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Fluorescence Detection Techniques

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Book cover Introduction to Fluorescence Sensing

Abstract

In this Chapter we concentrate on the methods of fluorescence observation. Starting from introducing the definitions and explaining fundamentals in fluorescence spectroscopy, the discussion proceeds to different methods of fluorescence that are valuable for sensing technologies. We discuss intensity-based sensing with and without intrinsic reference and the methods that do not need such reference and require the measurements of anisotropy and lifetime. The methods based on excimer and exciplex formation and Förster resonance energy transfer (FRET) involve interactions in the system of fluorescence reporters. In contrast, the sensing response based on wavelength-shifting and two-band wavelength-ratiometry relies on modulation of weak intermolecular interactions of a single fluorophore with its environment. The final section “Sensing and thinking” discusses the optimal choice of fluorescence detection techniques with the list of questions and problems addressed to the reader.

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  1. Laboratory of Nanobiotechnology Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Alexander P. Demchenko

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  1. Alexander P. Demchenko
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© 2015 Springer International Publishing Switzerland

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Demchenko, A.P. (2015). Fluorescence Detection Techniques. In: Introduction to Fluorescence Sensing. Springer, Cham. https://doi.org/10.1007/978-3-319-20780-3_3

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