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Advances in Applied Bioremediation pp 315–338Cite as

The Environment and the Tools in Rhizo- and Bioremediation of Contaminated Soil

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Part of the book series: Soil Biology ((SOILBIOL,volume 17))

Abstract

Several methods are available for determining microbial diversity in the soil environment, including different plating methods, light and fluorescence microscopy, and DNA and RNA analysis. Since culturable proportions of bacterial communities from the environment are negligible compared with the number of species that are present in the soil environment, culture techniques for environmental bacterial community diversity analysis are becoming less popular. Molecular techniques have been developed to identify and determine species diversity of microorganisms without isolation. Molecular techniques also provide a rapid means of determining the relative abundance of common species present in a given sample, which do not need to be culturable. Characterization of genes involved in the degradation of organic pollutants has also led to the application of molecular techniques in the microbial ecology of polluted areas.

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Authors and Affiliations

  1. Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South Africa

    A. K. J. Surridge, F. C. Wehner & T. E. Cloete

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  1. A. K. J. Surridge
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  2. F. C. Wehner
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  3. T. E. Cloete
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Correspondence to A. K. J. Surridge .

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Editors and Affiliations

  1. Dept. Biology, University of Waterloo, University Avenue W. 200, Waterloo, N2L 3G1, Canada

    A. Singh

  2. Fac. Inter-Disciplinary &, University of Delhi, Benito Juarez Road, New Delhi, 110021, India

    Ramesh C. Kuhad

  3. Dept. Biology, University of Waterloo, University Avenue W. 200, Waterloo, N2L 3G1, Canada

    Owen P. Ward

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Surridge, A.K.J., Wehner, F.C., Cloete, T.E. (2009). The Environment and the Tools in Rhizo- and Bioremediation of Contaminated Soil. In: Singh, A., Kuhad, R., Ward, O. (eds) Advances in Applied Bioremediation. Soil Biology, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-89621-0_17

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