Abstract
Colloidal stability of nanoparticles in biological media is crucial to preserve their utility as aggregation often leads to undesirable biological response. A quantitative measurement of nanoparticles aggregation in solution would provide a valuable assessment of colloidal stability of bio–nano interfaces after surface functionalization. Here, we develop a quantitative technique based on optical absorption to assay the colloidal stability of plasmonic nanoparticles functionalized with different amphiphilic surface ligands in biologically relevant media.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Alkilany AM, Murphy CJ (2010) Toxicity and cellular uptake of gold nanoparticles: what we have learned so far? J Nanoparticle Res 12:2313–2333
Boldt K, Bruns OT, Gaponik N, Eychmuller A (2006) Comparative examination of the stability of semiconductor quantum dots in various biochemical buffers. J Phys Chem B 110:1959–1963
Noh M, Kim T, Lee H, Kim CK, Joo SW, Lee K (2010) Fluorescence quenching caused by aggregation of water-soluble CdSe quantum dots. Colloids Surf a-Physicochem Eng Aspects 359:39–44
Yang P, Murase N, Yu JH (2011) SiO(2) beads with quantum dots: preparation and stability investigation for bioapplications. Colloids Surf a-Physicochem Eng Aspects 385:159–165
de Puig H, Federici S, Baxamusa SH, Bergese P, Hamad-Schifferli K (2011) Quantifying the nanomachinery of the nanoparticle-biomolecule interface. Small 7:2477–2484
Ferhan AR, Guo LH, Kim DH (2010) Influence of ionic strength and surfactant concentration on electrostatic surfacial assembly of cetyltrimethylammonium bromide-capped gold nanorods on fully immersed glass. Langmuir 26:12433–12442
MacCuspie RI (2011) Colloidal stability of silver nanoparticles in biologically relevant conditions. J Nanoparticle Res 13:2893–2908
Sethi M, Joung G, Knecht MR (2009) Stability and electrostatic assembly of Au nanorods for Use in biological assays. Langmuir 25:317–325
Stebounova LV, Guio E, Grassian VH (2011) Silver nanoparticles in simulated biological media: a study of aggregation, sedimentation, and dissolution. J Nanoparticle Res 13:233–244
Chen ZP, Zhang Y, Xu K, Xu RZ, Liu JW, Gu N (2008) Stability of hydrophilic magnetic nanoparticles under biologically relevant conditions. J Nanosci Nanotechnol 8:6260–6265
Heister E, Neves V, Lamprecht C, Silva SRP, Coley HM, McFadden J (2012) Drug loading, dispersion stability, and therapeutic efficacy in targeted drug delivery with carbon nanotubes. Carbon 50:622–632
Santander-Ortega MJ, Peula-Garcia JM, Goycoolea FM, Ortega-Vinuesa JL (2011) Chitosan nanocapsules: Effect of chitosan molecular weight and acetylation degree on electrokinetic behaviour and colloidal stability. Colloids Surf B Biointerfaces 82:571–580
Park JY, Choi ES, Baek MJ, Lee GH (2009) Colloidal stability of amino acid coated magnetite nanoparticles in physiological fluid. Mater Lett 63:379–381
Fang C, Bhattarai N, Sun C, Zhang MQ (2009) Functionalized nanoparticles with long-term stability in biological media. Small 5:1637–1641
Lin YS, Abadeer N, Haynes CL (2011) Stability of small mesoporous silica nanoparticles in biological media. Chem Commun 47:532–534
Wiogo HTR, Lim M, Bulmus V, Yun J, Amal R (2011) Stabilization of magnetic iron oxide nanoparticles in biological media by fetal bovine serum (FBS). Langmuir 27:843–850
Cirtiu CM, Raychoudhury T, Ghoshal S, Moores A (2011) Systematic comparison of the size, surface characteristics and colloidal stability of zero valent iron nanoparticles pre- and post-grafted with common polymers. Colloids Surf a-Physicochem Eng Aspects 390:95–104
Kah JC-Y, Zubieta A, Saavedra RA, Hamad-Schifferli K (2012) Stability of gold nanorods passivated with amphiphilic ligands. Langmuir 28:8834–8844
Acknowledgments
This work was supported by the NSF grant DMR #0906838.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Kah, J.C.Y. (2013). Stability and Aggregation Assays of Nanoparticles in Biological Media. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_9
Download citation
DOI: https://doi.org/10.1007/978-1-62703-462-3_9
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-461-6
Online ISBN: 978-1-62703-462-3
eBook Packages: Springer Protocols