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Zebrafish pp 3–16Cite as

Chemical Screening in Zebrafish

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1451))

Abstract

Phenotypic small molecule screens in zebrafish have gained popularity as an unbiased approach to probe biological processes. In this chapter we outline basic methods for performing chemical screens with larval zebrafish including breeding large numbers of embryos, plating larval fish into multi-well dishes, and adding small molecules to these wells. We also highlight important considerations when designing and interpreting the results of a phenotypic screen and possible follow-up approaches, including popular methods used to identify the mechanism of action of a chemical compound.

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Author information

Authors and Affiliations

  1. Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA

    Colleen A. Brady, Andrew J. Rennekamp & Randall T. Peterson

  2. Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA

    Colleen A. Brady, Andrew J. Rennekamp & Randall T. Peterson

  3. Broad Institute, 7 Cambridge Center, Cambridge, MA, 02142, USA

    Colleen A. Brady, Andrew J. Rennekamp & Randall T. Peterson

Authors
  1. Colleen A. Brady
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  2. Andrew J. Rennekamp
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  3. Randall T. Peterson
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Corresponding author

Correspondence to Randall T. Peterson .

Editor information

Editors and Affiliations

  1. Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

    Koichi Kawakami

  2. Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom

    E. Elizabeth Patton

  3. Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal

    Michael Orger

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Brady, C.A., Rennekamp, A.J., Peterson, R.T. (2016). Chemical Screening in Zebrafish. In: Kawakami, K., Patton, E., Orger, M. (eds) Zebrafish. Methods in Molecular Biology, vol 1451. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3771-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3771-4_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3769-1

  • Online ISBN: 978-1-4939-3771-4

  • eBook Packages: Springer Protocols

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