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Microchip-Based Assay Systems pp 53–64Cite as

Microchip-Based Enumeration of Human White Blood Cells

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 385))

Abstract

The advent of flow cytometry has considerably changed the ways in which medical testing is conducted. However, the cost of flow cytometers, their large size, and their maintenance needs make them scarce in resource-poor settings and available almost only in clinical pathology laboratories in developed countries. Because cell enumeration is a basic and crucial support of diagnosis, prognosis, and treatment, an alternative cell-counting method that would potentially be cost-effective, portable, and suitable for use in resource-poor settings is warranted. We describe here a protocol for conducting cell-counting experiments in a simple microfluidic structure. This protocol describes how to build a simple microfluidic cell and perform a total white blood cell (WBC) count through capture and immunolabeling of the WBCs with an anti-CD45 antibody.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX

    Pierre N. Floriano, Shelley Acosta, Nicolaos Christodoulides, Shannon Weigum & John T. McDevitt

Authors
  1. Pierre N. Floriano
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  2. Shelley Acosta
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  3. Nicolaos Christodoulides
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  4. Shannon Weigum
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  5. John T. McDevitt
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Editor information

Editors and Affiliations

  1. University of Texas at Austin, Austin, TX

    Pierre N. Floriano

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© 2007 Humana Press Inc., Totowa, NJ

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Floriano, P.N., Acosta, S., Christodoulides, N., Weigum, S., McDevitt, J.T. (2007). Microchip-Based Enumeration of Human White Blood Cells. In: Floriano, P.N. (eds) Microchip-Based Assay Systems. Methods in Molecular Biology™, vol 385. Humana Press. https://doi.org/10.1007/978-1-59745-426-1_5

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  • DOI: https://doi.org/10.1007/978-1-59745-426-1_5

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-588-0

  • Online ISBN: 978-1-59745-426-1

  • eBook Packages: Springer Protocols

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