Abstract
Compared to other nanomaterials as supports for enzyme immobilization, nanofibers provide a promising configuration in balancing the key factors governing the catalytic performance of the immobilized enzymes including surface area-to-volume ratio, mass transfer resistance, effective loading, and the easiness to recycle. Synthetic and natural polymers can be fabricated into nanofibers via a physical process called electrospinning. The process requires only simple apparatus to operate, yet has proved to be very flexible in the selection of feedstock materials and also effective to control and manipulate the properties of the resulting nanofibers such as size and surface morphology, which are typically important parameters for enzyme immobilization supports. This chapter describes a protocol for the preparation of nanofibrous enzyme, involving the synthesis and end-group functionalization of polystyrene, production of electrospun nanofibers, and surface immobilization of enzyme via covalent attachment.
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References
Rozzell, J. D. (1999) Commercial scale biocatalysis: Myths and realities. Bioorg. Med. Chem. 7(10), 2253–2261.
Wandrey, C., Liese, A., and Kihumbu, D. (2000) Industrial biocatalysis: Past, present, and future. Org. Process Res. Dev. 4(4), 286–290.
Schmid, A., et al. (2001) Industrial biocatalysis today and tomorrow. Nature 409(6817), 11.
Kennedy, J. F., Melo, E. H. M., and Jumel, K. (1990) Immobilized enzymes and cells. Chem. Eng. Prog. 86(7), 81–89.
Tischer, W., and Wedekind, F. (1999) Immobilized enzymes: Methods and applications. Top. Curr. Chem. 200(Biocatalysis: From Discovery to Application), 95–126.
Kim, J., Grate, J. W., and Wang, P. (2009) Nanobiocatalysis and its potential applications. Trends Biotechnol. 26(11), 8.
Kim, J., Grate, J. W., and Wang, P. (2006) Nanostructures for enzyme stabilization. Chem. Eng. Sci. 61(3), 1017–1026.
Wang, P. (2006) Nanoscale biocatalyst systems. Curr. Opin. Biotechnol. 17(6), 574–579.
Wang, P. (2009) Multi-scale features in recent development of enzymic biocatalyst systems. Appl. Biochem. Biotechnol. 152(2), 343–352.
Wang, Z.-G., et al. (2009) Enzyme immobilization on electrospun polymer nanofibers: An overview. J. Mol. Catal. B: Enzym. 56(4), 7.
Xie, J., Li, X., and Xia, Y. (2008) Putting electrospun nanofibers to work for biomedical research. Macromol. Rapid Commun. 29(22), 18.
Li, D., and Xia, Y. N. (2004) Electrospinning of nanofibers: Reinventing the wheel? Adv. Mater. 16(14), 1151–1170.
Reneker, D. H., and Chun, I. (1996) Nanometer diameter fibers of polymer, produced by electrospinning. Nanotechnology 7(3), 216–223.
Kim, B. C., et al. (2008) Enzyme-nanofiber composites for biocatalysis applications. ACS Symp. Ser. 986(Biomolecular Catalysis), 9.
Wang, Y., and Hsieh, Y.-L. (2004) Enzyme immobilization to ultra-fine cellulose fibers via amphiphilic polyethylene glycol spacers. J. Polym. Sci., Part A: Polym. Chem. 42(17), 4289–4299.
Byoung Chan, K., et al. (2005) Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres. Nanotechnology 16(7), S382.
Jia, H., et al. (2002) Enzyme-carrying polymeric nanofibers prepared via electrospinning for use as unique biocatalysts. Biotechnol. Prog. 18(5), 1027–1032.
Zacchigna, M., et al. (1998) Properties of methoxy(polyethylene glycol)-lipase from Candida rugosa in organic solvents. Farmaco 53(12), 6.
Gabel, D. (1974) Active site titration of immobilized chymotrypsin with a fluorogenic reagent. FEBS Lett. 49(2), 2.
Acknowledgments
The protocol was originally developed at Prof. Ping Wang’s lab in the Department of Chemical Engineering at the University of Akron, with financial support from National Science Foundation NER program (Award #0103232).
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Jia, H. (2011). Enzyme-Carrying Electrospun Nanofibers. In: Wang, P. (eds) Nanoscale Biocatalysis. Methods in Molecular Biology, vol 743. Humana Press. https://doi.org/10.1007/978-1-61779-132-1_16
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DOI: https://doi.org/10.1007/978-1-61779-132-1_16
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