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Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy

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Biobanking and Cryopreservation of Stem Cells

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 951))

Abstract

Adipose-Derived Stromal/Stem Cells (ASC) have considerable potential for regenerative medicine due to their abilities to proliferate, differentiate into multiple cell lineages, high cell yield, relative ease of acquisition, and almost no ethical concerns since they are derived from adult tissue. Storage of ASC by cryopreservation has been well described that maintains high cell yield and viability, stable immunophenotype, and robust differentiation potential post-thaw. This ability is crucial for banking research and for clinical therapeutic purposes that avoid the morbidity related to repetitive liposuction tissue harvests. ASC secrete various biomolecules such as cytokines which are reported to have immunomodulatory properties and therapeutic potential to reverse symptoms of multiple degenerative diseases/disorders. Nevertheless, safety regarding the use of these cells clinically is still under investigation. This chapter focuses on the different aspects of cryopreserved ASC and the methods to evaluate their functionality for future clinical use.

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Abbreviations

ASC:

Adipose-derived stromal/stem cells

ANGPT1:

Angiopoietin 1

CAL:

Cell Assisted Lipotranfer

CPA:

Cryoprotectant agents

cGMP:

Current Good Manufacturing Practice

DS:

Dextran sulfate

DMSO:

Dimethyl sulfoxide

EG:

Ethylene glycol

EAE:

Experimental autoimmune encephalitis

FDA:

U.S. Food and Drug Administration

GLUT4:

Glucose transporter

HGF:

Hepatocyte growth factor

HES:

Hydroxyethylstarch

INSR:

Insulin receptor

IL-1β:

Interleukin 1 beta

IL-6:

Interleukin 6

IL-12:

Interleukin 12

IFN-γ:

Interferon gamma

MSC:

Mesenchymal stem cell

SVF:

Stromal vascular fraction

MC:

Methylcellulose

PD:

Parkinson’s disease

PVP:

Polyvinylpyrollidone

TNF-α:

Tumor necrosis factor alpha

VEGF:

Vascular endothelial growth factor

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Disclosures

Fabiana Zanata received financial support from CAPES, Brazil (Process BEX 1524/15-1). Jeffrey Gimble and Xiying Wu are co-owners and employees of LaCell LLC, a biotechnology company focusing on research and clinical translation involving ASC and SVF cells.

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Authors and Affiliations

  1. Federal University of Sao Paulo, Sao Paulo, SP, Brazil

    Fabiana Zanata & Lydia Masako Ferreira

  2. Center for Stem Cell Research & Regenerative Medicine, Tulane University, New Orleans, LA, USA

    Fabiana Zanata & Jeffrey M. Gimble

  3. Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA

    Shahensha Shaik & Ram V. Devireddy

  4. La Cell LLC, New Orleans BioInnovation Center, Suite 304, 1441 Canal Street, New Orleans, LA, 70112, USA

    Xiying Wu & Jeffrey M. Gimble

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  1. Fabiana Zanata
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  2. Shahensha Shaik
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  3. Ram V. Devireddy
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  4. Xiying Wu
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  5. Lydia Masako Ferreira
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  6. Jeffrey M. Gimble
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Correspondence to Jeffrey M. Gimble .

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Editors and Affiliations

  1. Department of Oncology, University of Alberta, Edmonton, Alberta, Canada

    Feridoun Karimi-Busheri

  2. Department of Oncology, Cross Cancer Institute and University of Alberta, Edmonton, Alberta, Canada

    Michael Weinfeld

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Zanata, F., Shaik, S., Devireddy, R.V., Wu, X., Ferreira, L.M., Gimble, J.M. (2016). Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy. In: Karimi-Busheri, F., Weinfeld, M. (eds) Biobanking and Cryopreservation of Stem Cells. Advances in Experimental Medicine and Biology, vol 951. Springer, Cham. https://doi.org/10.1007/978-3-319-45457-3_11

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