Skip to main content
Book cover

Xylem pp 21–26Cite as

Strigolactone-mediated Stimulation of Secondary Xylem Proliferation in Stems

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1544))

Abstract

Secondary xylem (wood) accounts for a large proportion of the terrestrial biomass. Understanding how secondary xylem develops and proliferates is a challenge to enhance our capacities for biomass production. Recent reports revealed that the plant hormone strigolactone is key for the development of secondary xylem. Here, I describe a protocol for strigolactone-mediated stimulation of secondary xylem proliferation in stems. The protocol has been tested in Arabidopsis and Eucalyptus and can be adjusted to other species.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Agusti J, Herold S, Schwarz M, Sanchez P, Ljung K, Dun EA, Brewer PB, Beveridge CA, Sieberer T, Sehr EM, Greb T (2011) Strigolactone signaling is required for auxin-dependent stimulation of secondary growth in plants. Proc Natl Acad Sci U S A 108(50):20242–20247

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Gomez-Roldan V, Fermas S, Brewer PB, Puech-Pages V, Dun EA, Pillot JP, Letisse F, Matusova R, Danoun S, Portais J-C, Bowmeester H, Becard G, Beveridge CA, Rameau C, Rochange SF (2008) Strigolactone inhibition of shoot branching. Nature 455:189–195

    Article  CAS  PubMed  Google Scholar 

  3. Agusti J, Greb T (2013) Going with the wind—adaptive dynamics of secondary meristems in plants. Mech Dev 130(1):34–44

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Agusti J, Lichtenberger R, Schwarz M, Nehlin L, Greb T (2011) Characterization of transcriptome remodelling during cambium formation identifies MOL1 and RUL1 as opposing regulators of secondary growth. PLoS Genet 7(2):e1001312. doi:10.1371/journal.pgen.1001312

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Sehr EM, Agusti J, Lehner R, Farmer EE, Schwarz M, Greb T (2010) Analysis of secondary growth in the Arabidopsis shoot reveals a positive role of jasmonate signaling in cambium formation. Plant J 63:811–822

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Javier Agusti .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Agusti, J. (2017). Strigolactone-mediated Stimulation of Secondary Xylem Proliferation in Stems. In: de Lucas, M., Etchhells, J. (eds) Xylem. Methods in Molecular Biology, vol 1544. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6722-3_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6722-3_2

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6720-9

  • Online ISBN: 978-1-4939-6722-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics