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Protein and DNA Electrochemical Sensing Using Anodized Aluminum Oxide Nanochannel Arrays

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Nanoporous Alumina

Part of the book series: Springer Series in Materials Science ((SSMATERIALS,volume 219))

Abstract

This chapter shows the recent trends on the use of anodized aluminum oxide (AAO) nanochannel arrays for electrochemical sensing of proteins and DNA with a special focus on those based on voltammetric detections. Some general considerations on nanochannels and especially on AAO nanoporous membranes are given first, followed by the receptors (antibody, DNA) immobilization as well as the set-up configuration. The typical optimization procedures as well as the detection principles ranging from the use of ionic electroactive indicators and nanoparticles (used also as blockers) are discussed. Aspects related to the analytical performance of the developed devices while applied in diagnostics including cancer biomarker detection are also given. Finally an overview for future improvements and applications of this technology are included.

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Acknowledgments

We acknowledge support of the Spanish MINECO under Project MAT2011–25870 and through the Severo Ochoa Centers of Excellence Program under Grant SEV-2013-0295.

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Authors and Affiliations

  1. ICN2—Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193, Bellaterra (Barcelona), Spain

    Alfredo de la Escosura-Muñiz, Marisol Espinoza-Castañeda & Arben Merkoçi

  2. ICREA, Institucio Catalana de Recerca i Estudis Avançats, 08010, Barcelona, Spain

    Arben Merkoçi

Authors
  1. Alfredo de la Escosura-Muñiz
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  2. Marisol Espinoza-Castañeda
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  3. Arben Merkoçi
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Corresponding author

Correspondence to Arben Merkoçi .

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Editors and Affiliations

  1. School of Chemical Engineering, The University of Adelaide, Adelaide, South Australia, Australia

    Dusan Losic

  2. School of Chemical Engineering, The University of Adelaide, Adelaide, South Australia, Australia

    Abel Santos

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de la Escosura-Muñiz, A., Espinoza-Castañeda, M., Merkoçi, A. (2015). Protein and DNA Electrochemical Sensing Using Anodized Aluminum Oxide Nanochannel Arrays. In: Losic, D., Santos, A. (eds) Nanoporous Alumina. Springer Series in Materials Science, vol 219. Springer, Cham. https://doi.org/10.1007/978-3-319-20334-8_9

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