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Determining the Activity of the Transporter Associated with Antigen Processing in the Compartments of the Secretory Pathway

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Antigen Processing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 960))

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Abstract

Peptide-receptive MHC class I molecules and the TAP (transporter associated with antigen processing) peptide transporter are known to leave the ER and cycle through the cis side of the Golgi apparatus. The amount, and the extent of the activity, of TAP in post-ER compartments is likely to vary between different cell types. Here we describe a convenient microscopic assay to determine it.

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Acknowledgements

We acknowledge help by Ute Claus and Lana Ghuneim, and advice from Robert Tampé on the use of the ICP47 inhibitor (as well as a donatin of some ICP47). The work was funded by the DFG (German Research Council) and the DAAD (German Academic Exchange Service).

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Authors and Affiliations

  1. Molecular Life Science, Jacobs University Bremen, Bremen, Germany

    Esther Ghanem & Sebastian Springer

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  1. Esther Ghanem
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  2. Sebastian Springer
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Correspondence to Sebastian Springer .

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Editors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter van Endert

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Ghanem, E., Springer, S. (2013). Determining the Activity of the Transporter Associated with Antigen Processing in the Compartments of the Secretory Pathway. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_11

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  • DOI: https://doi.org/10.1007/978-1-62703-218-6_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-217-9

  • Online ISBN: 978-1-62703-218-6

  • eBook Packages: Springer Protocols

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