Skip to main content
SpringerLink
Log in

The Chorioallantoic Membrane of the Chick Embryo to Assess Tumor Formation and Metastasis

  • Protocol
  • First Online:
Book cover Tumor Angiogenesis Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1464))

Abstract

The chorioallantoic membrane (CAM) of the chick embryo is a suitable and convenient platform for the assessment of tumor formation and metastatic dissemination. Here, we describe tumor cell engraftment on the extraembryonic CAM and further monitoring of tumor growth and metastasis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Rous P, Murphy JB (1911) Tumor implantations in the developing embryo. Experiments with a transmissible sarcoma of the fowl. J Am Med Assoc 56:741–742

    Google Scholar 

  2. Ribatti D (2008) The chick embryo chorioallantoic membrane in the study of tumor angiogenesis. Rom J Morphol Embryol 49:131–135

    CAS  PubMed  Google Scholar 

  3. Meirowsky E, Freeman LW, Woodard JS (1954) The response of embryonic chick membrane to Bowen’s intraepithelial cancer. J Invest Dermatol 22:417–429

    Article  CAS  PubMed  Google Scholar 

  4. Herrmann A, Rice M, Levy R et al (2015) Cellular memory of hypoxia elicits neuroblastoma metastasis and enables invasion by non-aggressive neighbouring cells. Oncogenesis 4:e138

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Carter R, Mullassery D, See V et al (2012) Exploitation of chick embryo environments to reprogram MYCN-amplified neuroblastoma cells to a benign phenotype, lacking detectable MYCN expression. Oncogenesis 1:e24

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Liverpool, Biosciences Bldg., Liverpool, L69 7ZB, UK

    Anne Herrmann & Violaine Sée

  2. Department of Cellular and Molecular Physiology, University of Liverpool, Liverpool, L69 3BX, UK

    Anne Herrmann & Diana Moss

Authors
  1. Anne Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Diana Moss
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Violaine Sée
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Violaine Sée .

Editor information

Editors and Affiliations

  1. Neurosciences and Sensory Organs, University of Bari,School of Med Dept of Basic Med Sci, Bari, Italy

    Domenico Ribatti

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Herrmann, A., Moss, D., Sée, V. (2016). The Chorioallantoic Membrane of the Chick Embryo to Assess Tumor Formation and Metastasis. In: Ribatti, D. (eds) Tumor Angiogenesis Assays. Methods in Molecular Biology, vol 1464. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3999-2_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3999-2_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3997-8

  • Online ISBN: 978-1-4939-3999-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation