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REMI in Molecular Fungal Biology

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Genetic Transformation Systems in Fungi, Volume 1

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Restriction enzyme mediated integration (REMI) consists of a PEG or electroporation based transformation protocol in the presence of a restriction enzyme. In general, REMI results in increased transformation efficiency and more single locus integrations. This has made REMI a valuable tool for gene tagging and functional genomics in fungi. The mechanism of REMI, its use, and its alternatives are discussed.

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Notes

  1. 1.

    A detailed protocol on TAIL-PCR can be found in Chap. 46 of this volume

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Authors and Affiliations

  1. Department of Microbiology, Utrecht University, Utrecht, The Netherlands

    Aurin M. Vos, Luis G. Lugones Ph.D. & Han A. B. Wösten Ph.D.

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  1. Aurin M. Vos
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  2. Luis G. Lugones Ph.D.
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  3. Han A. B. Wösten Ph.D.
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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Vos, A.M., Lugones, L.G., Wösten, H.A.B. (2015). REMI in Molecular Fungal Biology. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_26

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