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Androgenesis: A Fascinating Doubled Haploid Production Process

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Progress and Opportunities of Doubled Haploid Production

Part of the book series: SpringerBriefs in Plant Science ((BRIEFSPLANT,volume 6))

Abstract

Androgenesis is one of the most important methods that have been extensively used in plant breeding programs to produce double haploids. It involves the induction of microspore embryogenesis that leads to the development of a haploid embryo instead of mature pollen grain. The microspore embryogenesis is usually brought about by modifying the environmental conditions of anthers/microspores by reprogramming their gametophytic pathway towards sporophytic growth and development. Under natural conditions, the microspore develops into a mature pollen grain that comprises of generative and vegetative nuclei. The generative nucleus develops into two sperm nuclei. Thus, the sporophytic development should be started before the onset of cell division when the gamete cells in the microspores are still totipotent. However, the embryogenic stage of microspores varies greatly among species (Touraev et al. 2001). The microspores are amenable to androgenesis and consist of haploid (n) number of chromosomes and therefore; give rise to haploid plants. Androgenesis can be divided into three distinguished steps (1) embryogenesis induction (2) regeneration of haploids followed by (3) artificial chromosome doubling. The production of haploids or doubled haploids (DH) via androgenesis can be achieved either through isolated microspore culture or anther culture.

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  1. University of Alberta, Edmonton, AB, Canada

    Muhammad Asif

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Asif, M. (2013). Androgenesis: A Fascinating Doubled Haploid Production Process. In: Progress and Opportunities of Doubled Haploid Production. SpringerBriefs in Plant Science, vol 6. Springer, Heidelberg. https://doi.org/10.1007/978-3-319-00732-8_2

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