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Pectin Degrading Enzymes

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Pectin: Technological and Physiological Properties

Abstract

The properties of pectinolytic enzymes have drawn a great attention from many research teams as potential biological catalysts in diverse industrial processes for plant transformation. Pectin-related enzymes are ubiquitous, produced by plants, fungi and bacteria. Due to the highly complex structure described in Chap. 2, pectin is the substrate for many modifying and degrading enzymes belonging to three families of Carbohydrate Active enZymes (CAZy): glycoside hydrolases, polysaccharide lyases and carbohydrate esterases. They are distinguished by their different mechanisms (hydrolysis or β-elimination), action patterns (endo/exo) and specificities (homogalacturonan, rhamnogalacturonan, neutral side-chains, oligogalacturonates). In the 90s, literature dealt with their biochemical characterisation while more recently, progress in protein biotechnologies allowed investigating the production of recombinant and mutated enzymes to study the role of key amino acids. This chapter reviews the state of knowledge of pectin-processing enzymes to illustrate their structural and functional diversities. Their physico-chemical and biological properties are presented, as well as the occurrence of inhibiting proteins.

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  1. INRAE, UR BIA Biopolymères Interactions Assemblages, Nantes, France

    Estelle Bonnin

  2. BIOPI Biologie des Plantes et Innovation SFR Condorcet, Université de Picardie, Amiens, France

    Jérôme Pelloux

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  1. Estelle Bonnin
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Correspondence to Estelle Bonnin .

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  1. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, Australia

    Vassilis Kontogiorgos

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Bonnin, E., Pelloux, J. (2020). Pectin Degrading Enzymes. In: Kontogiorgos, V. (eds) Pectin: Technological and Physiological Properties. Springer, Cham. https://doi.org/10.1007/978-3-030-53421-9_3

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