Abstract
In order to be analyzed, the signal from fluorescence reporter should be first generated by optical excitation and then detected by designed instrumentation. In this Chapter we concentrate on different types of instruments that are used in fluorescence sensing. Some of them are universal (e.g. steady-state and time-resolved spectofluorimeters) and others (e.g. spotted microarrays and their readers) are specialized on obtaining very limited information on a large number of samples simultaneously. Depending on the purpose, different light sources and detectors can be selected, and the general trend is to decrease in power voltage and achieve miniaturization. Optical waveguides and optodes become increasingly popular. For sensing multiple analytes the choice between spotted and suspension microarrays can be made in view of different instrumentation required. We overview microfluidic devices that may couple sensor response with different reagent supply and chemical transformations and may host as biosensors the whole living cells. Optimizing convenience, sensitivity and precision for obtaining the proper sensor is the subject that will be finally discussed.
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Demchenko, A.P. (2015). The Sensing Devices. In: Introduction to Fluorescence Sensing. Springer, Cham. https://doi.org/10.1007/978-3-319-20780-3_11
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