Abstract
Electric field sensitive dyes allow electrical events in biological membranes to be detected optically by converting changes in electric field strength into a fluorescence or UV absorbance response. Their response mechanisms to a change in electric field can involve movement of the dye as a whole (either across or within the membrane) or the movement of the dye’s electrons, with the mechanism followed by a particular dye depending on its molecular structure. The response times can vary from nanoseconds (for electron movement) to seconds (for dye movement across the entire membrane). Applications of the dyes include the quantification of plasma membrane potential, the surface potential, and the intramembrane dipole potential, as well as following the kinetic activity of electrogenic ion pumps, such as the Na+,K+-ATPase.
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Clarke, R.J. (2010). Electric Field Sensitive Dyes. In: Demchenko, A. (eds) Advanced Fluorescence Reporters in Chemistry and Biology I. Springer Series on Fluorescence, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-04702-2_10
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DOI: https://doi.org/10.1007/978-3-642-04702-2_10
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