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Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture

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Heterologous Protein Production in CHO Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1603))

Abstract

Chinese hamster ovary (CHO) cells have become the primary expression system for the production of complex recombinant proteins due to their long-term success in industrial scale production and generating appropriate protein N-glycans similar to that of humans. Control and optimization of protein N-glycosylation is crucial, as the structure of N-glycans can largely influence both biological and physicochemical properties of recombinant proteins. Protein N-glycosylation in CHO cell culture can be controlled and tuned by engineering medium, feed, culture process, as well as genetic elements of the cell. In this chapter, we will focus on how to carry out experiments for N-glycosylation modulation through medium and feed optimization. The workflow and typical methods involved in the experiment process will be presented.

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Authors and Affiliations

  1. Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark

    Yuzhou Fan & Mikael Rørdam Andersen

  2. The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark

    Yuzhou Fan & Helene Faustrup Kildegaard

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  1. Yuzhou Fan
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  2. Helene Faustrup Kildegaard
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  3. Mikael Rørdam Andersen
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Correspondence to Yuzhou Fan or Mikael Rørdam Andersen .

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  1. National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland

    Paula Meleady

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Fan, Y., Kildegaard, H.F., Andersen, M.R. (2017). Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture. In: Meleady, P. (eds) Heterologous Protein Production in CHO Cells. Methods in Molecular Biology, vol 1603. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6972-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6972-2_14

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