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Photocurable Alginate Bioink Development for Cartilage Replacement Bioprinting

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Progress in Digital and Physical Manufacturing (ProDPM 2019)

Part of the book series: Lecture Notes in Mechanical Engineering ((LNME))

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Abstract

Bioink design and assessment for tissue engineering replacement is a key topic of research. This article investigates suitable photocurable alginate bioink precursors for bioprinting and the fabrication of 3D constructs for cartilage replacements. Alginate chemically modified with methacrylate anhydride groups is considered and assessed using different techniques. 2% Alginate methacrylate (AlgMA) solutions containing different concentrations of methacrylate and different reaction times were investigated. Nuclear magnetic resonance (NMR) results show the ability to tune the unsaturation degree by changing the reaction conditions. Rheological characterization results show that all alginate methacrylate precursor solutions exhibit a shear thinning behavior. Biocompatibility and cytotoxicity results were no cytotoxicity was observed.

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References

  1. Buckwalter, J.A., Mankin, H.J.: Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. Instr. Course Lect. 47, 487–504 (1998)

    Google Scholar 

  2. Simon, T.M., Jackson, D.W.: Articular cartilage: injury pathways and treatment options. Sports Med. Arthroscopy Rev. 26(1), 31–39 (2018)

    Article  Google Scholar 

  3. Flik, K.R., Verma, N., Cole, B.J., Bach, B.R.: Articular cartilage. In: Williams, R.J. (ed.) Cartilage Repair Strategies, pp. 1–12. Humana Press, Totowa (2007)

    Google Scholar 

  4. Guo, T., Lembong, J., Zhang, L.G., Fisher, J.P.: Three-dimensional printing articular cartilage: recapitulating the complexity of native tissue. Tissue Eng. Part B: Rev. 23(3), 225–236 (2017)

    Article  Google Scholar 

  5. Nukavarapu, S.P., Dorcemus, D.L.: Osteochondral tissue engineering: current strategies and challenges. Biotechnol. Adv. 31(5), 706–721 (2013)

    Article  Google Scholar 

  6. Buckwalter, J.A.: Articular cartilage injuries. Clin. Orthop. Relat. Res.® 402, 21–37 (2002)

    Article  Google Scholar 

  7. Swieszkowski, W., Tuan, B.H.S., Kurzydlowski, K.J., Hutmacher, D.W.: Repair and regeneration of osteochondral defects in the articular joints. Biomol. Eng. 24(5), 489–495 (2007)

    Article  Google Scholar 

  8. Redman, S.N., Oldfield, S.F., Archer, C.W.: Current strategies for articular cartilage repair. Eur. Cell Mater. 9(23–32), 23–32 (2005)

    Article  Google Scholar 

  9. Fragonas, E., Valente, M., Pozzi-Mucelli, M., Toffanin, R., Rizzo, R., Silvestri, F., Vittur, F.: Articular cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate. Biomaterials 21(8), 795–801 (2000)

    Article  Google Scholar 

  10. Mishbak, H., Caetano, G.F., Pereira, R.F., Bartolo, P.J.: Photocurable Crosslinked Sodium Alginate and Gelatin based hydrogels for articular cartilage applications, p. 6 (2016)

    Google Scholar 

  11. Jeon, O., Bouhadir, K.H., Mansour, J.M., Alsberg, E.: Photocrosslinked alginate hydrogels with tunable biodegradation rates and mechanical properties. Biomaterials 30(14), 2724–2734 (2009)

    Article  Google Scholar 

  12. Smeds, K.A., Grinstaff, M.W.: Photocrosslinkable polysaccharides for in situ hydrogel formation. J. Biomed. Mater. Res. Off. J. Soc. Biomater. Jpn. Soc. Biomater 54(1), 115–121 (2001)

    Article  Google Scholar 

  13. Billiet, T., Van Gasse, B., Gevaert, E., Cornelissen, M., Martins, J.C., Dubruel, P.: Quantitative contrasts in the photopolymerization of acrylamide and methacrylamide-functionalized gelatin hydrogel building blocks. Macromol. Biosci. 13(11), 1531–1545 (2013)

    Article  Google Scholar 

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Acknowledgement

The authors greatly acknowledge the government of Iraq for their kind support Mr. Hussein Mishbak’s PhD through a grant provided by Higher Committee for Development Education Iraq (HCEDIRAQ). Mr. Aslan would like to acknowledge The Repoblic of Turkey Ministry of National Education for supporting his PhD through grant

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Authors and Affiliations

  1. Biomedical Engineering Department, School of Engineering, The University of Thi-Qar, Thi-Qar, Iraq

    H. Mishbak

  2. School of Mechanical, Aerospace and Civil Engineering, Manchester Biomanufacturing Centre, University of Manchester, Manchester, UK

    H. Mishbak, Enes Aslan, Glen Cooper & P. J. Bartolo

Authors
  1. H. Mishbak
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  2. Enes Aslan
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  3. Glen Cooper
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  4. P. J. Bartolo
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Corresponding author

Correspondence to H. Mishbak .

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Editors and Affiliations

  1. Polytechnic Institute of Leiria, Marinha Grande, Portugal

    Henrique A. Almeida

  2. Escola Superior de Tecnologia e Gestão, Leiria, Portugal

    Joel C. Vasco

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Mishbak, H., Aslan, E., Cooper, G., Bartolo, P.J. (2020). Photocurable Alginate Bioink Development for Cartilage Replacement Bioprinting. In: Almeida, H., Vasco, J. (eds) Progress in Digital and Physical Manufacturing. ProDPM 2019. Lecture Notes in Mechanical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-030-29041-2_30

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  • DOI: https://doi.org/10.1007/978-3-030-29041-2_30

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-29040-5

  • Online ISBN: 978-3-030-29041-2

  • eBook Packages: EngineeringEngineering (R0)

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