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Development of the Histoculture Drug Response Assay (HDRA)

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Book cover 3D Sponge-Matrix Histoculture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1760))

Abstract

The histoculture drug response assay (HDRA) was developed using Gelfoam® histoculture of all tumor types. Twenty tumor classes, including all the major ones, have been histocultured on Gelfoam® and tested for drug response. Quantitative and qualitative results show increasing cell kill with rising cytotoxic drug concentration, differential drug sensitivities of multiple cell types within individual cultured tumors, differential sensitivities to a single drug of a series of tumors of the same histopathological classification, differential sensitivities of individual tumors to a series of drugs, and sensitivity patterns of various tumor types similar to the sensitivities found in vivo. Therefore, the results indicated that precise therapeutic data can be obtained from tumor specimens growing in Gelfoam® histoculture in vitro for the individual cancer patient as well as for rational and relevant screening for novel agents active against human solid tumors.

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Author information

Authors and Affiliations

  1. AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  2. Department of Surgery, UCSD, San Diego, CA, USA

    Robert M. Hoffman

  3. Multiple Myeloma and Amyloidosis Program, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA

    Robert A. Vescio

Authors
  1. Robert M. Hoffman
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  2. Robert A. Vescio
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Correspondence to Robert M. Hoffman .

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Editors and Affiliations

  1. AntiCancer, Inc., San Diego, California, USA

    Robert M. Hoffman

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Hoffman, R.M., Vescio, R.A. (2018). Development of the Histoculture Drug Response Assay (HDRA). In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7745-1_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7743-7

  • Online ISBN: 978-1-4939-7745-1

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