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3D Sponge-Matrix Histoculture pp 11–17Cite as

3D Sponge-Matrix Histoculture: An Overview

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1760))

Abstract

Three-dimensional cell culture and tissue culture (histoculture) is much more in vivo-like than 2D culture on plastic. Three-dimensional culture allows investigation of crucial events in tumor biology such as drug response, proliferation and cell cycle progression, cancer cell migration, invasion, metastasis, immune response, and antigen expression that mimic in vivo conditions. Three-dimensional sponge-matrix histoculture maintains the in vivo phenotype, including the formation of differentiated structures of normal and malignant tissues, perhaps due to cells maintaining their natural shape in a sponge-gel matrix such as Gelfoam®. Sponge-matrix histoculture can also support normal tissues and their function including antibody-producing lymphoid tissue that allows efficient HIV infection, hair-growing skin, excised hair follicles that grow hair, pluripotent stem cells that form nerves, and much more.

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Authors and Affiliations

  1. AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  2. Department of Surgery, UCSD, San Diego, CA, USA

    Robert M. Hoffman

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  1. Robert M. Hoffman
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Correspondence to Robert M. Hoffman .

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  1. AntiCancer, Inc., San Diego, California, USA

    Robert M. Hoffman

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Hoffman, R.M. (2018). 3D Sponge-Matrix Histoculture: An Overview. In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7745-1_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7743-7

  • Online ISBN: 978-1-4939-7745-1

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