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Stratum Corneum Histopathology

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Measuring the Skin

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Abstract

Stratum corneum (SC) forms an efficient multifunctional barrier at the top of the skin. Thanks to its constant renewal and ingenious composition, SC can adapt to the changing environmental conditions. Because the SC barrier function is essential for the organism’s survival, most of the qualitative imperfections of the barrier tend to be compensated by the quantitative changes leading to overexpression of the horny layer. Such pathological situations, when unraveled, permit a better understanding of the fundamental mechanisms involved in the regulation of skin barrier function.

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References

  • Akiyama M, Sugiyama-Nakagiri Y, Sakai K, McMillan JR, Goto M, Arita K, Tsuji-Abe Y, Tabata N, Matsuoka K, Sasaki R, Sawamura D, Shimizu H. Mutations in lipid transporter ABCA12 in harlequin ichthyosis and functional recovery by corrective gene transfer. J Clin Invest. 2005;115(7):1777–84.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Baker H, Blair CP. Cell replacement in the human stratum corneum in old age. Br J Dermatol. 1968;80:367–72.

    Article  CAS  PubMed  Google Scholar 

  • Bouwstra JA, de Graaff A, Gooris GS, Nijsse J, Wiechers J, van Aelst AC. Water distribution and related morphology in human stratum corneum at different hydration levels. J Invest Dermatol. 2003a;120:750–8.

    Article  CAS  PubMed  Google Scholar 

  • Bouwstra JA, Honeywell-Nguyen PL, Gooris GS, Ponec M. Structure of the skin barrier and its modulation by vesicular formulations. Prog Lipid Res. 2003b;42:1–36.

    Article  CAS  PubMed  Google Scholar 

  • Candi E, Schmidt R, Melino G. The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol. 2005;6:328–40.

    Article  CAS  PubMed  Google Scholar 

  • Chapman SJ, Walsh A, Jackson SM, Friedmann PS. Lipids, proteins and corneocyte adhesion. Arch Dermatol Res. 1991;283:167–73.

    Article  CAS  PubMed  Google Scholar 

  • Chavanas S, Bodemer C, Rochat A, Hamel-Teillac D, Ali M, Irvine AD, Bonafé JL, Wilkinson J, Taïeb A, Barrandon Y, Harper JI, de Prost Y, Hovnanian A. Mutations in SPINK5, encoding a serine protease inhibitor, cause Netherton syndrome. Nat Genet. 2000;25(2):141–2.

    Article  CAS  PubMed  Google Scholar 

  • de Fine Olivarius F, Wulf HC, Crosby J, Norval M. The sunscreening effect of urocanic acid. Photodermatol Photoimmunol Photomed. 1996;12(3):95–9.

    Article  Google Scholar 

  • Denecker G, Ovaere P, Vandenabeele P, Declercq W. Caspase-14 reveals its secrets. J Cell Biol. 2008;180(3):451–8. doi:10.1083/jcb.200709098.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Eckhart L, Declercq W, Ban J, Rendl M, Lengauer B, Mayer C, Lippens S, Vandenabeele P, Tschachler E. Terminal differentiation of human keratinocytes and stratum corneum formation is associated with caspase-14 activation. J Invest Dermatol. 2000;115(6):1148–51.

    Article  CAS  PubMed  Google Scholar 

  • Elias PM. Defensive functions of the stratum corneum: integrative aspects. In: Elias PM, Feingold KR, editors. Skin barrier. New York: Taylor & Francis; 2006. p. 5–14.

    Google Scholar 

  • Elias PM, Menon GK. Structural and lipid biochemical correlates of the epidermal permeability barrier. Adv Lipid Res. 1991;24:1–26.

    CAS  PubMed  Google Scholar 

  • Elias PM, Fartasch M, Crumrine D, Behne M, Uchida Y, Holleran WM. Origin of the corneocyte lipid envelope (CLE): observations in harlequin ichthyosis and cultured human keratinocytes. J Invest Dermatol. 2000;115(4):765–9.

    Article  CAS  PubMed  Google Scholar 

  • Elias PM, Ahn SK, Denda M, Brown BE, Crumrine D, Kimutai LK, Kömüves L, Lee SH, Feingold KR. Modulations in epidermal calcium regulate the expression of differentiation-specific markers. J Invest Dermatol. 2002;119(5):1128–36.

    Article  CAS  PubMed  Google Scholar 

  • Elias PM, Crumrine D, Paller A, Rodriguez-Martin M, Williams ML. Pathogenesis of the cutaneous phenotype in inherited disorders of cholesterol metabolism: therapeutic implications for topical treatment of these disorders. Dermatoendocrinology. 2011;3(2):100–6. doi:10.4161/derm.3.2.14831.

    Article  CAS  Google Scholar 

  • Grove G, Kligman A. Age-associated changes in human epidermal cell renewal. J Gerontol. 1983;38:137–42.

    Article  CAS  PubMed  Google Scholar 

  • Hadj-Rabia S, Baala L, Vabres P, Hamel-Teillac D, Jacquemin E, Fabre M, Lyonnet S, De Prost Y, Munnich A, Hadchouel M, Smahi A. Claudin-1 gene mutations in neonatal sclerosing cholangitis associated with ichthyosis: a tight junction disease. Gastroenterology. 2004;127(5):1386–90. Erratum in: Gastroenterology. 2005;128(2):524.

    Article  CAS  PubMed  Google Scholar 

  • Haftek M, Serre G, Mils V, Thivolet J. Immunocytochemical evidence for a possible role of cross-linked keratinocyte envelopes in stratum corneum cohesion. J Histochem Cytochem. 1991;39(11):1531–8.

    Article  CAS  PubMed  Google Scholar 

  • Haftek M, Cambazard F, Dhouailly D, Réano A, Lachaux A, Simon M, Serre G, Claudy A, Schmitt D. A longitudinal study of an harlequin infant evolving clinically towards non-bullous congenital ichthyotic erythroderma. Br J Dermatol. 1996;135:448–53.

    Article  CAS  PubMed  Google Scholar 

  • Haftek M, Simon M, Kanitakis J, Maréchal S, Claudy A, Serre G, Schmitt D. Expression of corneodesmosin in the granular layer and stratum corneum of normal and diseased epidermis. Br J Dermatol. 1997;137:864–73.

    Article  CAS  PubMed  Google Scholar 

  • Haftek M, Teillon MH, Schmitt D. Stratum corneum, corneodesmosomes and ex vivo percutaneous penetration. Microsc Res Tech. 1998;43(3):242–9. doi:10.1002/(SICI)1097029(19981101)43:3<242::AID-JEMT6>3.0.CO;2-G.

    Article  CAS  PubMed  Google Scholar 

  • Haftek M, Simon M, Serre G. Corneodesmosomes: pivotal actors in the stratum corneum cohesion and desquamation. In: Elias PM, Feingold KR, editors. Skin barrier. New York: Taylor & Francis; 2006. p. 171–90.

    Google Scholar 

  • Haftek M, Callejon S, Sandjeu Y, Padois K, Falson F, Pirot F, Portes P, Demarne F, Jannin V. Compartmentalization of the human stratum corneum by persistent tight junction -like structures. Exp Dermatol. 2011;20:617–21. doi:10.1111/j.1600-0625.2011.01315.x.

    Article  CAS  PubMed  Google Scholar 

  • Haftek M, Callejon S, Pirot F, Traupe H, Oji V. Ultrastructural evaluation of the stratum corneum in peeling skin disease suggests a compensatory tight junction upregulation. J Invest Dermatol. 2012;132:S75–88. doi:10.1038/jid.2012.301 (abstract).

    Google Scholar 

  • Haftek M, Abdayem R, Colomb E, Hadj-Rabia S. Tight junction (TJ)-like structures contribute to the compensatory hyperkeratosis in claudin-1 –deficient patient with Ichthyosis, Hypotrychosis and Sclerosing Cholangitis (IHSC) syndrome (Abstr.). J Invest Dermatol. 2013;134(4):1172–5.

    Google Scholar 

  • Harding CR, Scott IR. Histidine-rich proteins (filaggrins). Structural and functional heterogeneity during epidermal differentiation. J Mol Biol. 1983;170:651–73.

    Article  CAS  PubMed  Google Scholar 

  • Henry J, Hsu CY, Haftek M, Nachat R, de Koning HD, Gardinal-Galera I, Hitomi K, Balica S, Jean-Decoster C, Schmitt AM, Paul C, Serre G, Simon M. Hornerin is a component of the epidermal cornified cell envelopes. FASEB J. 2011;25(5):1567–76. doi:10.1096/fj.10-168658.

    Article  CAS  PubMed  Google Scholar 

  • Hoath SB, Leahy DG. Formation and function of the stratum corneum. In: Marks R, editor. The essential stratum corneum. London: Martin Dunitz; 2002. p. 31–40.

    Chapter  Google Scholar 

  • Huber M, Rettler I, Bernasconi K, Frenk E, Lavrijsen SP, Ponec M, Bon A, Lautenschlager S, Schorderet DF, Hohl D. Mutations of keratinocyte transglutaminase in lamellar ichthyosis. Science. 1995;267(5197):525–8.

    Article  CAS  PubMed  Google Scholar 

  • Ishida-Yamamoto A, Deraison C, Bonnart C, Bitoun E, Robinson R, O’Brien TJ, Wakamatsu K, Ohtsubo S, Takahashi H, Hashimoto Y, Dopping-Hepenstal PJ, McGrath JA, Iizuka H, Richard G, Hovnanian A. LEKTI is localized in lamellar granules, separated from KLK5 and KLK7, and is secreted in the extracellular spaces of the superficial stratum granulosum. J Invest Dermatol. 2005;124(2):360–6.

    Article  CAS  PubMed  Google Scholar 

  • Iwai I, Han H, den Hollander L, Svensson S, Ofverstedt LG, Anwar J, Brewer J, Bloksgaard M, Laloeuf A, Nosek D, Masich S, Bagatolli LA, Skoglund U, Norlén L. The human skin barrier is organized as stacked bilayers of fully extended ceramides with cholesterol molecules associated with the ceramide sphingoid moiety. J Invest Dermatol. 2012;132(9):2215–25. doi:10.1038/jid.2012.43.

    Article  CAS  PubMed  Google Scholar 

  • Johannesson A, Hammar H. Measurement of the horny layer turnover after staining with dansyl chloride: description of a new method. Acta Derm Venereol. 1978;58:76–9.

    CAS  PubMed  Google Scholar 

  • Kalinin AE, Kajava AV, Steinert PM. Epithelial barrier function: assembly and structural features of the cornified cell envelope. Bioessays. 2002;24(9):789–800.

    Article  CAS  PubMed  Google Scholar 

  • Kamata Y, Taniguchi A, Yamamoto M, Nomura J, Ishihara K, Takahara H, Hibino T, Takeda A. Neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids. J Biol Chem. 2009;284(19):12829–36. doi:10.1074/jbc.M807908200.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kanitakis J, Lora V, Chouvet B, Zambruno G, Haftek M, Faure M. Circumscribed palmo-plantar hypokeratosis: a disease of desquamation? Immunohistological study of five cases and literature review. J Eur Acad Dermatol Venereol. 2011;25(3):296–301. doi:10.1111/j.1468-3083.2010.03784.x.

    Article  CAS  PubMed  Google Scholar 

  • Komatsu N, Suga Y, Saijoh K, Liu AC, Khan S, Mizuno Y, et al. Elevated human tissue kallikrein levels in the stratum corneum and serum of peeling skin syndrome-type B patients suggests an over-desquamation of corneocytes. J Invest Dermatol. 2006;126:2338–42.

    Article  CAS  PubMed  Google Scholar 

  • Krengel S, Geilen CC, Orfanos CE, Schaumburg-Lever GM. Histopathology and electron microscopy of psoriasis. In: Roenigk HH, Maibach HI, editors. Psoriasis. New York: Marcel Dekker; 1998. p. 409–17.

    Google Scholar 

  • Maestrini E, Korge BP, Ocana-Sierra J, Calzolari E, Cambiaghi S, Scudder PM, et al. A missense mutation in connexin26, D66H, causes mutilating keratoderma with sensorineural deafness (Vohwinkel’s syndrome) in three unrelated families. Hum Mol Genet. 1999;8:1237–43.

    Article  CAS  PubMed  Google Scholar 

  • Marekov LN, Steinert PM. Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope. J Biol Chem. 1998;273(28):17763–70.

    Article  CAS  PubMed  Google Scholar 

  • Markova NG, Marekov LN, Chipev CC, Gan SQ, Idler WW, Steinert PM. Profilaggrin is a major epidermal calcium-binding protein. Mol Cell Biol. 1993;13(1):613–25.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • McLean WH, Irvine AD. Heritable filaggrin disorders: the paradigm of atopic dermatitis. J Invest Dermatol. 2012;132(E1):E20–1. doi:10.1038/skinbio.2012.6.

    Article  PubMed  Google Scholar 

  • Méchin MC, Coudane F, Adoue V, Arnaud J, Duplan H, Charveron M, Schmitt AM, Takahara H, Serre G, Simon M. Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases. Cell Mol Life Sci. 2010;67(9):1491–503. doi:10.1007/s00018-010-0262-5.

    Article  PubMed  Google Scholar 

  • Menon GK, Price LF, Bommannan B, Elias PM, Feingold KR. Selective obliteration of the epidermal calcium gradient leads to enhanced lamellar body secretion. J Invest Dermatol. 1994;102(5):789–95.

    Article  CAS  PubMed  Google Scholar 

  • Michel S, Schmidt R, Shroot B, Reichert U. Morphological and biochemical characterization of the cornified envelopes from human epidermal keratinocytes of different origin. J Invest Dermatol. 1988;91(1):11–5.

    Article  CAS  PubMed  Google Scholar 

  • Mildner M, Jin J, Eckhart L, Kezic S, Gruber F, Barresi C, Stremnitzer C, Buchberger M, Mlitz V, Ballaun C, Sterniczky B, Födinger D, Tschachler E. Knockdown of filaggrin impairs diffusion barrier function and increases UV sensitivity in a human skin model. J Invest Dermatol. 2010;130(9):2286–94. doi:10.1038/jid.2010.115.

    Article  CAS  PubMed  Google Scholar 

  • Nemes Z, Marekov LN, Fésüs L, Steinert PM. A novel function for transglutaminase 1: attachment of long-chain omega-hydroxyceramides to involucrin by ester bond formation. Proc Natl Acad Sci U S A. 1999;96(15):8402–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Oji V, Tadini G, Akiyama M, Blanchet Bardon C, Bodemer C, Bourrat E, et al. Revised nomenclature and classification of inherited ichthyoses: results of the First Ichthyosis Consensus Conference in Sorèze. J Am Acad Dermatol. 2010a;63:607–41. doi:10.1016/j.jaad.2009.11.020.

    Article  PubMed  Google Scholar 

  • Oji V, Eckl KM, Aufenvenne K, Nätebus M, Tarinski T, Ackermann K, Seller N, Metze D, Nürnberg G, Fölster-Holst R, Schäfer-Korting M, Hausser I, Traupe H, Hennies HC. Loss of corneodesmosin leads to severe skin barrier defect, pruritus, and atopy: unraveling the peeling skin disease. Am J Hum Genet. 2010b;87(2):274–81. doi:10.1016/j.ajhg.2010.07.005.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Osawa R, Akiyama M, Izumi K, Ujiie H, Sakai K, Nemoto-Hasebe I, Yanagi T, Koizumi H, Shimizu H. Extremely severe palmoplantar hyperkeratosis in a generalized epidermolytic hyperkeratosis patient with a keratin 1 gene mutation. J Am Acad Dermatol. 2011;64(5):991–3. doi:10.1016/j.jaad.2009.04.019.

    Article  CAS  PubMed  Google Scholar 

  • Proksch E, Holleran WM, Menon GK, Elias PM, Feingold KR. Barrier function regulates epidermal lipid and DNA synthesis. Br J Dermatol. 1993;128(5):473–82.

    Article  CAS  PubMed  Google Scholar 

  • Rassner U, Feingold KR, Crumrine DA, Elias PM. Coordinate assembly of lipids and enzyme proteins into epidermal lamellar bodies. Tissue Cell. 1999;31(5):489–98.

    Article  CAS  PubMed  Google Scholar 

  • Rawlings AV, Harding CR. Moisturization and skin barrier function. Dermatol Ther. 2004;17 Suppl 1:43–8.

    Article  PubMed  Google Scholar 

  • Richard G, Brown N, Rouan F, Van der Schroeff JG, Bijlsma E, Eichenfield LF, et al. Genetic heterogeneity in erythrokeratodermia variabilis: novel mutations in the connexin gene GJB4 (Cx30.3) and genotype-phenotype correlations. J Invest Dermatol. 2003;120:601–9.

    Article  CAS  PubMed  Google Scholar 

  • Richter T, Peuckert C, Sattler M, Koenig K, Riemann I, Hintze U, Wittern KP, Wiesendanger R, Wepf R. Dead but highly dynamic – the stratum corneum is divided into three hydration zones. Skin Pharmacol Physiol. 2004;17(5):246–57.

    Article  CAS  PubMed  Google Scholar 

  • Schmuth M, Fluhr JW, Crumrine DC, Uchida Y, Hachem JP, Behne M, Moskowitz DG, Christiano AM, Feingold KR, Elias PM. Structural and functional consequences of loricrin mutations in human loricrin keratoderma (Vohwinkel syndrome with ichthyosis). J Invest Dermatol. 2004;122(4):909–22.

    Article  CAS  PubMed  Google Scholar 

  • Serre G, Mils V, Haftek M, Vincent C, Croute F, Réano A, Ouhayoun JP, Bettinger S, Soleilhavoup JP. Identification of late differentiation antigens of human cornified epithelia, expressed in re-organized desmosomes and bound to cross-linked envelope. J Invest Dermatol. 1991;97(6):1061–72.

    Article  CAS  PubMed  Google Scholar 

  • Simon M, Haftek M, Sebbag M, Montézin M, Girbal-Neuhauser E, Schmitt D, Serre G. Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis. Biochem J. 1996;317(Pt 1):173–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Simon M, Bernard D, Minondo AM, Camus C, Fiat F, Corcuff P, Schmidt R, Serre G. Persistence of both peripheral and non-peripheral corneodesmosomes in the upper stratum corneum of winter xerosis skin versus only peripheral in normal skin. J Invest Dermatol. 2001;116(1):23–30. doi:10.1046/j.1523-1747.2001.00208.x.

    Article  CAS  PubMed  Google Scholar 

  • Steinert PM, Marekov LN. Initiation of assembly of the cell envelope barrier structure of stratified squamous epithelia. Mol Biol Cell. 1999;10(12):4247–61.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Voegli R, Rawlings AV. Desquamation: it is almost all about proteases. In: Lodén M, Maibach HI, editors. Treatment of dry skin syndrome. Berlin: Springer-Verlag; 2012. p. 149–78. doi:10.1007/978-3-642-27606-4_11.

    Chapter  Google Scholar 

  • Wertz PW, Swartzendruber DC, Kitko DJ, Madison KC, Downing DT. The role of the corneocyte lipid envelopes in cohesion of the stratum corneum. J Invest Dermatol. 1989;93:169–72.

    Article  CAS  PubMed  Google Scholar 

  • Zettersten E, Man MQ, Sato J, Denda M, Farrell A, Ghadially R, Williams ML, Feingold KR, Elias PM. Recessive X-linked ichthyosis: role of cholesterol-sulfate accumulation in the barrier abnormality. J Invest Dermatol. 1998;111:784–90.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Recherche Dermatologique, EA 4169, Faculté de Médecine et de Pharmacie, Université Claude Bernard Lyon 1, 8, Av. Rockefeller, 69373, Lyon, France

    Marek Haftek

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  1. Marek Haftek
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Correspondence to Marek Haftek .

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Editors and Affiliations

  1. Medecine Vasculaire Lab. Ingénierie et de Biologie Cutanées, CHU Besancon Service Dermatologie et, Besancon, France

    Philippe Humbert

  2. San Francisco, California, USA

    Howard Maibach

  3. Department of Dermatology, Saint Jacques University Hospital, Besançon, France

    Ferial Fanian

  4. Univ. Besançon Dépt. Dermatologie, Besançon, France

    Pierre Agache

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Haftek, M. (2015). Stratum Corneum Histopathology. In: Humbert, P., Maibach, H., Fanian, F., Agache, P. (eds) Measuring the Skin. Springer, Cham. https://doi.org/10.1007/978-3-319-26594-0_23-1

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  • DOI: https://doi.org/10.1007/978-3-319-26594-0_23-1

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