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MicroRNA Profiling Using µParaflo Microfluidic Array Technology

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Book cover Next-Generation MicroRNA Expression Profiling Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 822))

Abstract

The diverse functions of microRNA (miRNA) molecules have drawn broad and intensive interest in various biological fields, biomedical applications, and technology development. Which are endogeneous cellular short RNA molecules found in the cytoplasm as well as in various serum fluids. miRNAs are transcriptional and translational regulatory molecules active in cell division, growth, and apoptosis (1). Dysregulated expression of miRNAs has been implicated in various disease states and has been tested as biomarker candidates (2–4). miRNAs are endogeneous cellular short RNA molecules found in the cytoplasm as well as in various serum fluids. miRNAs are transcriptional and translational regulatory molecules active in cell division, growth, and apoptosis (Bartel, Cell 116:281–97, 2004). Dysregulated expression of miRNAs has been implicated in various disease states and has been tested as biomarker candidates (He et al., Nature 435:828–833, 2005; Lu et al., Nature 435:834–838, 2005; O’Donnell, et al., Nature 435:839–843, 2005). In this chapter, we describe the methods using μParaflo® microfluidic oligonucleotide microarray technology for applications in miRNA profiling. One unique feature of this technology is the flexibility that provides users with the freedom to select sequence content either for focused studies wherein only the most relevant sequences are included or for discovery studies wherein the most updated sequence content such as those newly derived from deep sequencing. This chapter provides detailed information from experimental design to sample preparation, as well as data analysis for a miRNA array experiment.

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Acknowledgments

This research is supported by grants from NIH (R44GM076941, R33CA126209, and R41DA029169).

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Authors and Affiliations

  1. LC Sciences, LLC, Houston, TX, USA

    Xiaochuan Zhou, Qi Zhu & Christoph Eicken

  2. Atactic Technologies Inc., Houston, TX, USA

    Nijing Sheng & Xiaolin Zhang

  3. Department of Biology and Biochemistry, University of Houston, Houston, TX, USA

    Litao Yang & Xiaolian Gao

Authors
  1. Xiaochuan Zhou
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  2. Qi Zhu
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  3. Christoph Eicken
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  4. Nijing Sheng
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  5. Xiaolin Zhang
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  6. Litao Yang
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  7. Xiaolian Gao
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Correspondence to Xiaolian Gao .

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  1. Illumina, Inc., Towne Centre Drive 9885, San Diego, 92121, California, USA

    Jian-Bing Fan

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Zhou, X. et al. (2012). MicroRNA Profiling Using µParaflo Microfluidic Array Technology. In: Fan, JB. (eds) Next-Generation MicroRNA Expression Profiling Technology. Methods in Molecular Biology, vol 822. Humana Press. https://doi.org/10.1007/978-1-61779-427-8_11

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  • DOI: https://doi.org/10.1007/978-1-61779-427-8_11

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