Abstract
Autophagy is an intercellular degradation/recycling system by which cytoplasmic components are sequestered in autophagic vesicles (autophagosomes) and delivered to the vacuole for breakdown. During the last decade, plant studies have revealed that autophagy is employed as a major route to recycle nutrients needed for plant growth and development, and to combat with a wide range of biotic and abiotic stresses. Another important outcome of these studies was the development and optimization of methods and techniques for monitoring autophagy activity in plants. In this chapter, methods frequently used in plant autophagy study, from physiological to biochemical and microscopical analyses, are discussed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (Project 31370298), Program for New Century Excellent Talents in University (Project NCET-13-0614), and Sun Yat-sen University (funds to S.X.).
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Chen, L., Li, F., Xiao, S. (2017). Analysis of Plant Autophagy. In: Jiang, L. (eds) Plant Protein Secretion. Methods in Molecular Biology, vol 1662. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7262-3_24
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DOI: https://doi.org/10.1007/978-1-4939-7262-3_24
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