Skip to main content
SpringerLink
Log in
Book cover

The Many Faces of Osteoarthritis pp 219–230Cite as

Birkhäuser

CD44 and cartilage matrix stabilization

  • Conference paper

Abstract

One prominent feature of osteoarthritic cartilage is an inherent failure to retain a proteoglycan-rich extracellular matrix. This lack of retention occurs even as the chondrocytes respond with episodes of pronounced matrix biosynthesis. Thus, osteoarthritis is likely manifested in part by the inability of chondrocytes to retain matrix at the cell surface. Another aspect of degenerative changes in osteoarthritis is enhanced catabolism, often termed chondrocytic chondrolysis. Although many facets of chondrocyte catabolism and attempted repair have been extensively investigated, the signals that initiate such metabolic changes remain unclear. Matrix receptors direct the assembly and retention of the pericellular matrix and provide a linkage to the cytoskeleton. CD44 is the principal receptor for hyaluronan (HA) expressed by chondrocytes [1–3]. The interactions between matrix components and receptors establish a cell-associated pool of extracellular matrix molecules. These associations have the potential to signal changes in cell behavior, such as proliferation, apoptosis and matrix biosynthesis or turnover. In addition to chondrocyte integrins [4],CD44 represents another class of receptors that can participate in these matrix-cell-cytoskeleton interactions [3,5].

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chow G, Knudson CB, Homandberg G, Knudson W (1995) Increased CD44 expression in bovine articular chondrocytes by catabolic cellular mediators. J Biol Chem 270: 27734–27741

    Article  PubMed  CAS  Google Scholar 

  2. Aguiar DJ, Knudson W, Knudson CB (1999) Internalization of the hyaluronan receptor CD44 by chondrocytes. Exp Cell Res 252: 292–302

    Article  PubMed  CAS  Google Scholar 

  3. Knudson W, Knudson CB (1999) Hyaluronan receptor, CD44. Glycoforumhttp://www.glycoforum.gr.jp/science/hyaluronan/HA10/HA10E.html

    Google Scholar 

  4. Loeser RF (2000) Chondrocyte integrin expression and function. Biorheology 37:109–116

    PubMed  CAS  Google Scholar 

  5. Knudson CB, Knudson W (1993) Hyaluronan-binding proteins in development, tissue homeostasis and disease. FASEB J 7: 1233–1241

    PubMed  CAS  Google Scholar 

  6. Hauselmann HJ, Aydelotte MB, Schumacher BL, Kuettner KE, Gitelis SH, Thonar EJ-MA (1992) Synthesis and turnover of proteoglycans by human and bovine adult articular chondrocytes cultured in alginate beads. Matrix 12: 130–136

    Google Scholar 

  7. Clarris BJ, Fraser JRE (1968) On the pericellular zone of some mammalian cells in vitro. Exp Cell Res 49: 181–193

    Article  PubMed  CAS  Google Scholar 

  8. Knudson CB (1993) Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix. j Cell Biol 120: 825–834

    Article  PubMed  CAS  Google Scholar 

  9. Knudson W, Aguiar DJ, Hua Q, Knudson CB (1996) CD44-anchored hyaluronan-rich pericellular matrices: An ultrastructural and biochemical analysis. Exp Cell Res 228: 216–228

    Article  PubMed  CAS  Google Scholar 

  10. Knudson W, Knudson CB (1991) Assembly of a chondrocyte-like pericellular matrix on non-chondrogenic cells. j Cell Sci 99: 227–235

    PubMed  Google Scholar 

  11. Knudson CB, Nofal GA, Pamintuan L, Aguiar DJ (1999) The chondrocyte pericellular matrix: a model for hyaluronan-mediated cell-matrix interactions. Biochem Soc Trans 27: 142–147

    PubMed  CAS  Google Scholar 

  12. Chow G, Nietfeld J, Knudson CB, Knudson W (1998) Antisense inhibition of chondrocyte CD44 expression results in cartilage chondrolysis. Arthritis Rheum 41: 1411–1419

    Article  PubMed  CAS  Google Scholar 

  13. Nishida Y, D’Souza AL, Thonar JMA, Knudson W (2000) 1L-1a stimulates hyaluronan metabolism in human articular cartilage. Arthritis Rheum 43: 1315–1326

    Article  PubMed  CAS  Google Scholar 

  14. Nishida Y, Knudson CB, Eger W, Kuettner KE, Knudson W (2000) Osteogenic protein-1 stimulates cell-associated matrix assembly by normal human articular chondrocytes. Arthritis Rheum 43: 206–214

    Article  PubMed  CAS  Google Scholar 

  15. Nishida Y, Knudson CB, Kuettner KE, Knudson W (2000) Osteogenic protein-1 promotes the synthesis and retention of extracellular matrix within bovine articular cartilage and chondrocyte cultures. Osteoarthritis Cartilage 8: 127–136

    Article  PubMed  CAS  Google Scholar 

  16. Ostergaard K, Salter DM, Andersen CB, Petersen J, Bendtzen K (1997) CD44 expression is up-regulated in the deep zone of osteoarthritic cartilage from human femoral heads. Histopathology 31: 451–459

    Article  PubMed  CAS  Google Scholar 

  17. Camp RL, Kraus TA, Pure E (1991) Variations in the cytoskeletal interaction and post-translational modification of the CD44 homing receptor in macrophages. J Cell Biol 115: 1283–1292

    Article  PubMed  CAS  Google Scholar 

  18. Brown PD, Benya PD (1988) Alterations in chondrocyte cytoskeletal architecture during phenotypic modulation by retinoic acid and dihydrocytochalasin B-induced reexpression. J Cell Biol 106: 171–179

    Article  PubMed  CAS  Google Scholar 

  19. Spector I, Shochet N, Blasberger D, Kashman Y (1989) Latrunculins-novel marine macrolides that disrupt microfilament organization and affect cell growth. Cell Motility Cytoskeleton 13: 127–144

    Article  CAS  Google Scholar 

  20. Hua Q, Knudson CB, Knudson W (1993) Internalization of hyaluronan by chondrocytes occurs via receptor-mediated endocytosis. J Cell Sci 106: 365–375

    PubMed  CAS  Google Scholar 

  21. Nofal GA, Knudson CB (2000) Cytoskeletal disruption by latrunculin A decreases chondrocyte matrix retention. Ortho Res Soc Trans 25: 1005

    Google Scholar 

  22. Knudson W, Casey B, Nishida Y, Eger W, Kucttncr KE, Knudson CB (2000) Hyaluronan oligosaccharides perturb cartilage matrix homeostasis and induce chondrocytic chondrolysis. Arthritis Rheum 43: 1165–1174

    Article  PubMed  CAS  Google Scholar 

  23. Lark MW, Bayne EK, Flanagan J, Harper CF, Hoerrner LA, Hutchinson NI, Singer II, Donatelli SA, Weidner JR, Williams HR, Mumford RA, Lohmander LS (1997) Aggrecan degradation in human cartilage. J Clin Invest 100: 93–105

    Article  PubMed  CAS  Google Scholar 

  24. Sastry SK, Burridge K (2000) Focal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics. Exp Cell Res 261: 25–36

    Article  PubMed  CAS  Google Scholar 

  25. Svoboda KKH (1998) Chondrocyte-matrix attachment complexes mediate survival and differentiation. Micro Res Tech 43: 111–122

    Article  CAS  Google Scholar 

  26. Nofal GA, Knudson CB (1999) Chondrocyte CD44 interacts with the cytoskeleton via ankyrin. Ortho Res Soc Trans 24: 130

    Google Scholar 

  27. Tsukita S, Oishi K, Sato N, Sagara J, Kawai A (1994) ERM family members as molec-ular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons. J Cell Biol 126: 391–401

    Article  PubMed  CAS  Google Scholar 

  28. Legg JW, Isacke CM (1998) Identification and functional analysis of ezrin-binding site in the hyaluronan receptor, CD44. Current Biol 8: 705–708

    Article  CAS  Google Scholar 

  29. Lokeshwar VB, Fregien N, Bourguignon LYW (1994) Ankyrin-binding domain of CD44(GP85) is required for the expression of hyaluronic acid-mediated adhesion function. J Cell Biol 126: 1099–1109

    Article  PubMed  CAS  Google Scholar 

  30. Embry J, Knudson W (2001) Hyaluronan and the hyaluronan binding region of aggrecan are co-internalized by articular chondrocytes. Orthop Res Soc Trans 26: 401

    Google Scholar 

  31. Naux D (1992) The structure of an endocytosis signal. Trends Cell Biol 2: 189–192

    Article  Google Scholar 

  32. Michaely P, Kamal A, Anderson RG, Bennett V (1999) A requirement for ankyrin binding to clathrin during coated pit budding. J Biol Chem 274: 35908–35913

    Article  PubMed  CAS  Google Scholar 

  33. Isacke CM (1994) The role of the cytoplasmic domain in regulating CD44 function. J Cell Sci 107: 2353–2359

    CAS  Google Scholar 

  34. Formby B, Stern R (1998) Phosphorylation stabilizes alternatively spliced CD44 mRNA transcripts in breast cancer cells: inhibition by antiscnse complementary to casein kinase II mRNA. Mol Cell Biochem 187: 23–31

    Article  CAS  Google Scholar 

  35. Lesley J, Hyman R (1998) CD44 structure and function. Frontiers Bioscience 3: 616–630

    Google Scholar 

  36. Rousche KT, Knudson CB (2001) Casein kinase: a potential mediator of CD44 phosphorylation in human articular cartilage. Ortho Res Soc Trans 26: 323

    Google Scholar 

  37. Muchleman C, Bareithcr D, Huch K, Cole AA, Kuettner KE (1997) Prevalence of degenerative morphological changes in the joints of the lower extremity. Osteoarthritis Cartilage 5: 23–37

    Article  Google Scholar 

  38. Screaton GR, Bell MV, Jackson DG, Cornelis FB, Gerth U, Bell JI (1992) Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons. Proc Natl Acad Sci USA 89: 12160–12164

    Article  PubMed  CAS  Google Scholar 

  39. Gunthert U, Hofmann M, Rudy W, Reber S, Zoller M, Haussmann I, Matzku S, Wenzel A, Ponta H, Herrlich P (1991) A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 65: 13–24

    Article  PubMed  CAS  Google Scholar 

  40. Sherman L, Wainwright D, Ponta H, Herrlich P (1998) A splice variant of CD44 expressed in the apical ectodermal ridge presents fibroblast growth factors to limb mesenchyme and is required for limb outgrowth. Genes Dey 12: 1058–1071

    Article  CAS  Google Scholar 

  41. Jiang H, Knudson CB, Knudson W (2001) Antisense inhibition of alternatively spliced CD44 variant in human articular chondrocytes promotes hyaluronan internalization. Arthritis Rheum 44: 2599–2610

    Article  PubMed  CAS  Google Scholar 

  42. Knudson W, Bartnik E, Knudson CB (1993) Assembly of pericellular matrices by COS-7 cells transfected with CD44 homing receptor genes. Proc Natl Acad Sci USA 90: 4003–4007

    Article  PubMed  CAS  Google Scholar 

  43. Jiang H, Peterson R, Wang W, Bartnik E, Knudson CB, Knudson W (2002) A requirement for the CD44 cytoplasmic domain for hyaluronan binding, pericellular matrix assembly and receptor-mediated endocytosis in COS-7 cells. J Biol Chem 277: 10531–10538

    Article  CAS  Google Scholar 

  44. Peterson R, Knudson CB, Knudson W (2001) Expression of CD44 isoforms and truncation mutants by articular chondrocytes. Trans Ortho Res Soc Trans 26: 395

    Google Scholar 

  45. Madry H, Trippel SB (2000) Efficient lipid-mediated gene transfer to articular chondrocytes. Gene Ther 7: 286–291

    Article  PubMed  CAS  Google Scholar 

  46. Bayliss MT (1992) Metabolism of animal and human osteoarthritis cartilage. In: KE Kuettner, R Schleyerbach, JG Peyron, VC Hascall (eds): Articular cartilage and osteoarthritis. Raven Press, New York, 487–500

    Google Scholar 

  47. Muir H (1995) The chondrocyte, architech of cartilage: Biomechanics, structure, function and molecular biology of cartilage matrix macromolecules. BioEssays 17: 1039–1048

    Article  PubMed  CAS  Google Scholar 

  48. Knudson CB, Knudson W (2001) Cartilage proteoglycans. Seminars Cell Dev Biol 12: 69–78

    Article  CAS  Google Scholar 

  49. Kuettner KE (1994) Osteoarthritis: Cartilage integrity and homeostasis. In: JH Klippel, PA Dieppe (eds): Rheumatology. Mosby-Year Book Europe Limited, St. Louis, MO, 6.1–6.16

    Google Scholar 

  50. Reddi AH (1998) Role of morphogenetic proteins in skeletal tissue engineering and regeneration. Nature Biotech 16: 247–252

    Article  CAS  Google Scholar 

  51. Daniels K, Solursh M (1991) Modulation of chondrogenesis by the cytoskeleton and extracellular matrix. J Cell Sci 100: 249–254

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Rush Medical College, 1653 W. Congress Parkway, Chicago, IL 60612, USA

    Cheryl B. Knudson, Kathleen T. Rousche, Richard S. Peterson, Geraldine Chow & Warren Knudson

Authors
  1. Cheryl B. Knudson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kathleen T. Rousche
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard S. Peterson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Geraldine Chow
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Warren Knudson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, The Cleveland Clinic Foundation, Orthopaedic Research Center, 9500 Euclid Avenue, Cleveland, OH, 44195, USA

    Vincent C. Hascall

  2. Department of Biochemistry, Rush Medical College at Rush Presbyterian-St. Luke’s Medical Center, 1653 West Congress Parkway, Chicago, IL, 60612-3864, USA

    Klaus E. Kuettner

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Basel AG

About this paper

Cite this paper

Knudson, C.B., Rousche, K.T., Peterson, R.S., Chow, G., Knudson, W. (2002). CD44 and cartilage matrix stabilization. In: Hascall, V.C., Kuettner, K.E. (eds) The Many Faces of Osteoarthritis. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8133-3_22

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-8133-3_22

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9450-0

  • Online ISBN: 978-3-0348-8133-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation