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Fluoroimmunoassays Using the NRL Array Biosensor

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Biosensors and Biodetection

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 503))

Array-based biosensor technology offers the user the ability to detect and quantify multiple targets in multiple samples simultaneously (Analytical Sciences 23:5–10, 2007). The NRL Array Biosensor has been developed with the aim of creating a system for sensitive, rapid, on-site screening for multiple targets of interest. This system is fluorescence-based, using evanescent illumination of a waveguide, and has demonstrated the use of both sandwich and competitive immunoassays for the detection of both high and low molecular weight targets, respectively. The current portable, automated system has demonstrated detection of a wide variety of analytes ranging from simple chemical compounds to entire bacterial cells, with applications in food safety, disease diagnosis, homeland security and environmental monitoring.

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Acknowledgments

The views, opinions, and/or findings described in this chapter are those of the authors and should not be construed as official Department of the Navy positions, policies, or decisions.

Author information

Authors and Affiliations

  1. Center for Bio/Molecular Science & Engineering, US Naval Research Laboratory, Washington, DC, USA

    Joel P. Golden & Kim E. Sapsford

Authors
  1. Joel P. Golden
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  2. Kim E. Sapsford
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Editor information

Editors and Affiliations

  1. FDA Center for Devices and Radiological Health, Silver Spring, MD, USA

    Avraham Rasooly

  2. National Cancer Institute, Bethesda, MD, USA

    Avraham Rasooly

  3. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

    Keith E. Herold

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC

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Golden, J.P., Sapsford, K.E. (2009). Fluoroimmunoassays Using the NRL Array Biosensor. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_15

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  • DOI: https://doi.org/10.1007/978-1-60327-567-5_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-566-8

  • Online ISBN: 978-1-60327-567-5

  • eBook Packages: Springer Protocols

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