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Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging

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Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 906))

Abstract

In recent years, the near-infrared fluorescence (NIRF) labeled iron nanoparticles were synthesized and applied to labeling human cells for monitoring the engraftment process, imaging tumors, testing intracellular molecular environment surrounding the nanoparticles, and tracing biodistribution of nanoparticles in vivo. These studies demonstrated that the NIRF-labeled iron nanoparticles provided an excellent method not only for cell labeling but also for in vivo monitoring and tracing of iron nanoparticles due to the excellent in vivo imaging performance of the NIR fluorophores. However, the availability of commercial iron nanoparticles labeled with suitable NIRF dyes is limited. Optimal wavelength for in vivo imaging is centered at 800 nm, where tissue autofluorescence is minimal. Here we describe the manufacture of 12-nm 3-dimercaptosuccinic acid-coated Fe3O4 magnetic nanoparticles, their labeling with a new near-infrared fluorophore, IRDye800CW (excitation/emission: 778/806 nm), and their applications for cell labeling and in vivo imaging.

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Acknowledgments

This study was partially funded by the National Important Science Research Program of China (2006CB933205; 2011CB933503), and US-China international S & T cooperation project (2009DFA31990).

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Authors and Affiliations

  1. Experimental Center of Biotechnology and Biomaterials, BME, Southeast University, Nanjing, China

    Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen & Ning Gu

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  1. Jinke Wang
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  2. Yingxun Liu
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  3. Yong Hou
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  4. Zhongpin Chen
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  5. Ning Gu
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Corresponding author

Correspondence to Jinke Wang .

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Editors and Affiliations

  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

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Wang, J., Liu, Y., Hou, Y., Chen, Z., Gu, N. (2012). Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_17

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  • DOI: https://doi.org/10.1007/978-1-61779-953-2_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-952-5

  • Online ISBN: 978-1-61779-953-2

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