Abstract
Upon wounding or herbivory, plants quickly react by activating various defense mechanisms. A major part of these defenses is thought to be regulated by the jasmonate pathway through the induction of jasmonic acid and its biologically active jasmonoyl-isoleucine conjugate. Yet, these well-known phytohormones are only two among the numerous compounds that compose the jasmonate family. Here, we describe a method based on ultrahigh-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry that can potentially profile the full range of known free and esterified jasmonates in a non-targeted manner. The developed approach is illustrated by the analysis of Arabidopsis thaliana leaves after mechanical wounding.
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Acknowledgments
This work was supported by Swiss National Science Foundation grants no. 205320_135190 and CRSII3_127187. G.G. and J.L.W. are also grateful to the National Center of Competence in Research (NCCR) Plant Survival and to the Swiss Plant Science Web (SPSW).
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Glauser, G., Wolfender, JL. (2013). A Non-targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding. In: Goossens, A., Pauwels, L. (eds) Jasmonate Signaling. Methods in Molecular Biology, vol 1011. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-414-2_10
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DOI: https://doi.org/10.1007/978-1-62703-414-2_10
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