Abstract
Presently, most of the alginate produced commercially is still obtained from algae, although it is subjected to variations in quality and quantity due to changes of climate and sources. Alginate can also be biotechnologically produced by species of two families of heterotrophic bacteria, Pseudomonas and Azotobacter. Efforts have been made in the past to produce alginate-like polymers from these bacteria. The association of virulence with alginate production in most Pseudomonas spp. has made Azotobacter vinelandii the most promising candidate for the industrial production of alginate. Nevertheless, for specific and well-defined applications, especially in biomedical and pharmaceutical fields, the production of Pseudomonas alginate has attracted increasing attention. Microbial alginate production has been widely investigated in batch, fed-batch, and continuous cultures. This chapter summarizes current knowledge of physiology and process aspects in view of potential industrial production of microbial alginate.
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References
Alkawash MA, Soothill JS, Schiller NL (2006) Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms. APMIS 114(2):131–138
Amici E, Tetradis-Meris G, Pulido de Torrres C, Jousse F (2008) Alginate gelation in microfluidic channels. Food Hydrocolloids 22:97–104
Annison G, Couperwhite I (1986a) Influence of calcium on alginate production and composition in continuous cultures of Azotobacter vinelandii. Appl Microbiol Biotechnol 25:55–61
Annison G, Couperwhite I (1986b) Effect of limiting substrate concentration, growth rate and aeration on alginate composition and production by Azotobacter vinelandii in continuous culture. Food Hydrocolloids 1(2):101–111
Anwar H, Strap JL, Costerton JW (1992) Establishment of aging biofilms: possible mechanisms of bacterial resistance to antimicrobial therapy. Antimicrob Agent Chemother 36:1347–1351
Bayer AS, Eftekhar F, Tu J, Nast CC, Speert DP (1990) Oxygen-dependent up- regulation of mucoid exopolysaccharide (alginate) production in Pseudomonas aeruginosa. Infect Immun 58(5):1344–1349
Boiardi JL (1994) Metabolic cost of nitrogen incorporation by N2-fixing Azotobacter vinelandii is affected by the culture pH. Biotechnol Lett 16(11):1195–1198
Boyd A, Chakrabarty AM (1995) Pseudomonas aeruginosa biofilms: Role of the alginate exopolysaccharide. J Ind Microbiol 15:162–168
Brivonese A, Sutherland WI (1989) Polymer production by a mucoid strain of Azotobacter vinelandii in batch culture. Appl Microbiol Biotechnol 30:97–102
Chang WS, van de Mortel M, Nielsen L, Nino de Guzman G, Li X, Halverson LJ (2007) Alginate production by Pseudomonas putida creates a hydrated microenvironment and contributes to biofilm architecture and stress tolerance under water-limiting conditions. J Bacteriol 89(22):8290–8299
Chen WP, Chen JY, Chang SC, Su CL (1985) Bacterial alginate produced by a mutant of Azotobacter vinelandii. Appl Environ Microbiol 49(3):543–546
Clementi F, Fantozzii P, Mancini F, Moresi M (1995) Optimal conditions for alginate production by Azotobacter vinelandii. Enz Microbiol Technol 17(11):983–988
Clementi F, Crudele MA, Parente E, Mancini M, Moresi M (1999) Production and characterisation of alginate from Azotobacter vinelandii. J Sci Food Agric 79:602–610
Couperwhite I, McCallum MF (1974) The influence of EDTA on the composition of alginate synthesized by Azotobacter vinelandii. Arch Microbiol 97:73–80
Croft L, Beatson SA, Whitchurch CB, Huang B, Blakeley RL, Mattick JS (2000) An interactive web-based Pseudomonas aeruginosa genome database: Discovery of new genes, pathways and structures. Microbiology 146:2351–2364
Deavin L, Jarman TR, Lawson CJ, Richelato RC, Slocombe S (1977) The production of alginic acid by Azotobacter vinelandii in batch and continuous culture. In: Sanford PA, Laskin A (eds) Extracellular microbial polysaccharides. American Chemical Society, Washington, pp 14–26
Deckwer W-D (1992) Bubble column reactors. Wiley, Chichester
Díaz-Barrera A, Peña C, Galindo E (2007) The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii. Appl Microbiol Biotechnol 76(4):903–910
Doggett RG, Harrison GM, Stillwell RM, Wallis ES (1966) An atypical Pseudomonas aeruginosa associated with cystic fibrosis of the pancreas. J Pediatr 68:215–221
Dussap C-G, Gros J-B (1985) Power input, KLa values in pneumatically agitated fermentors with extracellular microbial polysaccharides. In: Biotechnologie 85, Europe, pp 691–692
Duyvis M, Wassink H, Haaker H (1998) Nitrogenase of Azotobacter vinelandii: kinetic analysis of the Fe protein redox cycle. Biochemistry 37:17345–17354
Ferrala NF, Westervelt P, Mabbott GA, Fekete FA (1986) Relation between extracellular polysaccharide production and medium iron concentration in nitrogen fixing Azotobacter chrococcum B-8. Abstr Annu Meet Am Soc Microbiol 86:Meeting K-143
Fett WF, Wells JM, Cescutti P, Wijey C (1995) Identification of exopolysaccharides produced by fluorescent pseudomonads associated with commercial mushroom (Agaricus bisporus) production. Appl Environ Microbiol 61:513–517
Fialh AM, Zielinski NA, Fett WF, Chakrabarty AM, Berry A (1990) Distribution of alginate gene sequences in the Pseudomonas rRNA homology group I-Azomonas-Azotobacter lineage of superfamily B procaryotes. Appl Environ Microbiol 56:436–443
Fyfe JAM, Govan JRW (1983) Synthesis, regulation and biological function of bacterial alginate. Prog Ind Microbiol 18:45–83
Galindo E, Peña C, Núñez C, Segura D, Espín G (2007) Molecular and bioengineering strategies to improve alginate and polydydroxyalkanoate production by Azotobacter vinelandii. Microb Cell Fact 6:7
Garcia-Ochoa F, Gomez E (2005) Prediction of gas-liquid mass transfer coefficient in sparged stirred tank bioreactors. Biotechnol Bioeng 92(6):761–772
Goh CH, Heng PW, Huang EP, Li BK, Chan LW (2008) Interactions of antimicrobial compounds with cross-linking agents of alginate dressings. J Antimicrob Chemother 62(1):105–108
Gorin JPA, Spencer TJF (1966) Exocellular alginic acid from Azotobacter vinelandii. Can J Chem 44:993–998
Gottschalk G (1988) Fixation of molecular nitrogen. In: Gottschalk G (ed) Bacterial metabolism, vol 10. Springer, Berlin, pp 318–326
Govan JR, Deretic V (1996) Microbial pathogensis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev 60(3):539–574
Govan JR, Fyfe MJA (1978) Mucoid Pseudomonas aerugionosa and cystic fibrosis: resistance of the mucoid form to carbencillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro. J Antimicrob Chemother 4:233–240
Haddock BA, Jones CW (1977) Bacterial respiration. Bacteriol Rev 41(1):47–99
Hammad AMM (1998) Evaluation of alginate encapsulated Azotobacter chroococcum as a phage-resistant and an effective inoculum. J Basic Microbiol 1:9–16
Holdal HK, Svanem IG, Gimmestad M, Valla S (2000) Mannuronan C-5 epimerases and cellular differentiation of Azotobacter vinelandii. Environ Microbiol 2(1):27–38
Horan NJ, Jarman TR, Dawes EA (1981) Effects of carbon source and inorganic phosphate concentration on the production of alginic acid by a mutant of Azotobacter vinelandii and on the enzyme involved in its biosynthesis. J Gen Microbiol 127:185–191
Horan NJ, Jarman TR, Dawes EA (1983) Studies on some enzymes of alginic acid biosynthesis in Azotobacter vinelandii grown in continuous culture. J Gen Microbiol 129:2985–2990
Jackson KD, Starkey M, Kremer S, Parsek MR, Wozniak DJ (2004) Identification of psl, a locus encoding a potential exopolysaccharide that is essential for Pseudomonas aeruginosa PAO1 biofilm formation. J Bacteriol 186:4466–4475
Jarman TR, Deavin I, Slocombe S, Righelato RC (1978) Investigation of the effect of environmental conditions on the rate of exopolysaccharides synthesis in Azotobacter vinelandii. J Gen Microbiol 107:59–64
Kachlany SC, Levery SB, Kim JS, Reuhs BL, Lion LW, Ghiorse WC (2001) Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7. Environ Microbiol 3:774–784
Kidambi PS, Sundin GW, Palmer AD, Chakrabarty MA, Bender LC (1995) Copper as a signal for alginate synthesis in Pseudomonas syringae pv. syringae. Appl Environ Microbiol 61(6):2172–2179
Kim EJ, Sabra W, Zeng AP (2003) Iron deficiency leads to inhibition of oxygen transfer and enhanced formation of virulence factors in cultures of Pseudomonas aeruginosa PAO1. Microbiology 149(9):2627–2634
Klimek J, Ollis DF (1980) Extracellular microbial polysaccharides: kinetics of Pseudomonas sp, Azotobacter vinelandii and Aureobasidium pullulans batch fermentations. Biotechnol Bioeng 22:2321–2342
Krieg DP, Bass JA, Mattingly SJ (1986) Aeration selects for mucoid phenotype of Pseudomonas aeruginosa. J Clin Microbiol 24(6):986–990
Kuhla J, Oelze J (1988) Dependence of nitrogenase switch-off upon oxygen stress on the nitrogenase activity in Azotobacter vinelandii. J Bacteriol 170(11):5325–5329
Laue H, Schenk A, Li H, Lambertsen L, Neu TR, Molin S, Ullrich MS (2006) Contribution of alginate and levan production to biofilm formation by Pseudomonas syringae. Microbiology 152(10):2909–2918
Lebrun L, Junter G-A, Jouenne T, Mignot L (1994) Exopolysaccharide production by free and immobilized microbial cultures. Enzyme Microb Technol 16:1048–1054
Leitäo JH, Sa-Correia I (1997) Oxygen dependant upregulation of transcription of alginate genes algA, algC and algD in Pseudomonas aeruginosa. Res Microbiol 148:37–43
Lichtl RJ, Bazin MO, Hall D (1997) The biotechnology of hydrogen production by Nostoc flaelliforme grown under chemostat conditions. Appl Microbiol Biotechnol 47:701–707
Linker A, Jones RS (1966) A polysaccharide resembling alginic acid from a Pseudomonas microorganism. Nature 204:187–188
Linkerhägner K, Oelze J (1995) Cellular ATP level and nitrogenase switchoff upon oxygen stress in chemostat cultures of Azotobacter vinelandii. J Bacteriol 177(18):5289–5293
Linkerhägner K, Oelze J (1997) Nitrogenase activity and regeneration of the cellular ATP pool in Azotobacter vinelandii adapted to different oxygen concentrations. J Bacteriol 179(4):1362–1367
Liu JK, Lee FT, Lin CS, Yao XT, Davenport JW, Wong TY (1995) Alternative function of the electron transport system in Azotobacter vinelandii: Removal of excess reductant by the cytochrome d pathway. Appl Environ Microbiol 61(11):3998–4003
Luedeking R, Piret EL (1959) A kinetic study of lactic acid fermentation. Batch process at controlled pH. J Biochem Microbial Technol Eng 1:393–431
Ma S, Selvaraj U, Ohman DE, Quarless R, Hassett DJ, Wazniak DJ (1998) Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in Mucoid Pseudomonas aeruginosa. J Bacteriol 180(4):956–968
Mancini M., Moresi M, Sappino F (1996). Rheological behaviour of aqueous dispersions of algal sodium alginates. J Food Eng 28:238–295
Margaritis A, Pace GW (1985) Microbial polysaccharides. In: Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology, vol 3. Pergmann, Oxford, pp 1005–1044
Martin DR (1973) Mucoid variation in Pseudomonas aeruginosa induced by the action of phage. J Med Microbiol 6:111–118
Mathee K, Ciofu O, Sternberg C, Lindum PW, Campbell JIA, Jensen P, Johnsen A, Givskov M, Ohman DE, Molin S, Hoiby N, Kharazmi A (1999) Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: A mechanism for virulence activation in the cystic fibrosis lung. Microbiology 145:1349–1357
McNeil B, Harvey LM (1993) Viscous fermentation products. Crit Rev Biotechnol 13(4):275–304
Moshiri F, Crouse BR, Johnson MK, Maier RJ (1995) The nitrogenase protective FeSII protein of Azotobacter vinelandii: Over expression, characterisation and crystallisation. Biochemistry 34:12973–12982
Noguez R, Segura D, Moreno S, Hernandez A, Juarez K, Espín G (2008) Enzyme I, NPr and IIA(Ntr) are involved in regulation of the poly-beta-hydroxybutyrate biosynthetic genes in Azotobacter vinelandii. J Mol Microbiol Biotechnol 15:244–254
Nunez C, Moreno S, Soberon-Chavez G, Espin G (1999) The Azotobacter vinelandii response regulator AlgR is essential for cyst formation. J Bacteriol 181(1):141–148
Obika H, Sakakibara J, Kobayashi Y (1993) Direct control of the constituent ratio in a wide range in alginate produced by Azotobacter vinelandii. Biosci Biotechnol Biochem 57(2):332–333
Okabe E, Nakayima M, Murooka H, Nisizawa K (1981) Investigation of carbon and phosphorous sources in cultural media of a selected strain of alginate producing Azotobacter vinelandii. J Ferment Technol 59(1):1–7
Osman SF, Fett WF, Fishman ML (1986) Exopolysaccharides of the phytopathogen Pseudomonas syringae pv. glycinea. J Bacteriol 166:66–71
Parente E, Crudele MA, Aquino M, Clementi F (1998) Alginate production by Azotobacter vinelandii DSM576 in batch fermentation. J Ind Microbiol Biotechnol 20:171–176
Pena C, Campos N, Galindo E (1997) Evolution of alginate molecular weight distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks. Appl Microbiol Biotechnol 48:510–515
Pena C, Trujillo-Roldan MA, Galindo E (2000) Influence of dissolved oxygen tension and agitation speed on alginate production and its molecular weight in cultures of Azotobacter vinelandii. Enzyme Microb Technol 27:390–398
Peña C, Galindo E, Díaz M (2002) Effectiveness factor in biological external convection: study in high viscosity systems. J Biotechnol 95(1):1–12
Pena C, Milla M, Galindo E (2008). Production of alginate by Azotobacter vinelandii in a stirred fermentor simulating the evolution of power input observed in shake flasks. Process Biochem 43:775–778
Post E, Golecki RJ, Oelze J (1982) Morphological and ultrastructural variations in Azotobacter vinelandii growing in oxygen-controlled continuous culture. Arch Microbiol 133:75–82
Post E, Kleiner D, Oelze J (1983) Whole cell respiration and nitrogenase activities in Azotobacter vinelandii growing in oxygen controlled continuous culture. Arch Microbiol 134:68–72
Postgate JR (1971) Nitrogen fixation by free-living microbes. In: Postgate JR (ed) The chemistry and biochemistry of nitrogen fixation, vol 5. Plenum, London, pp 161–187
Postgate JR (1974) Evolution within nitrogen fixing systems. Symp Soc Gen Microbiol 24:263–242
Priester JH, Olson SG, Webb SM, Neu MP, Hersman LE, Holden PA (2006) Enhanced exopolymer production and chromium stabilization in Pseudomonas putida unsaturated biofilms. Appl Environ Microbiol 72:1988–1996
Pritt B, O’Brien L, Winn W (2007) Mucoid Pseudomonas in cystic fibrosis. Am J Clin Pathol 128(1):32–34
Qiu D, Eisinger VM, Head NE, Pier GB, Yu HD (2008) ClpXP proteases positively regulate alginate overexpression and mucoid conversion in Pseudomonas aeruginosa. Microbiology 154(7):2119–2130
Remminghorst U, Rehm BH (2006) In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa. Appl Environ Microbiol 72(1):298–305
Reyes C, Peña C, Galindo E (2003) Reproducing shake flasks performance in stirred fermentors: production of alginates by Azotobacter vinelandii. J Biotechnol 105:189–198
Sabra WA (1998) Microaerophilic production of alginate by Azotobacter vinelandii. Dissertation, Technische Universität Carolo-Wilhelmina zu Braunschweig, Braunschweig, http://www.biblio.tu-bs.de/ediss/data/19990415a/19990415a.html
Sabra WA, Hassan M (2008) Exopolysaccharide yield as a kinetic parameter for the statistical optimization of EPS production by Klebsiella pneumoniae biotechnology. ASCI 7(1):27–34
Sabra WA, Zeng A-P, Sabry S, Omar S, Deckwer W-D (1999) Effect of phosphate and oxygen concentrations on alginate production and stoichiometry of metabolism of Azotobacter vinelandii under microaerobic conditions. Appl Microbiol Biotechnol 52:773–780
Sabra WA, Zeng A-P, Lünsdorf H, Deckwer W-D (2000) Function and variation of alginate production in Azotobacter vinelandii under nitrogen fixation conditions. Appl Environ Microbiol 66(9):4037–4044
Sabra WA, Zeng A-P, Deckwer W-D (2001) Bacterial alginate: Physiology, product quality and process aspects. Appl Microbiol Biotechnol 56:315–325
Sabra WA, Kim E-J, Zeng A-P (2002) Physiological responses of Pseudomonas aeruginosa PA01 to oxidative stress in controlled microaerobic and aerobic cultures. Microbiology 148:3195–3202
Sabra WA, Lünsdorf H, Zeng A-P (2003) Alterations in the formation of lipopolysaccharide and membrane vesicles on the surface of Pseudomonas aeruginosa PAO1 under oxygen stress conditions. Microbiology 149:2789–2795
Sato SS, Mukataka H, Kataoka, Takahashi J (1984) Effects of pressure and dissolved oxygen concentration on growth of Pseudomonas aeruginosa. J Ferment Technol 62(1):71–75
Saude N, Junter G-A (2002) Production and molecular weight characteristics of alginate from free and immobilized-cell cultures of Azotobacter vinelandii. Process Biochem 37:895–900
Saude N, Chèze-Lange H, Beunard D, Dhulster P, Guillochon D, Cazé A-M, Morcellet M, Junter GA (2002) Alginate production by Azotobacter vinelandii in a membrane bioreactor. Process Biochem 38:273–278
Savalgi V, Savalgi V (1992) Alginate production by Azotobacter vinelandii in batch culture. J Gen Appl Microbiol 38:641–645
Schenk A, Weingart H, Ullrich MS (2008) The alternative sigma factor AlgT, but not alginate synthesis, promotes in planta multiplication of Pseudomonas syringae pv. glycinea. Microbiology 154(2):413–421
Schurr MJ, Martin DW, Mudd MH, Deretic V (1994) Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy. J Bacteriol 176(11):3375–3382
Seanez G, Pena C, Galindo E (2001) High CO2 affects alginate production and prevents polymer degradation in cultures of Azotobacter vinelandii. Enzyme Microbial Technol 29:535–540
Skjak-Braek G (1992) Alginate: biosynthesis and some structure function relationships relevant to biomedical and biotechnological applications. Biochem Soc Trans 20(1):27–33
Stehling EG, Silveira WD, Leite Dda S (2008) Study of biological characteristics of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis and from patients with extra-pulmonary infections. Braz J Infect Dis 12(1):86–88
Steigedal M, Sletta H, Moreno S, Maerk M, Christensen BE, Bjerkan T, Ellingsen TE, Espìn G, Ertesvåg H, Valla S (2008) The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation. Environ Microbiol 10(7):1760–1770
Suh IS, Schumpe A, Deckwer WD (1992) Xanthan production in bubble column and air- lift reactors. Biotechnol Bioeng 39(1):85–94
Trujillo-Roldan M, Pena C, Ramirez OT, Galindo E (2001) Effect of oscillating dissolved oxygen tension on the production of alginate by Azotobacter vinelandii. Biotechnol Prog 17:1042–1048
Trujillo-Roldán MA, Peña C, Galindo E (2003) Components in the inoculum determine the kinetics of Azotobacter vinelandii cultures and the molecular weight of its alginate. Biotechnol Lett 25(15):1251
Wagner VE, Iglewski BH (2008) P. aeruginosa Biofilms in CF infection. Clin Rev Allergy Immunol 35:124–134
Wood LF, Leech AJ, Ohman DE (2006) Cell wall-inhibitory antibiotics activate the alginate biosynthesis operon in Pseudomonas aeruginosa: roles of sigma (AlgT) and the AlgW and Prc proteases. Mol Microbiol 62(2):412–426
Xu KD, Stewart PS, Xia F, Huang C-T, McFeters GA (1998) Spatial physiological heteroginicity in Pseudomonas aeruginosa biofilm is determined by oxygen availability. Appl Environ Microbiol 64(10):4035–4039
Young JM, Park DC (2007) Probable synonymy of the nitrogen-fixing genus Azotobacter and the genus Pseudomonas. Int J Syst Evol Microbiol 57(12):2894–2901
Young M (1983) Microbial polysaccharide. In comprehensive biotechnology, vol 3. Pergamon, Toronto, pp 1005–1044
Yuan LL, Li YQ, Wang Y, Zhang XH, Xu YQ (2008) Optimization of critical medium components using response surface methodology for phenazine-1-carboxylic acid production by Pseudomonas sp. M-18Q. J Biosci Bioeng 105(3):232–237
Zhang ZY, Zhong JJ (2004) Scale-up of centrifugal impeller bioreactor for hyperproduction of ginseng saponin and polysaccharide by high-density cultivation of panax notoginseng cells. Biotechnol Prog 20(4):1076–1081
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Sabra, W., Zeng, A. (2009). Microbial Production of Alginates: Physiology and Process Aspects. In: Rehm, B. (eds) Alginates: Biology and Applications. Microbiology Monographs, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-92679-5_7
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