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Effect of Bacterial Infection on the Expression of Stress Proteins and Antioxidative Enzymes in Japanese Flounder

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Evolution of Marine Coastal Ecosystems under the Pressure of Global Changes

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Abstract

Stress induced by environmental stimuli, such as pollutants and infection, in fish is thought to influence their fitness. Bacterium Edwardsiella tarda is a virulent intracellular pathogen of commercial fish species. Edwardsiellosis caused by E. tarda is one of the most severe diseases in Japanese flounder Paralichthys olivaceus. However, little is known about the effect of bacterial (such as E. tarda) infectious-induced stress on the expression of stress proteins and antioxidative enzymes in fish. In the present study, we examined the expression of heat shock protein (HSP) 70 and superoxide dismutases (SODs) in the hepatopancreas of Japanese flounder in response to E. tarda infection. HSP70 expression was rapidly increased and was significantly higher in fish 48 h after being infected with E. tarda, compared to that in control fish. The expression level of Cu, Zn–SOD in infected fish increased from 24 to 48 h and peaked at 48 h post-infection. On the other hand, the expression level of Mn–SOD in infected fish gradually increased from 12 h post-infection and remained high between 24 and 48 h post-infections. The changes in expression levels of HSP70 and SODs are suggestive of reactive oxygen species-induced oxidative stress in Japanese flounder. The results also suggest that both HSP70 and SODs play roles in mediating immune response upon E. tarda infection in Japanese flounder. We hypothesize that these proteins are important factors that protect cells against oxidative stress.

T. Nakano and K. Osatomi are the two main contributors.

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Acknowledgements

The authors wish to thank Dr. M. Sato at Tohoku University, Japan; Drs. T. Ishihara and K. Hara at Nagasaki University, Japan; Dr. G. K. Iwama at University of British Columbia/Quest University, Canada, for valuable discussion and suggestions. TN is also grateful to Dr. H.-J. Ceccaldi, Prof. Emeritus, at Ecole Pratique des Hautes Etudes, France, and Dr. Patrick Prouzet, IFREMER/Societe Franco-Japonaise d’Oceanographie, France, for giving an opportunity to present our research topics during 17th CFJO symposium (Coast Bordeaux 2017). Some researches performed by TN and KO were financially supported in part by a Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science (JSPS). TN was also supported in part by a fund to Core-to-Core Program (A. Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” from JSPS. Each author of this study further declares no relationships with the companies or manufacturers who may benefit from the results of the present study. The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. Marine Biochemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 980-8572, Japan

    Toshiki Nakano, Nanami Miura, Akiko Yamauchi, Toshiyasu Yamaguchi & Yoshihiro Ochiai

  2. Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki, 852-8521, Japan

    Kiyoshi Osatomi, Yoko Aikawa-Fukuda, Kinya Kanai & Asami Yoshida

  3. Nutrition Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 980-8572, Japan

    Hitoshi Shirakawa

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  1. Toshiki Nakano
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  2. Kiyoshi Osatomi
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  10. Yoshihiro Ochiai
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Correspondence to Toshiki Nakano .

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Editors and Affiliations

  1. Institut Océanographique, JFSO France Institut Océanographique, Marseille, France

    Hubert-Jean Ceccaldi

  2. JAMSTEC (JAPAN AGENCY FOR EARTH-OCEAN SCIENCE AND TECHNOLOGY) and JFSO France, Paris, France

    Yves Hénocque

  3. Yokohama College of Commerce, JFSO-Japan, Tokyo, Japan

    Teruhisa Komatsu

  4. JFSO France, ST JEAN DE LUZ, France

    Patrick Prouzet

  5. UMR CNRS 5805 EPOC-OASU, Pessac, France

    Benoit Sautour

  6. Centre for Marine Research and Operations, Tokyo University of Marine Science and Technology, Tokyo, Japan

    Jiro Yoshida

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Nakano, T. et al. (2020). Effect of Bacterial Infection on the Expression of Stress Proteins and Antioxidative Enzymes in Japanese Flounder. In: Ceccaldi, HJ., Hénocque, Y., Komatsu, T., Prouzet, P., Sautour, B., Yoshida, J. (eds) Evolution of Marine Coastal Ecosystems under the Pressure of Global Changes. Springer, Cham. https://doi.org/10.1007/978-3-030-43484-7_8

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