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Microbial Biosensors for Metal(loid)s

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Microbial Ecotoxicology

Abstract

In this chapter we carry out an updated review on metal(loid)s biosensors using microorganisms as bioreceptor element of a classic biosensor or as a whole-cell biosensor. We analyze the potential advantages and possible disadvantages to use prokaryotic or eukaryotic microorganisms in metal(loid) biosensors. Likewise, the presence or absence of a cell wall in the microbial system can determine the degree of permeability of the target molecule to be detected. Sensitivity versus specificity of the biosensor is also discussed. We call attention on the necessity to carry out more bioassays using real environmental samples, and not only laboratory prepared once. A greater interest on designing biosensors using protozoa is also reclaimed, because these eukaryotic microorganism are much more sensitive to metal(loid)s than other microorganisms, and they share a higher degree of functional conservation with human genes than do other eukaryotic microbial models. Finally, a collection and analysis of the main metal(loid) microbial biosensors and genetic constructs potentially useful to design metal biosensors is reported.

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Abbreviations

CB:

Classical or conventional biosensor/s

GFP:

Green fluorescent protein

MT:

Metallothionein

WCB:

Whole-cell biosensor/s

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Authors and Affiliations

  1. Dpto. Microbiología-III, Facultad de Biología, Universidad Complutense (UCM), C/ José Antonio Novais, Nº 12, 28040, Madrid, Spain

    Juan Carlos Gutiérrez, Francisco Amaro & Ana Martín-González

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  1. Juan Carlos Gutiérrez
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  2. Francisco Amaro
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  3. Ana Martín-González
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Correspondence to Juan Carlos Gutiérrez .

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Editors and Affiliations

  1. Equipe Environnement et Microbiologie, IPREM UMR 5254, Université de Pau et des Pays de l’Adour, Pau, France

    Cristiana Cravo-Laureau

  2. Equipe Environnement et Microbiologie, IPREM UMR 5254, Université de Pau et des Pays de l’Adour, Pau, France

    Christine Cagnon

  3. Equipe Environnement et Microbiologie, IPREM UMR 5254, Université de Pau et des Pays de l’Adour, Pau, France

    Béatrice Lauga

  4. Equipe Environnement et Microbiologie, IPREM UMR 5254, Université de Pau et des Pays de l’Adour, Pau, France

    Robert Duran

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Gutiérrez, J.C., Amaro, F., Martín-González, A. (2017). Microbial Biosensors for Metal(loid)s. In: Cravo-Laureau, C., Cagnon, C., Lauga, B., Duran, R. (eds) Microbial Ecotoxicology. Springer, Cham. https://doi.org/10.1007/978-3-319-61795-4_13

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