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Applications and Uses of Haploids

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Progress and Opportunities of Doubled Haploid Production

Part of the book series: SpringerBriefs in Plant Science ((BRIEFSPLANT,volume 6))

Abstract

The use of doubled haploidy has a great impact on plant breeding programs throughout the world especially to achieve food security on sustainable basis, which is a serious concern not only to developing countries but also to developed nations. There is a sharp increase in in-vitro studies to improve DH production and methodology/protocol (Table 5.1). Germana (2011) reported that 50 % of the barley cultivars currently grown in Europe have been developed by means of DH technologies. According to Faostat (2010), Canada is world’s 2nd biggest wheat exporter after USA, producing around 23 metric tons wheat every year. In Canada, wheat has been classified into nine different classes, out of which Canada Western Red Spring (CWRS) is the biggest class. It has been reported that during 2007, 3 out of 5 most grown cultivars of CWRS were developed through doubled haploid technology. Furthermore, cultivar “AC Andrew” is being grown on an area of 99 % under Canada Western Soft White Spring (CWSWS) class and it was also developed via anther culture (Dunwell 2010). Mapping populations are also being developed through DH that offers great potential for genetic studies, molecular analysis, and to map DNA markers. It was estimated that around 290 cultivars grown in various parts of the world during 2005 were developed through DH techniques (http://www.scri.ac.uk/assoc/COST851/DHTable2005.xls). Emerging uses of DH technology in plant breeding and crop improvement programs have been discussed as follows.

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References

  • Ahmad I, Day JP, MacDonald MV, Ingram DS (1991) Haploid culture and UV mutagenesis in rapid-cycling Brassica napus for the generation of resistance to chlorsulfuron and Alternaria brassicicola. Ann Bot 67(6):519–521

    Google Scholar 

  • Alavijeh MK, Ebadi A, Omidi M (2012) Embryogenic callus production in four grapevine (Vitis vinifera L.) cultivars using anther and whole flower culture. Iran J Hortic Sci 43(1):1–11

    Google Scholar 

  • Bagheri N, Babaeian-Jelodar N, Ghanbari A (2009) Evaluation of effective factors in anther culture of Iranian rice (Oryza sativa L.) cultivars. Biharean Biol 3(2):119–124

    Google Scholar 

  • Barakat MN, Al-Doss AA, Elshafei AA, Moustafa KA, Ahmed EI (2012) Anther culture response in wheat (Triticum aestivum L.) genotypes with HMW alleles. Cereal Res Commun 40(4):583–591. doi:10.1556/crc.40.2012.0011

    Article  CAS  Google Scholar 

  • Barbulescu DM, Burton WA, Salisbury PA (2011) Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived Brassica napus embryos. In Vitro Cell Dev Biol Plant 47(2):282–288. doi:10.1007/s11627-011-9353-8

    Article  Google Scholar 

  • Barro F, Fernandez-Escobar J, De La Vega M, Martin A (2001) Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores. Plant Breed 120(3):262–264. doi:10.1046/j.1439-0523.2001.00602.x

    Article  CAS  Google Scholar 

  • Basay S, Seniz V, Ellialtioglu S (2011) Obtaining dihaploid lines by using anther culture in the different eggplant cultivars. J Food Agric Environ 9(2):188–190

    Google Scholar 

  • Belinskaya EV (2010) Genotypic features of morphogenesis in spring barley anther culture. Cytol Genet 44(2):103–107. doi:10.3103/s0095452710020052

    Article  Google Scholar 

  • Belmonte M, Elhiti M, Waldner B, Stasolla C (2010) Depletion of cellular brassinolide decreases embryo production and disrupts the architecture of the apical meristems in Brassica napus microspore-derived embryos. J Exp Bot 61(10):2779–2794. doi:10.1093/jxb/erq110

    Article  PubMed  CAS  Google Scholar 

  • Belmonte M, Elhiti M, Ashihara H, Stasolla C (2011) Brassinolide-improved development of Brassica napus microspore-derived embryos is associated with increased activities of purine and pyrimidine salvage pathways. Planta 233(1):95–107. doi:10.1007/s00425-010-1287-6

    Article  PubMed  CAS  Google Scholar 

  • Benelli C, Germana MA, Ganino T, Beghe D, Fabbri A (2010) Morphological and anatomical observations of abnormal somatic embryos from anther cultures of Citrus reticulata. Biol Plant 54(2):224–230. doi:10.1007/s10535-010-0040-0

    Article  Google Scholar 

  • Berber M, Yildiz M, Abak K (2012) In: Litz RE, Folta KM, Talon M, Pliego Alfaro F (eds) Effects of irradiation doses on haploid embryo and plant production in naked and shelled seed pumpkins. Acta Hortic 929:381–384

    Google Scholar 

  • Bhat JG, Murthy HN (2008) Haploid plant regeneration from unpollinated ovule cultures of niger (Guizotia abyssinica (L. f.) cass.). Russ J Plant Physiol 55(2):241–245. doi:10.1134/s1021443708020118

    Article  CAS  Google Scholar 

  • Bhowmik P, Dirpaul J, Polowick P, Ferrie AMR (2011) A high throughput Brassica napus microspore culture system: influence of percoll gradient separation and bud selection on embryogenesis. Plant Cell Tiss Org 106(2):359–362. doi:10.1007/s11240-010-9913-3

    Article  CAS  Google Scholar 

  • Bilynska OV, Dulnyev PG (2012) Peculiarities of morphogenesis in spring barley anther culture in vitro on the nutrient media containing chemically modified starches. Fiziologiya i Biokhimia Kulturnykh Rastenii 44(5):440–448

    Google Scholar 

  • Bjornstad A, Skinnes H, Thoresen K (1993) Comparisons between doubled haploid lines produced by anther culture, the Hordeum bulbosum-method and lines produced by single seed descent in barley crosses. Euphytica 66(1–2):135–144. doi:10.1007/bf00023518

    Article  Google Scholar 

  • Bobkov SV (2010) Isolated pea anther culture. Russ Agric Sci 36(6):413–416. doi:10.3103/s1068367410060078

    Article  Google Scholar 

  • Bouatrous Y, Elhady EAAA, Djekoun A, Yekhlef N (2010) Production of haploid green plants by intergeneric crossing of Triticum durum Desf * Zea mays L. Am Eurasian J Agric Environ Sci 7(5):512–517

    Google Scholar 

  • Broughton S (2008) Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.). Plant Cell Tissue Organ Cult 95(2):185–195. doi:10.1007/s11240-008-9432-7

    Article  Google Scholar 

  • Broughton S (2011) The application of n-butanol improves embryo and green plant production in anther culture of Australian wheat (Triticum aestivum L.) genotypes. Crop Pasture Sci 62(10):813–822. doi:10.1071/cp11204

    Article  CAS  Google Scholar 

  • Burbulis N, Blinstrubiene A (2011) Genotypic and exogenous factors affecting linseed (Linum usitatissimum L.) anther culture. J Food Agric Environ 9(3–4):364–367

    Google Scholar 

  • Burbulis N, Blinstrubiene A, Kupriene R, Zilenaite L (2009) Effect of genotype and medium composition on flax (Linum usitatissimum L.) anther culture. Agron Res 7(Spl. Iss. 1):204–209

    Google Scholar 

  • Burbulis N, Blinstrubiene A, Masiene R, Jonytiene V (2012) Influence of genotype, growth regulators and sucrose concentration on linseed (Linum usitatissimum L.) anther culture. J Food Agric Environ 10(3–4):764–767

    CAS  Google Scholar 

  • Castillo AM, Cistue L, Romagosa I, Valles M (2001) Low responsiveness of six-rowed genotypes to androgenesis in barley does not have a pleiotropic basis. Genome 44(5):936–940. doi:10.1139/gen-44-5-936

    Article  PubMed  CAS  Google Scholar 

  • Chaitali S, Singh RP (2011) Anther culture response in boro rice hybrids. Asian J Biotechnol 3(5):470–477. doi:10.3923/ajbkr.2011.470.477

    Article  Google Scholar 

  • Chauhan H, Khurana P (2011) Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics. Plant Biotechnol J 9(3):408–417. doi:10.1111/j.1467-7652.2010.00561.x

    Article  PubMed  CAS  Google Scholar 

  • Chen QF, Wang CL, Lu YM, Shen M, Afza R, Duren MV, Brunner H (2001) Anther culture in connection with induced mutations for rice improvement. Euphytica 120(3):401–408. doi:10.1023/a:1017518702176

    Article  Google Scholar 

  • Chen C, Liu R, Lei H, Liu S, Wang Y (2010a) Effect of low temperature pre-treatment on embryoid induction rate of tobacco anther culture. Guizhou Agric Sci 5:16–18

    Google Scholar 

  • Chen D, Li S, Xiang Y, Zhang Z, Peng Y (2010b) Preliminary studies on the effects of plant phytosulfokine PSK-alpha in rice anther culture. Southwest China J Agric Sci 23(5):1447–1450

    Google Scholar 

  • Chen S, Wang X, Tang H, Qian X (2011) Distant hybridization technology of wheat * maize ­system and evaluation of its application in haploid breeding. Mol Plant Breed 9(4):519–524

    Google Scholar 

  • Chu CG, Xu SS, Friesen TL, Faris JD (2008) Whole genome mapping in a wheat doubled haploid population using SSRs and TRAPs and the identification of QTL for agronomic traits. Mol Breed 22(2):251–266. doi:10.1007/s11032-008-9171-9

    Article  CAS  Google Scholar 

  • Chugh A, Amundsen E, Eudes F (2009) Translocation of cell-penetrating peptides and delivery of their cargoes in triticale microspores. Plant Cell Rep 28(5):801–810. doi:10.1007/s00299-009-0692-4

    Article  PubMed  CAS  Google Scholar 

  • Cistue L, Romagosa I, Batlle F, Echavarri B (2009) Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture. Plant Cell Rep 28(5):727–735. doi:10.1007/s00299-009-0690-6

    Article  PubMed  CAS  Google Scholar 

  • Clarke HJ, Kumari M, Khan TN, Siddique KHM (2011) Poorly formed chloroplasts are barriers to successful interspecific hybridization in chickpea following in vitro embryo rescue. Plant Cell Tissue Organ Cult 106(3):465–473. doi:10.1007/s11240-011-9944-4

    Article  CAS  Google Scholar 

  • Corral-Martinez P, Segui-Simarro JM (2012) Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica 187(1):47–61. doi:10.1007/s10681-012-0715-z

    Article  Google Scholar 

  • Courtois B (1993) Comparison of single seed descent and anther culture-derived lines of 3 single crosses of rice. Theor Appl Genet 85(5):625–631

    Article  Google Scholar 

  • Croser JS, Lulsdorf MM, Grewal RK, Usher KM, Siddique KHM (2011) Isolated microspore culture of chickpea (Cicer arietinum L.): induction of androgenesis and cytological analysis of early haploid divisions. In Vitro Cell Dev Biol Plant 47(3):357–368. doi:10.1007/s11627-011-9346-7

    Article  CAS  Google Scholar 

  • Cuthbert JL, Somers DJ, Brule-Babel AL, Brown PD, Crow GH (2008) Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.). Theor Appl Genet 117(4):595–608. doi:10.1007/s00122-008-0804-5

    Article  PubMed  CAS  Google Scholar 

  • Danci M, Tudor-Radu CM, Alda S, Danci O (2011) Effect of culture medium and cultivars on callus formation and plants regeneration from anthers and immature embryos culture of wheat (Triticum aestivum L.). J Hortic Sci Biotechnol 15(4):110–112

    Google Scholar 

  • Datta SK, Potrykus I (1989) Artificial seeds in barley: encapsulation of microspore-derived embryos. Theor Appl Genet 77(6):820–824. doi:10.1007/BF00268333

    Article  Google Scholar 

  • Datta SK, Schmid J (1996) Prospects of artificial seeds from microspore derived embryos of cereals. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 2. Kluwer Academic, Amsterdam, pp 351–363

    Google Scholar 

  • De Cesaro T, Baggio MI, Zanetti SA, Suzin M, Augustin L, Brammer SP, Iorczeski EJ, Milach SCK (2009) Haplodiploid androgenetic breeding in oat: genotypic variation in anther size and microspore development stage. Sci Agric 66(1):118–122

    Article  Google Scholar 

  • de Moraes AP, Bered F, de Carvalho FIF, Kaltchuk-Santos E (2008) Morphological markers for microspore developmental stage in maize. Braz Arch Biol Technol 51(5):911–916

    Article  Google Scholar 

  • Diao W-P, Jia Y-Y, Song H, Zhang X-Q, Lou Q-F, Chen J-F (2009) Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenetants using SSR markers. Sci Hortic 119(3):246–251. doi:10.1016/j.scienta.2008.08.016

    Article  CAS  Google Scholar 

  • Doi H, Yokoi S, Hikage T, Nishihara M, Tsutsumi K-i, Takahata Y (2011) Gynogenesis in gentians (Gentiana triflora, G. scabra): production of haploids and doubled haploids. Plant Cell Rep 30(6):1099–1106. doi:10.1007/s00299-011-1017-y

  • Dormann P, Hoffmannbenning S, Balbo I, Benning C (1995) Isolation and characterization of an arabidopsis mutant deficient in the thylakoid lipid digalactosyl diacylglycerol. Plant Cell 7(11):1801–1810. doi:10.2307/3870188

    PubMed  CAS  Google Scholar 

  • Dubas E, Wedzony M, Petrovska B, Salaj J, Zur I (2010) Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x triticosecale wittm.). Acta Biol Cracov Bot 52(1):73–86. doi:10.2478/v10182-010-0010-z

    Google Scholar 

  • Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8(4):377–424. doi:10.1111/j.1467-7652.2009.00498.x

    Article  PubMed  CAS  Google Scholar 

  • Dyulgerova B, Valcheva D, Dimova D (2010) Anther culture response in winter barley (Hordeum vulgare L.). Genet Breed 39(1–2):45–49

    Google Scholar 

  • Eder J, Chalyk S (2002) In vivo haploid induction in maize. Theor Appl Genet 104(4):703–708. doi:10.1007/s00122-001-0773-4

    Article  PubMed  CAS  Google Scholar 

  • El-Hennawy MA, Abdalla AF, Shafey SA, Al-Ashkar IM (2011) Production of doubled haploid wheat lines (Triticum aestivum L.) using anther culture technique. Ann Agric Sci 56(2):69–77

    Google Scholar 

  • Ellaltoglu S, Basay S, Kusvuran S (2012) Investigations on the pollen dimorphism and its relationship with anther culture in eggplant (Patlcanda Polen dimorfizmi ve anter kulturu iliskisinin incelenmesi.). TABAD Tarm Bilimleri Arastrma Dergisi 5(1):149–152

    Google Scholar 

  • Ercan N, Sensoy FA (2012) In: Geelen D (ed) Determination of the optimum microspore development stage and optimum culture medium in asparagus (Asparagus officinalis var. altilis L.) for anther culture. Acta Hortic 961:153–158

    Google Scholar 

  • Esteves P, Marchand S, Sangare M, Belzile F (2010) Effects of pretreatments and hormone regime on green plant production in barley isolated microspore culture. In Vitro Cell Dev Biol Anim 46:S112

    Google Scholar 

  • Eudes F, Chugh A (2009) An overview of triticale doubled haploids. Adv Haploid Prod Higher Plants. doi:10.1007/978-1-4020-8854-4_6

    Google Scholar 

  • Faostat (2010) http://faostat.fao.org

  • Fennell A, Hauptmann R (1992) Electroporation and PEG delivery of DNA into maize microspores. Plant Cell Rep 11(11):567–570. doi:10.1007/BF00233094

    Article  CAS  Google Scholar 

  • Folling L, Olesen A (2001) Transformation of wheat (Triticum aestivum L.) microspore-derived callus and microspores by particle bombardment. Plant Cell Rep 20(7):629–636. doi:10.1007/s002990100371

    Article  CAS  Google Scholar 

  • Forodi BR, Hassandokht M, Kashi A, Sepahvand N (2009) Influence of spermidine on haploid plant production in Iranian onion (Allium cepa L.) populations through in vitro culture. Hortic Environ Biotechnol 50(5):461–466

    CAS  Google Scholar 

  • Froelicher Y, Bassene J-B, Jedidi-Neji E, Dambier D, Morillon R, Bernardini G, Costantino G, Ollitrault P (2007) Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets. Plant Cell Rep 26(7):937–944. doi:10.1007/s00299-007-0314-y

    Article  PubMed  CAS  Google Scholar 

  • Geng X, Wei Z, Yao X, Zhao Y (2010) Anther culture of Medicago sativa L. and ploidy detection. Acta Agric Sin 18(5):714–718

    Google Scholar 

  • Germana MA (2011) Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep 30(5):839–857. doi:10.1007/s00299-011-1061-7

    Article  PubMed  CAS  Google Scholar 

  • Ghazanfari P, Abdollahi MR, Moieni A, Moosavi SS (2012) Effect of plant-derived smoke extract on in vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas) microspore-derived embryos. Int J Plant Prod 6(3):309–324

    CAS  Google Scholar 

  • Godbole M, Murthy HN (2012) Parthenogenetic haploid plants using gamma irradiated pollen in snapmelon (Cucumis melo var. momordica). Plant Cell Tissue Organ Cult 109(1):167–170. doi:10.1007/s11240-011-0066-9

    Article  Google Scholar 

  • Gorecka K, Krzyzanowska D, Kiszczak W, Kowalska U (2009) Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos. Acta Physiol Plant 31(6):1139–1145. doi:10.1007/s11738-009-0332-1

    Article  Google Scholar 

  • Gorecka K, Kowalska U, Krzyzanowska D, Kiszczak W (2010) Obtaining carrot (Daucus carota L.) plants in isolated microspore cultures. J Appl Genet 51(2):141–147. doi:10.1007/bf03195722

    Article  PubMed  CAS  Google Scholar 

  • Grouh MSH, Vandati K, Lotfi M, Hassani D, Biranvand NP (2011) Production of haploids in persian walnut through parthenogenesis induced by gamma-irradiated pollen. J Am Soc Hortic Sci 136(3):198–204

    CAS  Google Scholar 

  • Grozeva S, Rodeva V, Todorova V, Pandeva R (2009) Obtaining of pepper plants via anther culture. Genet Breed 38(3–4):25–31

    Google Scholar 

  • Gu J, Liu K, Li S, Tian Y, Yang H, Yang M (2008) Study on the in vitro culture of cut plants in wheat haploid embryo induction by a wheat * maize cross. Front Agric China 2(4):391–395. doi:10.1007/s11703-008-0070-y

    Article  Google Scholar 

  • Guerrand J, Euzen M, Courand D, Bodin M, Tanguy JL, Menard V (2012) Microscopic study of artichoke (Cynara scolymus L.) floral pieces in order to develop a haploid production technique. In: Bazinet C, Mabeau S (eds) VII International symposium on artichoke, cardoon and their wild relatives. Acta Hortic 942:165–169

    Google Scholar 

  • Hassawi DS, Abu-Mallouh SA, Al-Abbadi AA, Shatnawi MA (2012) Organelles genome stability of wheat plantlets produced by anther culture. Afr J Biotechnol 11(22):6018–6026

    CAS  Google Scholar 

  • He P, Li JZ, Zheng XW, Shen LS, Lu CF, Chen Y, Zhu LH (2001) Comparison of molecular linkage maps and agronomic trait loci between DH and RIL populations derived from the same rice cross. Crop Sci 41(4):1240–1246. doi:10.2135/cropsci2001.4141240x

    Article  CAS  Google Scholar 

  • Heidari B, Saeidi G, Tabatabaei BES, Suenaga K (2012) QTLs involved in plant height, peduncle length and heading date of wheat (Triticum aestivum L.). J Agric Sci Technol 14(5): 1093–1104

    Google Scholar 

  • Herath HMI, Bandara DC (2011) Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids. J Natl Sci Found Sri Lanka 39(2):149–154

    Google Scholar 

  • Houben A, Sanei M, Pickering R (2011) Barley doubled-haploid production by uniparental ­chromosome elimination. Plant Cell Tissue Organ Cult 104(3):321–327. doi:10.1007/s11240-010-9856-8

    Article  Google Scholar 

  • Huang B (1992) Genetic manipulation of microspores and microspore-derived embryos. In Vitro Cell Dev Biol Plant 28(2):53–58

    Article  Google Scholar 

  • Hyde PT, Earle ED, Mutschler MA (2012) Doubled haploid onion (Allium cepa L.) lines and their impact on hybrid performance. Hortic Sci 47(12):1690–1695

    Google Scholar 

  • Irikova T, Grozeva S, Popov P, Rodeva V, Todorovska E (2011a) In vitro response of pepper anther culture (Capsicum annuum L.) depending on genotype, nutrient medium and duration of cultivation. Biotechnol Biotechnol Equip 25(4):2604–2609. doi:10.5504/bbeq.2011.0090

    Article  CAS  Google Scholar 

  • Irikova T, Grozeva S, Rodeva V (2011b) Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiol Plant 33(5):1559–1570. doi:10.1007/s11738-011-0736-6

    Article  CAS  Google Scholar 

  • Islam SMS (2010a) The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Aust J Crop Sci 4(9):660–665

    CAS  Google Scholar 

  • Islam SMS (2010b) Effect of embryoids age, size and shape for improvement of regeneration efficiency from microspore-derived embryos in wheat (Triticum aestivum L.). Plant Omics 3(5):149–153

    Google Scholar 

  • Islam SMS (2010c) The role of drought stress on anther culture of wheat (Triticum aestivum L.). Plant Tissue Cult Biotechnol 20(1):55–61

    Google Scholar 

  • Jacquard C, Mazeyrat-Gourbeyre F, Devaux P, Boutilier K, Baillieul F, Clement C (2009a) Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression. Planta 229(2):393–402. doi:10.1007/s00425-008-0838-6

    Article  PubMed  CAS  Google Scholar 

  • Jacquard C, Nolin F, Hecart C, Grauda D, Rashal I, Dhondt-Cordelier S, Sangwan RS, Devaux P, Mazeyrat-Gourbeyre F, Clement C (2009b) Microspore embryogenesis and programmed cell death in barley: effects of copper on albinism in recalcitrant cultivars. Plant Cell Rep 28(9):1329–1339. doi:10.1007/s00299-009-0733-z

    Article  PubMed  CAS  Google Scholar 

  • Jaeger K, Bartok T, Oerdoeg V, Barnabas B (2010) Improvement of maize (Zea mays L.) anther culture responses by algae-derived natural substances. S Afr J Bot 76(3):511–516. doi:10.1016/j.sajb.2010.03.009

    Article  CAS  Google Scholar 

  • Jardinaud M-F, Souvré A, Alibert G (1993) Transient GUS gene expression in Brassica napus electroporated microspores. Plant Sci 93(1–2):177–184. doi:10.1016/0168-9452(93)90047-4

    Article  CAS  Google Scholar 

  • Jo MH, Ham IK, Park MY, Kim TI, Lim YP, Lee EM (2012) Seed production ability of doubled haploid plants through microspore culture in Chinese cabbage (Brassica rapa L. ssp pekinensis) introduced from China. Korean J Hortic Sci 30(5):573–578. doi:10.7235/hort.2012.12004

    Article  Google Scholar 

  • Kantartzi SK, Roupakias DG (2009) In vitro gynogenesis in cotton (Gossypium sp.). Plant Cell Tissue Organ Cult 96(1):53–57. doi:10.1007/s11240-008-9459-9

    Article  Google Scholar 

  • Kasha KJ, Simion E, Oro R, Yao QA, Hu TC, Carlson AR (2001) An improved in vitro technique for isolated microspore culture of barley. Euphytica 120(3):379–385. doi:10.1023/a:1017564100823

    Article  Google Scholar 

  • Kelm C, Ghaffary SMT, Bruelheide H, Roder MS, Miersch S, Weber WE, Kema GHJ, Saal B (2012) The genetic architecture of seedling resistance to Septoria tritici blotch in the winter wheat doubled-haploid population Solitar x Mazurka. Mol Breed 29(3):813–830. doi:10.1007/s11032-011-9592-8

    Article  Google Scholar 

  • Khan MA, Ahmad J (2011) In vitro wheat haploid embryo production by wheat x maize cross system under different environmental conditions. Pak J Agric Sci 48(1):49–53

    Google Scholar 

  • Kielkowska A, Adamus A (2010) In vitro culture of unfertilized ovules in carrot (Daucus carota L.). Plant Cell Tissue Organ Cult 102(3):309–319. doi:10.1007/s11240-010-9735-3

    Article  Google Scholar 

  • Kim J, Lee S-S (2012) Identification of monogenic dominant male sterility and its suppressor gene from an induced mutation using a Broccoli (Brassica oleracea var. italica) microspore culture. Hortic Environ Biotechnol 53(3):237–241. doi:10.1007/s13580-012-0091-6

    Article  CAS  Google Scholar 

  • Kim M, Park EJ, An D, Lee Y (2013) High-quality embryo production and plant regeneration using a two-step culture system in isolated microspore cultures of hot pepper (Capsicum annuum L.). Plant Cell Tissue Organ Cult 112(2):191–201. doi:10.1007/s11240-012-0222-x

    Article  CAS  Google Scholar 

  • Kiszczak W, Krzyzanowska D, Strycharczuk K, Kowalska U, Wolko B, Gorecka K (2011) Determination of ploidy and homozygosity of carrot plants obtained from anther cultures. Acta Physiol Plant 33(2):401–407. doi:10.1007/s11738-010-0559-x

    Article  Google Scholar 

  • Kiviharju EM (2009) Anther culture derived doubled haploids in oat. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer, Dordrecht, pp 171–178. doi:10.1007/978-1-4020-8854-4_14

    Chapter  Google Scholar 

  • Koleva-Gudeva L, Trajkova F, Dimeska G, Spasenoski M (2009) Androgenesis efficiency in anther culture of pepper (Capsicum annuum L.). In: Krasteva L, Panayotov N (eds) IV Balkan symposium on vegetables and potatoes. Acta Hortic 830:183–190

    Google Scholar 

  • Kott LS (1996) Production of mutants using the rapeseed doubled haploid system. In: Induced mutation and molecular techniques for crop improvement. IAEA/FAO Proceedings of an international symposium on the use of induced mutations and molecular techniques for crop improvement, Vienne, Austria, pp 505–515

    Google Scholar 

  • Kour A, Bhatt U, Grewal S, Singh NK, Khanna VK (2008) Effect of wheat and maize genotypes for wheat haploid production in wheat x maize crosses. Indian J Genet Plant Breed 68(2):201–203

    Google Scholar 

  • Krystyna G, Dorota K, Urszula K, Waldemar K (2010) The technology of obtaining of carrot homozygous plants using the anther cultures. In Vitro Cell Dev Biol Anim 46:S189

    Google Scholar 

  • Krzewska M, Czyczylo-Mysza I, Dubas E, Golebiowska-Pikania G, Golemiec E, Stojalowski S, Chrupek M, Zur I (2012) Quantitative trait loci associated with androgenic responsiveness in triticale (xTriticosecale Wittm.) anther culture. Plant Cell Rep 31(11):2099–2108. doi:10.1007/s00299-012-1320-2

    Article  PubMed  CAS  Google Scholar 

  • Kuchel H, Williams K, Langridge P, Eagles HA, Jefferies SP (2007a) Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction. Theor Appl Genet 115(7):1015–1027. doi:10.1007/s00122-007-0628-8

    Article  PubMed  CAS  Google Scholar 

  • Kuchel H, Williams KJ, Langridge P, Eagles HA, Jefferies SP (2007b) Genetic dissection of grain yield in bread wheat. I. QTL analysis. Theor Appl Genet 115(8):1029–1041. doi:10.1007/s00122-007-0629-7

    Article  PubMed  CAS  Google Scholar 

  • Lantos C, Juhasz AG, Somogyi G, Otvos K, Vagi P, Mihaly R, Kristof Z, Somogyi N, Pauk J (2009) Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-­culture with ovary tissues of pepper or wheat. Plant Cell Tissue Organ Cult 97(3):285–293. doi:10.1007/s11240-009-9527-9

    Article  Google Scholar 

  • Lantos C, Juhasz AG, Vagi P, Mihaly R, Kristof Z, Pauk J (2012) Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.). Plant Biotechnol Rep 6(2):123–132. doi:10.1007/s11816-011-0205-0

    Article  Google Scholar 

  • Lee S-S, Lee S-A, Yang J, Kim J (2011) Developing stable progenies of xBrassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture. Theor Appl Genet 122(5):885–891. doi:10.1007/s00122-010-1494-3

    Article  PubMed  Google Scholar 

  • Li S, Huang Y, Xiao Y, Zhang B (2012) An observe of “Anther Culture in Pepper (Capsicum annuum L.) in vitro”. China Vegetables (20):1–6

    Google Scholar 

  • Liu Y, Li L, Chen S (2009) Genetic analysis of double haploid population from maize bio-induced parthenogenesis. J China Agric Univ 14(1):56–60

    CAS  Google Scholar 

  • Liu B, Miao J, Wang W, Zhang Z, Yang Y, Zhang Y, Wu X (2010) Research progress in onion (Allium cepa L.) haploid culture. China Vegetables (6):8–13

    Google Scholar 

  • Ljevnaic B, Kondic-Spika A, Kobiljski B, Hristov N (2009) Cytological characteristics of regenerants obtained from anther culture of wheat (Triticum aestivum L.). In: Mihailovic D, Miloradov MV (eds) Environmental, health and humanity issues in the down Danubian region: multidisciplinary approaches. World Scientific Publishing, Singapore, doi:10.1142/9789812834409_0015

  • Lotfi M, Salehi S (2008) Detection of cucumber parthenogenic haploid embryos by floating the immature seeds in liquid medium. In: Cucurbitaceae 2008: Proceedings of the IXth Eucarpia meeting on genetics and breeding of Cucurbitaceae

    Google Scholar 

  • Ma J, Zhang B (2008) Effects of glutamine, proline, and exogenous hormones on the anther culture of alfalfa. Acta Agric Sin 16(4):364–369

    CAS  Google Scholar 

  • Ma ZQ, Steffenson BJ, Prom LK, Lapitan NLV (2000) Mapping of quantitative trait loci for Fusarium head blight resistance in barley. Phytopathology 90(10):1079–1088. doi:10.1094/phyto.2000.90.10.1079

    Article  PubMed  CAS  Google Scholar 

  • Malik MR, Krochko JE (2009) Gene expression profiling of microspore embryogenesis in Brassica napus. Adv Haploid Prod Higher Plants. doi:10.1007/978-1-4020-8854-4_8

    Google Scholar 

  • Maluszynski M, Ahloowalia BS (2000) Mutation techniques in plant breeding. In: Moriaty M, Edington M, Mothersill C, Ward JF, Seymour C, Fry RJM (eds) Radiation research, vol 2. Cong Proceed, pp 251–254

    Google Scholar 

  • Mankowska G, Rutkowska-Krause I, Luwanska A, Wielgus K, Makowiecka J (2011) Use of anther culture to improve flax (Linum usitatissimum L.) resistance to Fusarium wilt (Wykorzystanie kultury pylnikowej w celu polepszenia odpornosci na fuzarioze lnu woknistego (Linum ustatissimum L.)). Biuletyn Instytutu Hodowli i Aklimatyzacji Roslin (260/261):333–339

    Google Scholar 

  • Marcinska I, Nowakowska A, Skrzypek E, Czyczylo-Mysza I (2013) Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.). Cent Eur J Biol 8(3):306–313. doi:10.2478/s11535-013-0132-2

    Article  CAS  Google Scholar 

  • Miki B, Huang B, Bird S, Kemble R, Simmonds D, Keller W (1989) A procedure for the microinjection of plant cells and protoplasts. J Tissue Cult Methods 12(4):139–144. doi:10.1007/BF01404440

    Article  Google Scholar 

  • Mohammadi PP, Moieni A, Ebrahimi A, Javidfar F (2012) Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.). Plant Cell Tiss Org 108(2):251–256. doi:10.1007/s11240-011-0036-2

    Article  CAS  Google Scholar 

  • Motallebi-Azar A (2010) Androgenic response of tomato (Lycopersicon esculentum Mill.) lines and their hybrids to anther culture. Russ Agric Sci 36(4):250–258

    Article  Google Scholar 

  • Motallebi-Azar A, Panahandeh J (2010) Effects of colchicine and cold duration pretreatments on androgenesis responses of tomato (Lycopersicon esculentum Mill) via anther culture. Russ Agric Sci 36(5):338–341. doi:10.3103/s106836741005006x

    Article  Google Scholar 

  • Mozgova GV, Zaitseva OI, Lemesh VA (2012) Structural changes in chloroplast genome accompanying albinism in anther culture of wheat and triticale. Cereal Res Commun 40(4):467–475. doi:10.1556/crc.40.2012.0007

    Article  CAS  Google Scholar 

  • Na H, Park S, Hwang G, Yoon M-K, Chun C (2009) Medium, AgNO3, activated charcoal and NAA effects on microspore culture in Brassica rapa. Korean J Hortic Sci 27(4):657–661

    CAS  Google Scholar 

  • Na H, Hwang G, Kwak J-H, Yoon MK, Chun C (2011a) Microspore derived embryo formation and doubled haploid plant production in broccoli (Brassica oleracea L. var italica) according to nutritional and environmental conditions. Afr J Biotechnol 10(59):12535–12541. doi:10.5897/ajb10.1287

    CAS  Google Scholar 

  • Na H, Kwak J-H, Chun C (2011b) The effects of plant growth regulators, activated charcoal, and AgNO3 on microspore derived embryo formation in broccoli (Brassica oleracea L. var. italica). Hortic Environ Biotechnol 52(5):524–529. doi:10.1007/s13580-011-0034-7

    Article  CAS  Google Scholar 

  • Nelson MN, Mason AS, Castello M-C, Thomson L, Yan G, Cowling WA (2009a) Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. x Brassica carinata. Theor Appl Genet 119(5):953–953. doi:10.1007/s00122-009-1126-y

    Article  Google Scholar 

  • Nelson MN, Mason AS, Castello M-C, Thomson L, Yan G, Cowling WA (2009b) Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. x Brassica carinata Braun. Theor Appl Genet 119(3):497–505. doi:10.1007/s00122-009-1056-8

    Article  PubMed  Google Scholar 

  • Obert B, Uvackova L, Pret’ova A (2009) Maize doubled haploids via anther and microspore culture. In: Danforth AT (ed) Corn crop production: growth, fertilization and yield. Nova Science, Hauppauge, NY, pp 333–343

    Google Scholar 

  • Ochoa-Alejo N (2012) Anther culture of chili pepper (Capsicum spp.). Methods Mol Biol 877:227–231

    Article  PubMed  CAS  Google Scholar 

  • Olsen FL (1991) Isolation and cultivation of embryogenic microspores from barley (Hordeum vulgare L). Hereditas 115(3):255–266

    Article  PubMed  CAS  Google Scholar 

  • Olszewska D, Kisiala A, Nowaczyk P (2011) The assessment of doubled haploid lines obtained in pepper (Capsicum annuum L.) anther culture. Folia Hortic 23(2):93–99

    Google Scholar 

  • Pathirana R, Frew T, Hedderley D, Timmerman-Vaughan G, Morgan E (2011) Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora. Plant Cell Rep 30(6):1055–1065. doi:10.1007/s00299-011-1012-3

    Article  PubMed  CAS  Google Scholar 

  • Ponitka A, Slusarkiewicz-Jarzina A (2011) Production of spontaneous and induced doubled-­haploid lines of winter triticale obtained through anther culture (Otrzymywanie spontanicznych i indukowanych linii podwojonych haploidow pszenzyta ozimego z wykorzystaniem kultur pylnikowych.). Biuletyn Instytutu Hodowli i Aklimatyzacji Roslin (260/261):183–191

    Google Scholar 

  • Poon S, Heath RL, Clarke AE (2012) A chimeric arabinogalactan protein promotes somatic embryogenesis in cotton cell culture. Plant Physiol 160(2):684–695. doi:10.1104/pp.112.203075

    Article  PubMed  CAS  Google Scholar 

  • Prem D, Solis M-T, Barany I, Rodriguez-Sanz H, Risueno MC, Testillano PS (2012) A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol 12:127. doi:10.1186/1471-2229-12-127

    Article  PubMed  Google Scholar 

  • Prodanovic S, Matzk F, Zoric D (2008) Effect of dicamba on wheat haploid embryo development. Cereal Res Commun 36(1):43–51. doi:10.1556/crc36.2008.1.5

    Article  CAS  Google Scholar 

  • Rakha MT, Metwally EI, Moustafa SA, Etman AA, Dewir YH (2012) Evaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitro cultures. Aust J Crop Sci 6(1):23–30

    CAS  Google Scholar 

  • Redha A, Suleman P (2011) Effects of exogenous application of polyamines on wheat anther cultures. Plant Cell Tissue Organ Cult 105(3):345–353. doi:10.1007/s11240-010-9873-7

    Article  CAS  Google Scholar 

  • Redha A, Talaat A (2008) Improvement of green plant regeneration by manipulation of anther culture induction medium of hexaploid wheat. Plant Cell Tissue Organ Cult 92(2):141–146. doi:10.1007/s11240-007-9315-3

    Article  Google Scholar 

  • Ren JP, Wang XG, Yin J (2010) Dicamba and sugar effects on callus induction and plant regeneration from mature embryo culture of wheat. Agr Sci China 9(1):31–37. doi:10.1016/s1671-2927(09)60064-x

    Article  CAS  Google Scholar 

  • Resch T, Ankele E, Badur R, Reiss B, Herberle-Bors E, Touraev A (2009) Immature pollen as a target for gene targeting. In: Touraev A, Forster B, Jain SM (eds) Advances in haploid production in higher plants. Springer, Dordrecht, pp 307–317. doi:10.1007/978-1-4020-8854-4_25

    Chapter  Google Scholar 

  • Rodriguez-Serrano M, Barany I, Prem D, Coronado MJ, Risueno MC, Testillano PS (2012) NO, ROS, and cell death associated with caspase-like activity increase in stress-induced microspore embryogenesis of barley. J Exp Bot 63(5):2007–2024. doi:10.1093/jxb/err400

    Article  PubMed  CAS  Google Scholar 

  • Rosenbaumova R, Krahulcova A, Krahulec F (2012) The intriguing complexity of parthenogenesis inheritance in Pilosella rubra (Asteraceae, Lactuceae). Sex Plant Reprod 25(3):185–196. doi:10.1007/s00497-012-0190-7

    Article  PubMed  Google Scholar 

  • Salas P, Prohens J, Segui-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182(2):261–274. doi:10.1007/s10681-011-0490-2

    Article  CAS  Google Scholar 

  • Salas P, Rivas-Sendra A, Prohens J, Segui-Simarro JM (2012) Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184(2):235–250. doi:10.1007/s10681-011-0569-9

    Article  Google Scholar 

  • Santra M, Ankrah N, Santra DK, Kidwell KK (2012) An improved wheat microspore culture technique for the production of doubled haploid plants. Crop Sci 52(5):2314–2320. doi:10.2135/cropsci2012.03.0141

    Article  Google Scholar 

  • Sayem MA, Maniruzzaman M, Siddique SS, Al-Amin M (2010) In vitro shoot regeneration through anther culture of Brassica spp. Bangladesh J Agric Res 35(2):331–341. doi:10.3329/bjar.v35i2.5896

    Article  Google Scholar 

  • Segui-Simarro JM, Corral-Martinez P, Corredor E, Raska I, Testillano PS, Risueno MC (2011) A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L. J Plant Physiol 168(8):746–757. doi:10.1016/j.jplph.2010.10.014

    Article  PubMed  CAS  Google Scholar 

  • Shabana M, Mari SN, Mari AK, Gaddi NH (2010) Induction of callus through anther and ovule culture in upland cotton (Gossypium hirsutum L.). World Appl Sci J 8(Spl Issue):76–79

    Google Scholar 

  • Shere UB, Dhage SJ (2009) Studies on anther culture in tomato (Lycopersicon esculentum). Int J Plant Sci (Muzaffarnagar) 4(2):433–435

    Google Scholar 

  • Shirdelmoghanloo H, Moieni A, Mousavi A (2009) Effects of embryo induction media and pretreatments in isolated microspore culture of hexaploid wheat (Triticum aestivum L. cv. Falat). Afr J Biotechnol 8(22):6134–6140

    CAS  Google Scholar 

  • Sidhu PK, Davies PA (2009) Regeneration of fertile green plants from oat isolated microspore culture. Plant Cell Rep 28(4):571–577. doi:10.1007/s00299-009-0684-4

    Article  PubMed  CAS  Google Scholar 

  • Smykalova I, Horacek J, Kubosiova M, Smirous P, Soukup A, Gasmanova N, Griga M (2012) Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi L.). In Vitro Cell Dev Biol Plant 48(1):30–39. doi:10.1007/s11627-011-9386-z

    CAS  Google Scholar 

  • Song Y, Zhou S, Du X, Hua S, Wei P, Chen Y (2012) Study on the factors affecting the anther culture efficiency of wheat. J Northwest A&F Univ Nat Sci Ed 40(5):62–68

    CAS  Google Scholar 

  • Soriano M, Cistue L, Castillo AM (2008) Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture. Plant Cell Rep 27(5):805–811. doi:10.1007/s00299-007-0500-y

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Snape JW, Cadalen T, Charmet G, Nakata N, Bernard S, Bernard M (2000) Detection of QTLs for heading time and photoperiod response in wheat using a doubled-haploid population. Genome 43(3):487–494. doi:10.1139/gen-43-3-487

    Article  PubMed  CAS  Google Scholar 

  • Stöger E, Fink C, Pfosser M, Heberle-Bors E (1995) Plant transformation by particle bombardment of embryogenic pollen. Plant Cell Rep 14(5):273–278. doi:10.1007/BF00232027

    Article  Google Scholar 

  • Sun N, Shi P, Wei L, Cao Y, Huang R, Li S, Liang W (2009) Genetic control analysis of triticale anther culture response. J Triticeae Crops 29(3):374–379

    Google Scholar 

  • Supena EDJ, Custers JBM (2011) Refinement of shed-microspore culture protocol to increase normal embryos production in hot pepper (Capsicum annuum L.). Sci Hortic 130(4):769–774. doi:10.1016/j.scienta.2011.08.037

    Article  Google Scholar 

  • Suprunova T, Shmykova N (2008) In vitro induction of haploid plants in unpollinated ovules, anther and microspore culture of Cucumis sativus. In: Pitrat M (ed) Cucurbitaceae 2008: Proceedings of the sixth Eucarpia meeting on genetics and breeding of Cucurbitaceae. INRA, Avignon, pp 371–374

    Google Scholar 

  • Suzuki T, Sato M, Takeuchi T (2012) Evaluation of the effects of five QTL regions on Fusarium head blight resistance and agronomic traits in spring wheat (Triticum aestivum L.). Breed Sci 62(1):11–17. doi:10.1270/jsbbs.62.11

    Article  PubMed  CAS  Google Scholar 

  • Swanson EB, Herrgesell MJ, Arnoldo M, Sippell DW, Wong RSC (1989) Microspore mutagenesis and selection - canola plants with field tolerance to the imidazolinones. Theor Appl Genet 78(4):525–530. doi:10.1007/bf00290837

    Article  CAS  Google Scholar 

  • Szafranska K, Cvikrova M, Kowalska U, Gorecka K, Gorecki R, Martincova O, Janas KM (2011) Influence of copper ions on growth, lipid peroxidation, and proline and polyamines content in carrot rosettes obtained from anther culture. Acta Physiol Plant 33(3):851–859. doi:10.1007/s11738-010-0610-y

    Article  CAS  Google Scholar 

  • Takahashi Y, Yokoi S, Takahata Y (2011) Improvement of microspore culture method for multiple samples in Brassica. Breed Sci 61(1):96–98. doi:10.1270/jsbbs.61.96

    Article  Google Scholar 

  • Takahashi Y, Yokoi S, Takahata Y (2012) Effects of genotypes and culture conditions on microspore embryogenesis and plant regeneration in several subspecies of Brassica rapa L. Plant Biotechnol Rep 6(4):297–304. doi:10.1007/s11816-012-0224-5

    Article  Google Scholar 

  • Takahira J, Cousin A, Nelson MN, Cowling WA (2011) Improvement in efficiency of microspore culture to produce doubled haploid canola (Brassica napus L.) by flow cytometry. Plant Cell Tiss Org 104(1):51–59. doi:10.1007/s11240-010-9803-8

    Article  Google Scholar 

  • Tang Y, Li H, Liu J, Liu B (2009) Callus formation from anther culture in balsam pear (Momordica charantia L.). Am Eurasian J Agric Environ Sci 6(3):308–312

    CAS  Google Scholar 

  • Tang Y, Li X, Li J, Ma C, Lai J, Li H (2012) Effect of different pretreatment on callus formation from anther in balsam pear (Momordica charantia L.). J Med Plant Res 6(17):3393–3395. doi:10.5897/jmpr11.1752

    Google Scholar 

  • Targonska M, Hromada-Judycka A, Bolibok-Bragoszewska H, Rakoczy-Trojanowska M (2013) The specificity and genetic background of the rye (Secale cereale L.) tissue culture response. Plant Cell Rep 32(1):1–9. doi:10.1007/s00299-012-1342-9

    Article  PubMed  CAS  Google Scholar 

  • Taskin H, Buyukalaca S, Keles D, Ekbic E (2011) Induction of microspore-derived embryos by anther culture in selected pepper genotypes. Afr J Biotechnol 10(75):17116–17121. doi:10.5897/ajb11.2023

    CAS  Google Scholar 

  • Testillano PS, Coronado MJ, Thierry AM, Matthys-Rochon E, Risueno MC (2010) In situ detection of Esr proteins secretion during maize microspore embryogenesis and their secretion blockage show effects on the culture progression. Funct Plant Biol 37(10):985–994. doi:10.1071/fp10066

    Article  CAS  Google Scholar 

  • Tomaszewska-Sowa M (2010) Cytometric analyses of sugar beet (Beta vulgaris L.) plants regenerated from unfertilized ovules cultured in vitro. Electron J Pol Agric Univ 13(4):09-art 09

    Google Scholar 

  • Touraev A, Pfosser M, Heberle-Bors E (2001) The microspore: a haploid multipurpose cell. Adv Bot Res 35:53–109. doi:10.1016/s0065-2296(01)35004-8

    Article  Google Scholar 

  • Tyrnov VS, Smolkina YV (2011) The new technology of maize breeding by parthenogenesis. Maize Genet Coop News Lett 85:36

    Google Scholar 

  • Usha B, Khanna VK (2010) Pollen tube study in relation to haploid production in wheat maize crosses. Pantnagar J Res 8(1):39–42

    Google Scholar 

  • Vischi M, Marchetti S (1997) Strong extracellular nuclease activity displayed by barley (Hordeum vulgare L.) uninucleate microspores. Theor Appl Genet 95(1–2):185–190. doi:10.1007/s001220050546

    Article  CAS  Google Scholar 

  • Wan GL, Naeem MS, Geng XX, Xu L, Li B, Jilani G, Zhou WJ (2011) Optimization of microspore embryogenesis and plant regeneration protocols for Brassica napus. Int J Agric Biol 13(1):83–88

    CAS  Google Scholar 

  • Wang S, Liu Y, Khalid H, Wang Y, Shi Z, Lin F (2010) Genetic study on colchicine induction of parthenogenesis in maize. World Appl Sci J 10(2):143–146

    CAS  Google Scholar 

  • Wang Y, Tong Y, Li Y, Zhang Y, Zhang J, Feng J, Feng H (2011) High frequency plant regeneration from microspore-derived embryos of ornamental kale (Brassica oleracea L. var. acephala). Sci Hortic 130(1):296–302. doi:10.1016/j.scienta.2011.06.029

    Article  CAS  Google Scholar 

  • Wen J, Zeng X-h, Pu Y-y, Qi L-p, Li Z-y, Tu J-x, Ma C-z, Shen J-x, Fu T-d (2010) Meiotic nondisjunction in resynthesized Brassica napus and generation of aneuploids through microspore culture and their characterization. Euphytica 173(1):99–111. doi:10.1007/s10681-010-0129-8

    Article  Google Scholar 

  • Weber BN, Hamernik AJ, Jansky SH (2012) Hybridization barriers between diploid Solanum tuberosum and wild Solanum raphanifolium. Genet Resour Crop Evol 59(7):1287–1293. doi:10.1007/s10722-012-9883-x

    Article  Google Scholar 

  • Wei J, Chen M, Liu Z, Zhu L (2010) Identification method of maize haploid induced by parthenogenesis inducer. Chin Seed 1(1):40–42

    Google Scholar 

  • Weyen J, Orsini J, Gnad H (2012) A new wheat anther culture technology preventing albinism in the EU winter wheat gene pool. In Vitro Cell Dev Biol Anim 48:74

    Google Scholar 

  • Winarto B, da Silva JAT (2011) Microspore culture protocol for Indonesian Brassica oleracea. Plant Cell Tiss Org 107(2):305–315. doi:10.1007/s11240-011-9981-z

    Article  Google Scholar 

  • Yadollahi A, Abdollahi MR, Moieni A, Danaee M (2011) Effects of carbon source, polyethylene glycol and abscisic acid on secondary embryo induction and maturation in rapeseed (Brassica napus L.) microspore-derived embryos. Acta Physiol Plant 33(5):1905–1912. doi:10.1007/s11738-011-0738-4

    Article  CAS  Google Scholar 

  • Yahata M, Yasuda K, Nagasawa K, Harusaki S, Komatsu H, Kunitake H (2010) Production of haploid plant of ‘Banpeiyu’ Pummelo Citrus maxima (Burm.) Merr. by pollination with soft X-ray-irradiated pollen. J Jpn Soc Hortic Sci 79(3):239–245

    Article  CAS  Google Scholar 

  • Yamagishi M, Otani M, Higashi M, Fukuta Y, Fukui K, Yano M, Shimada T (1998) Chromosomal regions controlling anther culturability in rice (Oryza sativa L.). Euphytica 103(2):227–234. doi:10.1023/a:1018328708322

    Article  CAS  Google Scholar 

  • Yamashita K-i, Nakazawa Y, Namai K, Amagai M, Tsukazaki H, Wako T, Kojima A (2012) Modes of inheritance of two apomixis components, diplospory and parthenogenesis, in Chinese chive (Allium ramosum) revealed by analysis of the segregating population generated by back-­crossing between amphimictic and apomictic diploids. Breed Sci 62(2):160–169. doi:10.1270/jsbbs.62.160

    Article  PubMed  CAS  Google Scholar 

  • Yang Y, Yu C, Song H, Xu H, Xu Z (2011) Optimization of culture conditions of F 1 anther culture from different panicle shape japonica/indica hybrid rice. J Shenyang Agric Univ 42(3):354–357

    Google Scholar 

  • Yuan L, Song D, Gao G, He Y (2011) Improvement of resistance to rice blast in PGMS line Y58S by molecular marker-assisted selection and anther culture. Genomics Appl Biol 30(5):620–625

    Google Scholar 

  • Yuan SX, Su YB, Liu YM, Fang ZY, Yang LM, Zhuang M, Zhang YY, Sun PT (2012) Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage. Plant Cell Tissue Organ Cult 110(1):69–76. doi:10.1007/s11240-012-0131-z

    Article  CAS  Google Scholar 

  • Zeng X, Wen J, Wan Z, Yi B, Shen J, Ma C, Tu J, Fu T (2010) Effects of Bleomycin on microspore embryogenesis in Brassica napus and detection of somaclonal variation using AFLP molecular markers. Plant Cell Tiss Org 101(1):23–29. doi:10.1007/s11240-009-9658-z

    Article  CAS  Google Scholar 

  • Zhang L, Zhang L, Luo J, Chen W, Hao M, Liu B, Yan Z, Zhang B, Zhang H, Zheng Y, Liu D, Yen Y (2011) Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process. J Genet Genomics 38(2):89–94. doi:10.1016/j.jcg.2011.01.004

    Article  PubMed  Google Scholar 

  • Zhao J, Zhou X, Zhang Z, Yang B, Zhou S (2010) Effects of culture media on anther culture of chili pepper (Capsicum annuum L.). J Hunan Agric Univ 36(2):181–187

    Article  CAS  Google Scholar 

  • Zhao L, Liu L, Guo H, Gu J, Zhao S, Li J, XieYongDun (2012) Combining ability analysis of anther culture traits of three wheat genotypes with high regeneration ability. J Triticeae Crops 32(3):427–430

    CAS  Google Scholar 

  • Zur I, Dubas E, Golemiec E, Szechynska-Hebda M, Golebiowska G, Wedzony M (2009) Stress-­related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (x Triticosecale Wittm.). Plant Cell Rep 28(8):1279–1287. doi:10.1007/s00299-009-0730-2

    Article  PubMed  CAS  Google Scholar 

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  1. University of Alberta, Edmonton, AB, Canada

    Muhammad Asif

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  1. Muhammad Asif
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© 2013 Springer International Publishing Switzerland

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Asif, M. (2013). Applications and Uses of Haploids. In: Progress and Opportunities of Doubled Haploid Production. SpringerBriefs in Plant Science, vol 6. Springer, Heidelberg. https://doi.org/10.1007/978-3-319-00732-8_5

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