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Projection Printing of 3-Dimensional Tissue Scaffolds

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Computer-Aided Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 868))

Abstract

Our ability to create precise, predesigned, spatially patterned biochemical and physical microenvironments inside polymer scaffolds could provide a powerful tool in studying progenitor cell behavior and differentiation under biomimetic, three-dimensional (3D) culture conditions. The development of freeform fabrication technology has become a promising tool for the manufacturing of biological scaffolds for tissue regeneration and stem cell engineering. Freeform fabrication is a very promising technology due to the efficient and simple process for creating bona fide 3D microstructures, such as closed channels and cavities. It is also capable of encapsulating biomolecules and even living cells.

This chapter describes direct projection printing of 3D tissue engineering scaffolds by using a digital micromirror-array device (DMD) in a layer-by-layer process. This simple and fast microstereolithography system consists of an ultraviolet (UV) light source, a digital micromirror masking device, imaging optics, and controlling devices. Images of UV light are projected onto the photocurable resin by creating the “dynamic photomask” design with graphic software. Multilayered scaffolds are microfabricated through a photopolymerization process.

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Acknowledgment

This work was supported by the National Institutes of Health (R01EB012597).

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Authors and Affiliations

  1. Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX, USA

    Yi Lu

  2. Pearlie D. Henderson Centennial Endowed Faculty Fellow, Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX, USA

    Shaochen Chen

  3. Department of NanoEngineering, Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA

    Shaochen Chen

Authors
  1. Yi Lu
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  2. Shaochen Chen
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Corresponding author

Correspondence to Shaochen Chen .

Editor information

Editors and Affiliations

  1. , Department of Neurosurgery, Baylor College of Medicine, Holcombe Blvd. 2002, Houston, 77030, Texas, USA

    Michael A.K. Liebschner

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Lu, Y., Chen, S. (2012). Projection Printing of 3-Dimensional Tissue Scaffolds. In: Liebschner, M. (eds) Computer-Aided Tissue Engineering. Methods in Molecular Biology, vol 868. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-764-4_17

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  • DOI: https://doi.org/10.1007/978-1-61779-764-4_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-763-7

  • Online ISBN: 978-1-61779-764-4

  • eBook Packages: Springer Protocols

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