Abstract
The objectives of this study are to assess the influences of land use and of heavy metal loads on the soil microbial community. For this purpose we collected soils from 27 sites throughout Switzerland and analyzed saturated, mono- and polyunsaturated PLFAs (phospholipid fatty acids), ATP (total adenylates) and the total content of the heavy metal cadmium. We found a positive correlation between the sum of PLFA and ATP. Further, we found that land use and cadmium load are reflected by PLFA contents. Based on the organismic origin we formed guilds of individual PLFAs. These guilds relate to taxonomic units and trophic function at the same time. Therefore, it is possible to infer the structure (structural diversity) and trophic diversity (functional diversity) of the soil microbial communities. We found a negative correlation between functional diversity and cadmium load. Functional diversity differentiated also land use types. PLFA analysis may reveal structural and functional in situ properties of whole soil microbial communities. Substrate utilization tests, which are restricted to cultivable organisms, predominantly bacteria and fungi, may supplement PLFA analysis with data about specific activities. Finally, our results indicate that PLFA analysis can be used to estimate critical pollutant loads.
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References
Albers B, Zelles L, Bai Q, Lörinci G, Hartmann A, Beese F (1994) Fettsauremuster von Phospholipiden und Lipopolysacchariden fur die Struktur von Mikroorganismengesellschaften in Boden. In: Alef K, Fiedler H, Hutzinger O (eds) ECOINFORMA 5, Umweltbundesamt, Berlin, pp. 297–312.
Atteia O, Thélin HR, Pfeifer, Dubois JP, Hunziker JC (1995) A search for the origin of cadmium in the soil of the Swiss Jura. Geoderma 68: 149–172.
Baath E, Frostegard A, Fritze H (1992) Soil bacterial biomass, activity, phospholipid fatty acid pattern, and pH tolerance in an area polluted with alkaline dust deposition. Appl Environ Microbiol 58: 4026–4031.
Baur B, Joshi J, Schmid B, Hanggi A, Borcard D, Stray I, Pedroli A, Thommen H, Luka H, Rusterholz H, Oggier P, Ledergerber S, Erhardt A (1996) Variation in species richness of plants and diverse groups of invertebrates in three calcareous grasslands of the Swiss Jura mountains. Revue suisse de Zoologie 103: 801–833.
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911–917.
Bono R (1993) Schwermetalle in den Boden des Kantons Basel-Landschaft Amt fur Umweltschutz und Energie, Liestal.
Burkhardt C, Insam H, Hutchinson TC, Reber HH (1993) Impact of heavy metals on the degradative capabilities of soil bacterial communities. Biol Fertil Soils 16: 154–156.
Dean-Ross D, Mills AL (1989) Bacterial community structure and function along a heavy metal gradient. Appl Environ Microbiol 55: 2002–2009.
Domsch KH (1985) Funktionen und Belastbarkeit des Bodens aus der Sicht der Bodenmikrobiologie. Rat der Sachverstandigen fur Umweltfragen. Verlag W. Kohlhammer, Stuttgart und Mainz.
FAC (1989) Methoden fur Bodenuntersuchungen. Schriftenreihe der FAC Liebefeld Nr. 5, Liebefeld-Bern
Hassink J, Neutel AM, de Ruiter PC(1994) C and N mineralization in sandy and loamy grassland soils: the role of microbes and micro fauna, Soil Biol Biochem 26; 1565–1571
Hill MO (1973) Diversity and evenness: a unifying notation and its consequences. Ecology 54: 427–432.
Hunt HW, Coleman DC, Ingahm ER, Eliott ET, Moore IC, Rose SL, Reid CPP, Morley CR (1987) The detrital food web in a shortgrass prairie. Biol Fertil Soils 3: 57–68.
Huston MA (1994) Biological Diversity. Cambridge University Press.
Korner J, Laczko E (1992) A new method for assessing soil microorganisms diversity and evidence of vitamin deficiency in low diversity communities. Biol Fertil Soils 13: 58–60.
Maire N (1982) Methode de mesure de l’adenosine triphosphat (ATP) dans les sols. Bulletin de la Societe Suisse de Pedologie 6: 88–94.
Maire N (1984) Extraction de l’adenosin triphosphat dans les sols: une nouvelle methode de calcul des pertes en ATP. Soil Biol Biochem 16: 361–366.
Olsson PA, Baath E, Jakobsen I, Söderström B (1995) The use of phospholipid and neutral fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil. Mycol Res 99: 623–629
Pennanen T, Frostegard A, Fitze H, Baath E (1996) Phospholipid fatty acid composition and heavy metal tolerance of soil microbial communities along two heavy metal-polluted gradients in coniferous forests. Appl Environ Microbiol 62: 420–428.
Ratledge C, Wilkinson SG (1988) Microbial Lipids. Academic Press, London.
Rudaz A, Brüning E (1990). Importance et contribution des microorgnismes pour l’élaboration de critères de fertilité des sols. Recherche agronomique en Suisse 29: 16–22.
Suzuki K, Goodfellow M, O’Donnel AG (1993) Cell envelopes and classification. In: Goodfellow M, O’Donnel AG (eds) Handbook of New Bacterial Systematics, Academic Press, London, pp. 195–250.
Vestal JR, White DC (1989) Lipid analysis in microbial ecology. Bioscience 39: 535–541.
Vogel H, Desaules A, Hani H. (1989) Schwermetallgehalte in den Boden der Schweiz. Bericht 40 des Nationalen Forschungsprogrammes Boden, Liebefeld-Bern.
White DC (1995) Chemical ecology: possible linkage between macro- and microbial ecology. Oikos 74: 177–184.
Zelles L, Bai QY, Beck T, Beese F (1992) Signature fatty acids in phospholipids and lipopolysaccharides as indicators of microbial biomass and community structure in agricultural soils. Soil Biol Biochem 24: 317–323.
Zelles L, Bai QY (1993) Fractionation of fatty acids derived from soil lipids by solid phase extraction and their quantitative analysis by GC-MS. Soil Biol Biochem 25: 495–507.
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© 1997 Springer-Verlag Berlin Heidelberg
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Laczko, E., Rudaz, A., Aragno, M. (1997). Diversity of Anthropogenically Influenced or Disturbed Soil Microbial Communities. In: Insam, H., Rangger, A. (eds) Microbial Communities. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60694-6_6
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DOI: https://doi.org/10.1007/978-3-642-60694-6_6
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