. Genet. 149, 23–31 (1976)

    PubMed  CAS  Google Scholar 

  • Gilham, P. T.: Immobilized polynucleotides and nucleic acids. In: Advances in Experimental Medicine and Biology 42, pp. 173–185, Dunlap, R.B. (ed.). New York: Plenum Press 1974

    Google Scholar 

  • Glover, D.M., White, R.L., Finnegan, D.J., Hogness, D.S.: Characterisation of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for NA. Cell 5, 149–157 (1975)

    PubMed  CAS  Google Scholar 

  • Glover, D. M.: The construction and cloning of hybrid DNA molecules. In: New Techniques in Biophysics and Cell Biology. Vol. III. Pain. Smith (eds.). 1976 (in press)

    Google Scholar 

  • Goebel, W.: Replication of DNA of colicinogenic factor EI (Col) at restrictive temperature in a DNA replication mutant thermosensitive for DNA-polymerase III. Nature [New Biol] 237, 192 (1972)

    Google Scholar 

  • Goebel, W., Bonewald, R.: Class of small multicopy plasmids originating from the mutant antibiotic resistance factor Rldrd-19B2. J. Bacteriol. 123, 658–665 (1975)

    PubMed  CAS  Google Scholar 

  • Gottesman, M.M., Rosner, J.L.: Acquisition of a determinant for chloramphenicol resistance by coliphage 1 Proc. Natl. Acad. Sci. U.S.A. 72, 5041–5045 (1975)

    CAS  Google Scholar 

  • Greene, P.J., Betlach, M.C., Boyer, H.W., Goodman, H.M.: The Ecl restriction endonuclease. In: Methods in Molecular Biology, Vol. VII, DNA replication, pp. 87–111. Wickner, R.B. (ed.). New York: Marcell Dekker 1975

    Google Scholar 

  • Grindley, N.D.F., Kelley, W.S.: Effects of different alleles of the E. colAl po gene on the replication of non-transferring plasmids. Molec. Gen. Genet. 143, 311–318 (1976)

    PubMed  CAS  Google Scholar 

  • Gross, K., Probst, E., Schaffner, W., Bimstie I, M.: Molecular analysis of the histone gene cluster of Psammechinus miliaris. I. Fractionation and identification of five individual histone NAs. Cell 8, 455–469 (1976a)

    PubMed  CAS  Google Scholar 

  • Gross, K., Schaffner, W., Telford, J., Birnstiel, M.: Molecular analysis of the histone gene cluster of Psammechinus miliaris. III. Polarity and asymmetry of the histone-coding sequences. Cell 8, 479–484 (1976 b)

    PubMed  CAS  Google Scholar 

  • Grunstein, M., Hogness, D.S.: Colony hybridisation: A method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. U.S.A. 72, 3961–3965 (1975)

    PubMed  CAS  Google Scholar 

  • Guild, W.R., Robison, M.: Evidence for message reading from a unique strand of pneumococcal DNA. Proc. Natl. Acad. Sci. U.S.A. 50, 106–112 (1963)

    PubMed  CAS  Google Scholar 

  • Haggerty, D.M., Schleif, R.E.: Location in bacteriophage Lambda DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H. J. Virol. 18, 659–663 (1976)

    PubMed  CAS  Google Scholar 

  • Hamer, D.H., Thomas, Jr., C.A.: Molecular cloning of DNA fragments produced by restriction endonucleases Sail and Baml. Proc. Natl. Acad. Sci. U.S.A. 73, 1537–1541 (1976)

    PubMed  CAS  Google Scholar 

  • Hayashi, M.: A DNA-RNA complex as an intermediate of in vitro genetic transcription. Proc. Natl. Acad. Sci. U.S.A. 54, 1736–1743 (1965)

    PubMed  CAS  Google Scholar 

  • Hedges, R.W., Jacob, A.E.: Transposition of ampicillin resistance from RP4 to other replicons. Molec. Gen. Genet. 132, 31–40 (1974)

    PubMed  CAS  Google Scholar 

  • Hedges, R.W., Cresswell, J.M., Jacob, A.E.: A non-transmissible variant of RP4 suitable as cloning vehicle for genetic engineering. FEBS Lett. 61, 186–188 (1976)

    PubMed  CAS  Google Scholar 

  • Hedgpeth, J., Goodman, H.M., Boyer, H.W.: DNA nucleotide sequence restricted by the RI endonuclease. Proc. Natl. Acad. Sci. U.S.A. 69, 3448–3452 (1972)

    PubMed  CAS  Google Scholar 

  • Heffron, F., Rubens, C., Falkow, S.: Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. Natl. Acad. Sci. U.S.A. 72, 3623–3627 (1975)

    PubMed  CAS  Google Scholar 

  • Helinski, D.R.: Plasmid determined resistance to antibiotics: molecular properties of R factors. Annu. Rev. Microbiol. 27, 437–469 (1973)

    PubMed  CAS  Google Scholar 

  • Helinski, D.R., Herschman, H.R.: Effect of Rec mutations on the activity of colicinogenic factors. J. Bacteriol. 94, 700–706 (1967)

    PubMed  CAS  Google Scholar 

  • Helinski, D.R., Clewell, D.B.: Circular DNA. Annu. Rev. Biochem. 40, 899–942 (1971)

    PubMed  CAS  Google Scholar 

  • Helling, R.B., Goodman, H.M., Boyer, H.W.: Analysis of endonuclease R.Ecl fragments of DNA from Lambdoid bacteriophages and other viruses by agarose gel electrophoresis. J. Virol. 14, 1235–1244 (1974)

    PubMed  CAS  Google Scholar 

  • Helmuth, R., Achtman, M.: Operon structure of DNA transfer cistrons on the F sex factor. Nature (Lond.) 257, 652–656 (1975)

    CAS  Google Scholar 

  • Hershfield, V., Boyer, H.W., Yanofsky, C., Lovett, M., Helinski, D.R.: Plasmid Col as a molecular vehicle for cloning and amplification of DNA. Proc. Natl. Acad. Sci. U.S.A. 71, 3455–3459 (1974)

    PubMed  CAS  Google Scholar 

  • Hershfield, V., Boyer, H.W., Chow, L., Helinski, D.R.: Characterisation of a Mini-Col plasmid. J. Bacteriol. 126, 447–453 (1976)

    PubMed  CAS  Google Scholar 

  • Heyneker, H.L., Shine, J., Goodman, H.W., Boyer, H.W., Rosenberg, J., Dickerson, R.E., Narang, S.A., Itakura, K., Lin, S.-Y., Riggs, A.D.: Synthetic lac operator DNA is functional in vivo. Nature (Lond.) 263, 748–752 (1976)

    CAS  Google Scholar 

  • Hickson, F.T., Roth, T.F., Helinski, D.R.: Circular DNA forms of a bacterial sex factor. Proc. Natl. Acad. Sci. U.S.A. 58, 1731–1738 (1967)

    PubMed  CAS  Google Scholar 

  • Hill, C., Holland, LB.: Genetic basis of colicin E susceptibility in Escherichia coli. I. Isolation and properties of refractory mutants and the prelimary mapping of their mutations. J. Bacteriol. 94, 677–686 (1967)

    PubMed  CAS  Google Scholar 

  • Hohn, B.: DNA as substrate for packaging into bacteriophage Lambda in vitro. J. Mol. Biol. 98, 93–106 (1975)

    PubMed  CAS  Google Scholar 

  • Hopwood, D.A., Chater, K.F., Dowding, J.E., Vivian, A.: Advances in Streptomyces coelicolor genetics. Bacteriol. Rev. 37, 371–405 (1973)

    PubMed  CAS  Google Scholar 

  • Horiuchi, K., Zinder, N.D.: Cleavage of the bacteriophage fl DNA by the restriction enzyme of Escherichia coli B. Proc. Natl. Acad. Sci. U.S.A. 69, 3220–3224 (1972)

    PubMed  CAS  Google Scholar 

  • Hu, S., Ohtsubo, E., Davidson, N., Saedler , II.: Electron microscope heteroduplex studies of sequence relationships among bacterial plasmids: Identification and mapping of insertion sequences IS1 and IS2 in F and R plasmids. J. Bacteriol. 122, 764–775 (1975)

    PubMed  CAS  Google Scholar 

  • Inselburg, J.: The isolation, mapping and examination of effects of T insertions in Col plasmids. J. Bacteriol. 129, 482–491 (1977)

    PubMed  CAS  Google Scholar 

  • Jackson, D.A., Symons, R.H., Berg, P.: Biochemical method for inserting new genetic information into DNA of Simian virus 40: Circular SV40 DNA molecules containing Lambda phage genes and the galactose operon of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 69, 2904–2909 (1972)

    PubMed  CAS  Google Scholar 

  • Jacob, A.E., Grinter, N.J.: Plasmid RP4 as a vector replicon in genetic engineering. Nature 255, 504–506 (1975)

    PubMed  CAS  Google Scholar 

  • Jacob, A.E., Cresswell, J.M., Hedges, R.W., Coetzee, J.N., Beringer, J.E.: Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Molec. Gen. Genet. 147, 315–323 (1976)

    PubMed  CAS  Google Scholar 

  • Jacob, F., Wollman, E.L.: Les épisomes, éléments génétiques ajoutés. C. R. Inst. Past. 247, 154–156 (1958)

    CAS  Google Scholar 

  • Jacob, F., Brenner, S., Cuzin, F.: On the regulation of DNA replication in bacteria. Cold Spring Harbor Symp. Quant. Biol. 28, 329–348 (1963)

    CAS  Google Scholar 

  • Karrer, K.M., Gall, J.G.: The macro nuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome. J. molec. Biol. 104, 421–453 (1976)

    PubMed  CAS  Google Scholar 

  • Kaufmann, G., Kallenba, N.R.: Determination of recognition sites of T4 RNA ligase on 3’-OH and 5’-P termini of polynucleotide chains. Nature (Lond.) 254, 452–454 (1975)

    CAS  Google Scholar 

  • Kedes, L.H., Chang, A.C.Y., Houseman, D., Cohen, S.N.: Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature (Lond.) 255, 533–538 (1975)

    CAS  Google Scholar 

  • Kelly, T.J., Smith, H.O.: A restriction nuclease from Haemophilus influenzae. II. Base sequence of the recognition site. J. Molec. Biol. 51, 393–409 (1970)

    PubMed  CAS  Google Scholar 

  • Kingsbury, D.T., Helinski, D.R.: Temperature sensitive mutants for replication of plasmids in E. coli. I. Isolation and specificity of host and plasmid mutations. Genetics 74, 17–31 (1973)

    PubMed  CAS  Google Scholar 

  • Kirby, R., Wright, L.F., Hopwood, D.A.: Plasmid determined antibiotic synthesis and resistance in Streptomyces coelicolor. Nature (Lond.) 254, 265–270 (1975)

    CAS  Google Scholar 

  • Kleppe, K., Van De Sande, J.H., Khorana, H.G.: Polynucleotide ligase-catalysed joining of deoxyribo-oligonucleotide templates and of ribo-oligonucleotides on deoxyribopolynu cleotide templates. Proc. Natl. Acad. Sci. U.S.A. 67, 68–73 (1970)

    PubMed  CAS  Google Scholar 

  • Kolata, G.B.: Jumping genes: a common occurence in cells. Science 193, 392–394 (1976)

    PubMed  CAS  Google Scholar 

  • Kopecko, D.J., Cohen, S.N.: Site-specific re-independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc. Natl. Acad. Sci. U.S.A. 72, 1373–1377 (1975)

    PubMed  CAS  Google Scholar 

  • Kramer, R.A., Cameron, J.R., Davis, R.W.: Isolation of bacteriophage X containing yeast ribosomal RNA genes: screening by in situ RNA hybridisation to plaques. Cell 8, 227–232 (1976)

    PubMed  CAS  Google Scholar 

  • Kretschmer, P.J., Chang, A.C.Y., Cohen, S.N.: Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J. Bacteriol. 124, 225–231 (1975)

    PubMed  CAS  Google Scholar 

  • Landy, A., Ruedisueli, E., Robinson, L., Foeller, C., Ross, W.: Digestion of DNAs from bacteriophage T7, and 80 with site-specific nucleases from Haemophilus infulenzae strain Rc and strain Rd. Biochem. 13, 2134–2142 (1974)

    CAS  Google Scholar 

  • Last, J.A.: Purification and properties of RNA ligase from bacteriophage T4 infected E. coli. Fed. Proc. 34, 701 (1975)

    Google Scholar 

  • Lebowitz, P., Bloodgood, R.: Transcription of simian virus 40 DNA by Escherichia coli RNA polymerase: synthesis of a DNA-RNA hybrid and discrete RNAs under restrictive transcription conditions. J. Molec. Biol. 94, 183–201 (1975)

    PubMed  CAS  Google Scholar 

  • Lederberg, E.M., Cohen, S.N.: Transformation of Salmonella typhimurium by plasmid DNA. J. Bacteriol. 119, 1072–1074 (1974)

    PubMed  CAS  Google Scholar 

  • Lederberg, J.: Cell genetics and hereditary symbiosis. Physiol. Rev. 32, 403–430 (1952)

    PubMed  CAS  Google Scholar 

  • Lewin, B.: Sequence organisation of eukaryotic DNA. Defining the unit of gene expression. Cell 1, 107–111 (1974)

    CAS  Google Scholar 

  • Lieberman, R.P., Oishi, M.: Formation of the re-re DN’ase by in vitro complementation and evidence concerning its subunit nature. Nature [New Biol.] 243, 75–77 (1973)

    CAS  Google Scholar 

  • Lindahl, L., Nomura, M.: Analysis and organisation of Escherichia coli ribosome and RNA polymerase genes, using biochemical methods. In: Control of Ribosome Synthesis. Ninth Alfred Benzon Symp. Kjeldgaard, N.O., Maaloe, O. (eds.). Copenhagen: Munksgaard 1976, pp. 206–217

    Google Scholar 

  • Lobban, P.E., Kaiser, A.D.: Enzymatic end to end joining of DNA molecules. J. Molec. Biol. 78, 453–471 (1973)

    PubMed  CAS  Google Scholar 

  • Lovett, M.A., Helinski, D.R.: Relaxation complexes of plasmid DNA and protein. II. Characterisation of the proteins associated with the unrelaxed and relaxed complexes of plasmid Col. J. Biol. Chem. 250, 8790–8795 (1975)

    PubMed  CAS  Google Scholar 

  • Lovett, M.A., Helinski, D.R.: Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F, Km plasmid. J. Bacteriol. 127, 982–987 (1976)

    PubMed  CAS  Google Scholar 

  • Mandel, M., Higa, A.: Calcium-dependant bacteriophage DNA infection. J. Molec. Biol. 55, 159–162 (1970)

    Google Scholar 

  • Maniatis, T., Gek Kee, S., Efstratiadis, A., Kafatos, F.C.: Amplification and characterisation of a ß-globin gene synthesised in vitro. Cell 8, 163–182 (1976 a)

    PubMed  CAS  Google Scholar 

  • Maniatis, T., Efstratiadis, A., Gek Kee, S., Kafatos, F.C.: In vitro synthesis and molecular cloning of eukaryotic structural genes. In: ICN-UCLA Symp. Molec. Cell. Biol, Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976b

    Google Scholar 

  • Marians, K.J., Wu, R., Stawinski, J., Hozumi, T., Narang, A.: Cloned synthetic lac operator DNA is biologically active. Nature (Lond.) 265, 744–748 (1976)

    Google Scholar 

  • Matsubara, K, Takagi, Y., Mukai, T.: In vitro construction of different oligomeric forms of X DNA and studies on their transforming activities. J. Virol. 16, 479–485 (1975)

    PubMed  CAS  Google Scholar 

  • Matsubara, K: Genetic structure and regulation of a replicon of plasmid X. J. Molec. Biol. 102, 427–439 (1976)

    PubMed  CAS  Google Scholar 

  • Meagher, R.B., Tait, R.D., Betlach, M., Boyer, H.W.: Protein expression in E. coli minieells by recombinant plasmids. Cell 10, 521–536 (1977)

    PubMed  CAS  Google Scholar 

  • Mertz, J.E., Davis, R.W.: Cleavage of DNA by R1 restriction endonuclease generates cohesive ends. Proc. Natl. Acad. Sci. U.S.A. 69, 3370–3374 (1972)

    PubMed  CAS  Google Scholar 

  • Meynell, E., Meynell, G.G., Datta, N.: Phylogenetic relationships of the drug resistance factors and other transmissible bacterial plasmids. Bacteriol. Rev. 52, 55–83 (1968)

    Google Scholar 

  • Morrow, J.F., Cohen, S.N., Chang, A.C.Y., Boyer, H.W., Goodman, H.M., Helling, R.B.: Replication and transcription of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 71, 1743–1747 (1974)

    PubMed  CAS  Google Scholar 

  • Mukai, T., Matsubara, K, Takagi, Y.: In vitro construction of Col factor carrying genes for synthesis of guanine or thymine. Proc. Jpn. Acad. 51, 353–357 (1975)

    CAS  Google Scholar 

  • Mukai, T., Matsubara, K., Takagi, Y.: Cloning bacterial genes with plasmid X. Molec. Gen. Genet. 146, 269–274 (1976)

    PubMed  CAS  Google Scholar 

  • Mulder, C., Delius, H.: Specificity of the break produced by restricting endonuclease R1 in simian virus 40 DNA as revealed by partial denaturation mapping. Proc. Natl. Acad. Sci. U.S.A. 69, 3515–3219 (1972)

    Google Scholar 

  • Müller, W., Gautier, F.: Interactions of heteroaromatic compounds with nucleic acids; A.T.-specific non-intercalating DNA ligands. Eur. J. Biochem. 54, 385–394 (1975)

    PubMed  Google Scholar 

  • Murray, K, Murray, N.E., Brammar, W.J.: Restriction enzymes and the cloning of eukaryotic DNA. Proc. FEBS Meeting 10, 193–207 (1975)

    Google Scholar 

  • Nagel de Zwaig, R., Luria, S.E.: Genetics and physiology of colicin tolerant mutants of Escherichia coli. J. Bacteriol. 94, 1112–1123 (1967)

    Google Scholar 

  • Nagel de Zwaig, R., Luria, S.E.: New class of colicin tolerant mutants. J. Bacteriol. 99, 78–84 (1969)

    Google Scholar 

  • Nisioka, T., Mitani, M., Clowes, R.C.: Composite circular forms of R-factor deoxyribonucleic acid molecules. J. Bacteriol. 97, 376–385 (1969)

    PubMed  CAS  Google Scholar 

  • Nomura, M., Witten, C.: Interactions of colicins with bacterial cells. III. Colicin-tolerant mutations in Escherichia coli. J. Bacteriol. 94, 1093–1111 (1967)

    PubMed  CAS  Google Scholar 

  • Nordström, K, Ingram, C.L., Lundbäck, A.: Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome. J. Bacteriol. 97, 375–385 (1972)

    Google Scholar 

  • Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, R. III, Datta, N., Falkow, S.: Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol. Rev. 40, 168–189 (1976)

    PubMed  CAS  Google Scholar 

  • Ohtsubo, E., Soll, L., Deonier, R.C., Lee, H.J., Davidson, N.: Electron microscopic heteroduplex studies of sequence relations among plasmids of E. coli. VIII. The structure of phage f80d3 ilv su + l including the mapping of the ribosomal RNA. J. Molec. Biol. 89, 565–585 (1974)

    CAS  Google Scholar 

  • Oishi, M., Cosloy, S.D.: Specialised transformation in Escherichia coli Kl2. Nature (Lond.) 248, 112–116 (1974)

    CAS  Google Scholar 

  • Old, R.W., Murray, K., Roizes, G.: Recognition sequence of restriction endonuclease III from Haemophilus influenzae. J. Molec. Biol. 92, 331–339 (1975)

    PubMed  CAS  Google Scholar 

  • Padmanaban, G., Hendler, F., Patzer, J., Ryan, R., Rabinowitz, M.: Translation of RNA that contains polyadenylate from yeast mitochondria in an Escherichia coli ribosomal system. Proc. Natl. Acad. Sci. U.S.A. 72, 4293–4297 (1975)

    PubMed  CAS  Google Scholar 

  • Polisky, B., Greene, P., Garfin, D.E., Marthy, B.J., Goodman, H.M., Boyer, H.W.: Specificity of substrate recognition by the £cI restriction endonuclease. Proc. Natl. Acad. Sci. U.S.A. 72, 3310–3314 (1975)

    PubMed  CAS  Google Scholar 

  • Pritchard, R.H., Barth, P.T., Collins, J.: Control of DNA synthesis in bacteria. Symp. Soc. Gen. Microbiol. 14, 263–297 (1969)

    Google Scholar 

  • Pritchard, R.H., Chandler, M.G., Collins, J.: Independence of F replication and chromosome replication in Escherichia coli. Molec. Gen. Genet. 138, 143–155 (1975)

    PubMed  CAS  Google Scholar 

  • Pruss, G.J., Wang, J.C., Calendar, R.: In vitro packaging of covalently closed circular monomers of bacteriophage DNA. J. Molec. Biol. 98, 465–178 (1975)

    PubMed  CAS  Google Scholar 

  • Rabbits, T.H.: Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA. Nature (Lond.) 260, 221–225 (1976)

    Google Scholar 

  • Radioff, R., Bauer, W., Vinograd, J.: A dye bouyant-density method for the detection and isolation of closed circular duplex DNA: Closed circular DNA in He La cells. Proc. Natl. Acad. Sci. U.S.A. 57, 1514–1521 (1967)

    Google Scholar 

  • Roberts, R.J.: Restriction enzymes. CRC Crit. Rev. Biochem. 4, 123–164 (1976)

    PubMed  CAS  Google Scholar 

  • Roberts, R.J., Breitmeyer, J.B., Tabachnik, N.F., Myers, P.A.: A second specific nuclease from Haemophilus aegypticus. J. Molec. Biol. 91, 121–123 (1975)

    PubMed  CAS  Google Scholar 

  • Rodriguez, R.L., Bolivar, F., Goodman, H.M., Boy er, H.W., Betlach, M.: Construction and characterisation of cloning vehicles. In: ICN-UCLA Symp. Molec. Cell. Biol. Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976

    Google Scholar 

  • Rougeon, F., Kourilsky, P., Mach, B.: Insertion of a rabbit ß-globin gene sequence into an E. coli plasmid. Nucleic Acids Res. 2, 2365–2378 (1975)

    PubMed  CAS  Google Scholar 

  • Roy, P.H., Smith, H.O.: DNA methylases of Haemophilus influenzae Rd. II. Partial recognition site base sequences. J. Molec. Biol. 81, 445–459 (1973)

    PubMed  CAS  Google Scholar 

  • Roychoudhury, R., Jay, E., Wu, R.: Terminal labelling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 3, 101–116 (1976)

    PubMed  CAS  Google Scholar 

  • Schaffner, W., Gross, K., Telford, J., Birnstiel, M.: Molecular analysis of the histone gene cluster of Psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences. Cell 8, 471–478 (1976)

    PubMed  CAS  Google Scholar 

  • Schrempf H., Bujard, H., Hopwood, D.A., Goebel, W.: Isolation of covalently closed circular DNA from Streptomyces coelicolor A3(2). J. Bacteriol. 121, 416–421 (1975)

    PubMed  CAS  Google Scholar 

  • Sgaramella, V., Vand De Sande, J.H., Khorana, H.G.: Studies on polynucleotides. C.A. novel joining reaction catalysed by the T4-polynucleotide ligase. Proc. Natl. Acad. Sci. U.S.A. 67, 1468–1475 (1970)

    PubMed  CAS  Google Scholar 

  • Sharp, P.A., Gallimore, P.H., Flint, S.J.: Mapping of the adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harbor Symp. Quant. Biol. 41, 457–473 (1975)

    Google Scholar 

  • Shiu Lee, A., Constantini, F., Britten, R.J., Davidson, E.H.: Initial studies of sea urchin DNA sequence organisation by molecular cloning. In: ICN-UCLA Symp. Molec. Cell Biol Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976

    Google Scholar 

  • Smith, D.I., Blattner, F.R., Davies, J.: The isolation and partial characterisation of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res. 3, 343–353 (1976)

    PubMed  CAS  Google Scholar 

  • Smith, H.R., Humphreys, G.O., Willshaw, G.A., Anderson, E.S.: Characterisation of plasmids coding for the restriction endonuclease EcoR1. Molec. Gen. Genet. 143, 319–325 (1976)

    PubMed  CAS  Google Scholar 

  • Smith, J.D., Arber, W., Kuhnlein, U.: Host specificity of DNA produced by Escherichia coli. XIV. The role of nucleotide methylation in vivo in B-specific modification. J. Molec. Biol. 63, 1–8 (1972)

    PubMed  CAS  Google Scholar 

  • Snopek, T.J., Sugino, A., Agarwal, K.L., Cozzarelli, N.R.: Catalysis of DNA joining by bacteriophage T4 RNA ligase. Biochem. Biophys. Res. Commun. 68, 417–424 (1976)

    PubMed  CAS  Google Scholar 

  • So, M., Gill, R., Falkow, S.: The generation of a Col-A cloning vehicle which allows detection of inserted DNA. Molec. Gen. Genet. 142, 239–249 (1975)

    PubMed  CAS  Google Scholar 

  • Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Molec. Biol. 98, 503–517 (1975)

    PubMed  CAS  Google Scholar 

  • Spizizen, J.: Transformation of biochemically deficient strains of B. subtilis by deoxyribonu-cleate. Proc. Natl. Acad. Sci. U.S.A. 44, 1072–1078 (1958)

    PubMed  CAS  Google Scholar 

  • Staudenbauer, W.L.: Replication of Col in plasmolysed E. coli cells. Coupling of replication and RNA synthesis. Eur. J. Biochem. 58, 303–315 (1975)

    PubMed  CAS  Google Scholar 

  • Staudenbauer, W.L.: Replication of small plasmids. Curr. Top. Microbiol. Immunol. (1978) (In preparation)

    Google Scholar 

  • Steitz, J.A., Jakes, K.: How ribosomes select initiator regions in NA: base pair formation between the 3’ terminus of 16S NA and the NA during initiation of protein synthesis in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 72, 4734–4738 (1975)

    PubMed  CAS  Google Scholar 

  • Struhl, K., Cameron, J.R., Davis, R.W.: Functional genetic expression of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 73, 1471–1475 (1976)

    PubMed  CAS  Google Scholar 

  • Stuy, J.H.: Transformability of Haemophilus influenzae. J. Gen. Microbiol. 29, 537–549 (1962)

    PubMed  CAS  Google Scholar 

  • Syvanen, M.: Processing of bacteriophage Lambda DNA during its assembly into heads. J. Molec. Biol. 91, 165–174 (1975)

    PubMed  CAS  Google Scholar 

  • Tabak, H.F., Borst, P.: Erroneous transcription of mitochondrial DNA by RNA polymerase from Escherichia coli. Biochim. Biophys. Acta 217, 356–364 (1970)

    PubMed  CAS  Google Scholar 

  • Tait, R.C., Meagher, R.B., Goodman, H.W., Boyer, H.W.: The production of proteins by bacterial plasmids containing eukaryotic DNA fragments. In: ICN-UCLA Symp. Molec. Cell. Biol. Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976

    Google Scholar 

  • Tanaka, T., Weisblum, B.: Construction of a colicin El-R factor composite plasmid in vitro: Means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)

    PubMed  CAS  Google Scholar 

  • Thomas, M., White, R.L., Davis, R.W.: Hybridisation of RNA to double-stranded DNA: formation of R-loops. Proc. Natl. Acad. Sci. U.S.A. 73, 2294–2298 (1976)

    PubMed  CAS  Google Scholar 

  • Timmis, K., Cabello, F., Cohen, S.N.: Utilisation of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Proc. Natl. Acad. Sci. U.S.A. 71, 4556–4560 (1974)

    PubMed  CAS  Google Scholar 

  • Timmis, K., Cabello, F., Cohen, S.N.: Cloning, isolation, and characterisation of replicating regions of complex plasmid genomes. Proc. Natl. Acad. Sci. U.S.A. 72, 2242–2246 (1975)

    PubMed  CAS  Google Scholar 

  • Tyler, J., Sherratt, D.J.: Synthesis of E colicins in Escherichia coli. Molec. Gen. Genet. 140, 349–353 (1975)

    PubMed  CAS  Google Scholar 

  • Uhlin, B.E., Nordstrom, K.: Plasmid incompatibility and control of replication: copy mutants of the R factor R1 in E. coli K12. J. Bacteriol. 124, 641–649 (1975)

    PubMed  CAS  Google Scholar 

  • Venetianer, P., Leder, P.: Enzymatic synthesis of solid phasebound DNA sequences corresponding to specific mammalian genes. Proc. Natl. Acad. Sci. U.S.A. 71, 3892–3896 (1974)

    PubMed  CAS  Google Scholar 

  • Wackernagel, W.: Genetic transformation in E. coli: the inhibitory role of the recBC DNAse. Biochem. Biophys. Res. Commun. 51, 306–311 (1973)

    PubMed  CAS  Google Scholar 

  • Walker, G.C., Uhlenbec, O.C., Bedows, E., Gumport, R.I.: T4 induced RNA ligase joins single stranded oligoribonucleotides. Proc. Natl. Acad. Sci. U.S.A. 72, 122–126 (1975)

    PubMed  CAS  Google Scholar 

  • Watanabe, T., Fukasawa, T., Takano, T.: Conversion of male bacteria of Escherichia coli K12 to resistance to f phages by infection with the episome “resistance transfer factor”. Virology 17, 217–219 (1962)

    PubMed  CAS  Google Scholar 

  • Watanabe, T.: Infectious drug resistance in bacteria. Curr. Top. Microbiol. Immunol. 56, 43–98 (1971)

    PubMed  CAS  Google Scholar 

  • Wensink, P.C., Finnegan, D.J., Donelson, J.E., Hogness, D.S.: A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell 3, 315–325 (1974)

    PubMed  CAS  Google Scholar 

  • Wohlheiter, J.A., Falkow, S., Citarella, R.V., Baron, L.S.: Characterisation of DNA from a proteus strain harboring an episome. J. Molec. Biol. 9, 576–588 (1964)

    Google Scholar 

  • Wood, K.O., Lee, J.C.: Integration of synthetic globin genes into an E. coli plasmid. Nucleic Acids Res. 3, 1961–1971 (1976)

    PubMed  CAS  Google Scholar 

  • Yang, H-L., Zubay, G., Levy, S.B.: Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated. Proc. Natl. Acad. Sci. U.S.A. 73, 1509–1512 (1976)

    PubMed  CAS  Google Scholar 

  • Zaritsky, A., Pritchard, R.H.: Replication time of the chromosome in thymineless mutants of Escherichia coli. J. Molec. Biol. 60, 65–74 (1971)

    PubMed  CAS  Google Scholar 

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  1. Gesellschaft für Biotechnologische Forschung mbH, D-3300, Braunschweig-Stöckheim, Federal Republic of Germany

    John Collins

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    W. Arber

  2. Philadelphia, USA

    W. Henle

  3. Martinsried, Germany

    P. H. Hofschneider

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    L. Syruček

  13. Los Angeles, USA

    P. K. Vogt

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Collins, J. (1977). Gene Cloning with Small Plasmids. In: Arber, W., et al. Current Topics in Microbiology and Immunology. Current Topics in Microbiology and Immunology, vol 78. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66800-5_5

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