Abstract
DNA, protein and cell microarrays are increasingly used in a multitude of bioassays. All of these arrays require substrates that are suitable for the immobilisation and display of arrayed probe molecules whilst at the same time resisting non-specific interactions of biomolecules with the substrate in areas between printed spots. To meet these conflicting requirements, three different approaches have been developed, all of which were based on low-fouling, high-density poly(ethylene glycol) (PEG) background coatings. In the first approach, the coating was based on allylamine plasma polymerisation (ALAPP) and the subsequent high-density grafting of PEG, followed by the generation of a surface chemical pattern using laser ablation. In the second approach, a photoreactive polymer was printed on the same ALAPP–PEG background. The third approach was based on ALAPP deposition followed by the formation of a multifunctional layer by spin coating a PEG-based polymer that also displayed epoxy groups. The successful demonstration of DNA, protein and cell microarrays has been achieved on each of these coatings.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Meloni, R., Khalfallah, O., Biguet, N. F. (2004) DNA microarrays and pharmacogenomics. Pharmacol Res 49, 303–308.
Forster, T., Roy, D., Ghazal, P. (2003) Experiments using microarray technology: limitations and standard operating procedures. J Endocrinol 178, 195–204.
Kumar, A., Goel, G., Fehrenbach, E., Puniya, A. K., Singh, K. (2005) Microarrays: the technology, analysis and application. Eng Life Sci 5, 215–222.
Hook, A. L., Anderson, D. G., Langer, R., Williams, P., Davies, M. C., Alexander, M. R. (2010) High throughput methods applied in biomaterial development and discovery. Biomaterials 31, 187–198.
Hook, A. L., Voelcker, N., Thissen, H. (2009) Patterned and switchable surfaces for biomolecular manipulation. Acta Biomaterialia 5, 2350–2370.
Yasuda, H. (1985) Plasma Polymerization. Academic Press, Orlando, FL.
Cole, M. A., Voelcker, N. H., Thissen, H. (2007) Electro-induced protein deposition on low-fouling surfaces. Smart Mater Struct 16, 2222–2228.
Hook, A. L., Thissen, H., Quinton, J., Voelcker, N. H. (2008) Comparison of the binding mode of plasmid DNA to allylamine plasma polymer and poly(ethylene glycol) surfaces. Surf Sci 602, 1883–1891.
Thissen, H., Johnson, G., Hartley, P. G., Kingshott, P., Griesser, H. J. (2006) Two-dimensional patterning of thin coatings for the control of tissue outgrowth. Biomaterials 27, 35–43.
Hamerli, P., Weigel, T., Groth, T., Paul, D. (2003) Surface properties of and cell adhesion onto allylamine-plasma-coated polyethyleneterephthalate membranes. Biomaterials 24, 3989–3999.
Zhang, Z., Chen, Q., Knoll, W., Foerch, R., Holcomb, R., Roitman, D. (2003) Plasma polymer film structure and DNA probe immobilization. Macromolecules 36, 7689–7694.
Kingshott, P., Thissen, H., Griesser, H. J. (2002) Effects of cloud-point grafting, chain length, and density of PEG layers on competitive adsorption of ocular proteins. Biomaterials 23, 2043–2056.
Thissen, H., Hayes, J. P., Kingshott, P., Johnson, G., Harvey, E. C., Griesser, H. J. (2002) Nanometer thickness laser ablation for spatial control of cell attachment. Smart Mater Struct 11, 792–799.
Hook, A. L., Creasey, R., Hayes, J. P., Thissen, H., Voelcker, N. H. (2009) Laser based patterning of transfected cell microarrays. Biofabrication 1, 045003–045012.
Hook, A. L., Thissen, H., Hayes, J. P., Voelcker, N. H. (2005) Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications. Biosens Bioelectron 21, 2137–2145.
Hook, A. L., Thissen, H., Voelcker, N. H. (2009) Advanced substrate fabrication for cell microarrays. Biomacromolecules 10, 573–579.
Kurkuri, M. D., Driever, C., Johnson, G., McFarland, G., Thissen, H., Voelcker, N. H. (2009) Multifunctional polymer coatings for cell microarray applications. Biomacromolecules 10, 1163–1172.
Szili, E., Thissen, H., Hayes, J. P., Voelcker, N. (2004) A biochip platform for cell transfection assays. Biosens Bioelectron 19, 1395–1400.
Maurdev, G., Gee, M. L., Meagher, L. (2002) Controlling the adsorbed conformation and desorption of polyelectrolyte with added surfactant via the adsorption mechanism: a direct force measurement study. Langmuir 18, 9401–9408.
Griesser, H. J. (1989) Small-scale reactor for plasma processing of moving substrate web. Vacuum 39, 485–488.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Hook, A.L., Voelcker, N.H., Thissen, H. (2011). Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays. In: Palmer, E. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 706. Humana Press. https://doi.org/10.1007/978-1-61737-970-3_13
Download citation
DOI: https://doi.org/10.1007/978-1-61737-970-3_13
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61737-969-7
Online ISBN: 978-1-61737-970-3
eBook Packages: Springer Protocols