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Thioredoxin-1 PEGylation as an In Vitro Method for Drug Target Identification

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Book cover Redox-Mediated Signal Transduction

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1990))

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Abstract

Redox signaling in the cell, which is essential for cell physiology, involves proteins with free sulfhydryl groups (−SH). Among them, the thioredoxin system plays the most significant role. Many conditions associated with cell malignancies feature the higher expression of thioredoxin, making it an attractive target for new therapeutic drug development. Here we present a simple in vitro model of testing the interaction between thioredoxin and the putative drug. This method is relatively inexpensive and gives the Investigator a first screen of the drug properties, which can be essential for further experimental approaches.

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Author information

Authors and Affiliations

  1. College of Liberal Arts and Sciences, Alfred University, Alfred, NY, USA

    Jolanta Skalska

  2. Captor Therapeutics SA, Dunska, Wroclaw, Poland

    Jolanta Skalska

Authors
  1. Jolanta Skalska
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Correspondence to Jolanta Skalska .

Editor information

Editors and Affiliations

  1. Department of Applied Sciences, University of the West of England, Bristol, UK

    John T. Hancock

  2. Department of Applied Sciences, University of the West of England, Bristol, UK

    Myra E. Conway

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Skalska, J. (2019). Thioredoxin-1 PEGylation as an In Vitro Method for Drug Target Identification. In: Hancock, J., Conway, M. (eds) Redox-Mediated Signal Transduction. Methods in Molecular Biology, vol 1990. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9463-2_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9461-8

  • Online ISBN: 978-1-4939-9463-2

  • eBook Packages: Springer Protocols

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