Abstract
Recent advances in antibody microarray technology have facilitated the development of multiplexed diagnostic platforms. Highly parallel antigen expression data obtained from these arrays allow disease states to be characterized using protein patterns rather than individual protein markers. The development of an antibody microarray platform of general applicability requires careful consideration of the array content. The human cluster of differentiation (CD) antigens constitute a promising candidate set, being united by their common expression at the leukocyte cell surface and the fact that the majority perform critical functions in the human immune response. The diagnostic potential of a microarray, containing 82 cluster of differentiation monoclonal antibodies (DotScan microarrays) has been demonstrated for a variety of infectious and neoplastic disease states, including HIV, many acute and chronic leukemias, and colorectal cancer. It is likely that these microarrays will have more general utility that extends to other pathological categories, including autoimmune, metabolic, and degenerative diseases.
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References
1. Schoniger-Hekele M, Muller C (2006) The combined elevation of tumour markers CA 19-9 and CA 125 in liver disease patients is highly specific for severe liver fibrosis. Dig Dis Sci 51:338–345
2. Louhimo J, Finne P, Alfthan H, Stenman UH, Haglund C (2002) Combination of HCGbeta, CA 19-9 and CEA with logistic regression improves accuracy in gastrointestinal malignancies. Anticancer Res 22:1759–1764
3. Woolfson A, Ellmark P, Chrisp JS, Scott MA, Christopherson RI (2006) The application of CD antigen proteomics to pharmacogenomics. Pharmacogenomics 7:759–771
4. Borrebaeck CA (2006) Antibody microarray-based oncoproteomics. Expert Opin Biol Ther 6:833–838
5. Lal SP, Christopherson RI, dos Remedios CG (2002) Antibody arrays: An embryonic but rapidly growing technology. Drug Discov Today 7:S143–S149
Haab BB, Dunham MJ, Brown PO (2001) Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. Genome Biol 2 RESEARCH0004
7. MacBeath G, Schreiber SL (2000) Printing proteins as microarrays for high-throughput function determination. Science 289:1760–1763
8. Knezevic V, Leethanaku, C, Bichsel VE, Worth JM, Prabhu VV, Gutkind JS, Liotta LA, Munson PJ, Petricoin EF III, Krizman DB (2001) Proteomic profiling of the cancer microenvironment by antibody arrays. Proteomics 1:1271–1278
9. Belov L, de la Vega O, dos Remedios CG, Mulligan SP, Christopherson RI (2001) Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. Cancer Res 61:4483–4489
10. Woolfson A, Stebbing J, Tom BD, Stoner KJ, Gilks WR, Kreil DP, Mulligan SP, Belov L, Chrisp JS, Errington W, Wildfire A, Erber WN, Bower M, Gazzard B, Christopherson RI, Scott MA (2005) Conservation of unique cell-surface CD antigen mosaics in HIV-1-infected individuals. Blood 106:1003–1007
11. Ellmark P, Ingvarsson J, Carlsson A, Lundin SB, Wingren C, Borrebaeck CA (2006) Identification of protein expression signatures associated with H. pylori infection and gastric adenocarcinoma using recombinant antibody microarrays. Mol Cell Proteomics 5:1638–46
12. Miller JC, Zhou H, Kwekel J, Cavallo R, Burke J, Butler EB, Teh BS, Haab BB (2003) Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers. Proteomics 3:56–63
13. Orchekowski R, Hamelinck D, Li L, Gliwa E, vanBrocklin M, Marrero JA, Vande Woude GF, Feng Z, Brand R, Haab BB (2005) Antibody microarray profiling reveals individual and combined serum proteins associated with pancreatic cancer. Cancer Res 65:11193–11202
14. Ellmark P, Belov L, Huang P, Lee CS, Solomon MJ, Morgan DK, Christopherson RI (2006) Multiplex detection of surface molecules on colorectal cancers. Proteomics 6:1791–1802
15. Belov L, Huang P, Barber N, Mulligan SP, Christopherson RI (2003) Identification of repertoires of surface antigens on leukemias using an antibody microarray. Proteomics 3:2147–2154
16. Belov L, Mulligan SP, Barber N, Woolfson A, Scott M, Stoner K, Chrisp JS, Sewell WA, Bradstock KF, Bendall L, Pascovici DS, Thomas M, Erber W, Young GAR, Wiley JS, Juneja S, Wierda WG, Green AR, Keating MJ and Christopherson RI (2006) Classification of human leukemias and lymphomas using extensive immunophenotypes obtained by cell capture on an antibody microarray. Brit J Haem 135:184–197
17. Carter PJ (2006) Potent antibody therapeutics by design. Nat Rev Immunol 6:343–357
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Ellmark, P., Woolfson, A., Belov, L., Christopherson, R.I. (2008). The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease. In: Starkey, M., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 439. Humana Press. https://doi.org/10.1007/978-1-59745-188-8_14
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DOI: https://doi.org/10.1007/978-1-59745-188-8_14
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