Abstract
We developed a novel miscanthus cultivar, Soranovskii, based on Far Eastern populations that were successfully cultivated in West Siberia. Its plastid DNA belongs to M. sacchariflorus. We analyzed the saccharification of Soranovskii biomass compared with other species: Phalaris arundinacea, Thrachomitum lancifolium, and Sida hermaphrodita. The biomass of Phalaris was the most easily hydrolyzed. Milling and pretreatment by alkaline peroxide provided optimal conditions for complete enzymatic hydrolysis of miscanthus biomass. As the result, we obtained a sugar-containing substrate suitable for subsequent fermentation by microorganisms to ethanol and other substances. We also attempted to isolate cellulosolytic microorganisms from natural communities and to assess their biotechnological potential. Among the studied microorganisms we detected potential producers of ethanol and lactic acid on miscanthus.
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Acknowledgements
This study was supported by the Budget project VI.58.1.3 and by the EU FP7 Grant 150204 “Enhancing biomass production from marginal lands with perennial grasses.”
Cellobiase F10 and cellulase B1 from Pen. verruculosum were kindly provided by A. P. Sinitsyn.
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Goryachkovskaya, T. et al. (2016). “Soranovskii”: A New Miscanthus Cultivar Developed in Russia. In: Barth, S., Murphy-Bokern, D., Kalinina, O., Taylor, G., Jones, M. (eds) Perennial Biomass Crops for a Resource-Constrained World. Springer, Cham. https://doi.org/10.1007/978-3-319-44530-4_6
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DOI: https://doi.org/10.1007/978-3-319-44530-4_6
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