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Clinical Applications of Mass Spectrometry in Drug Analysis pp 21–27Cite as

Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1383))

Abstract

This chapter describes a method to measure the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma samples using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The instrument is operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source in positive ionization mode. Samples are extracted with a 90:10 methanol/0.1 N hydrochloric acid solution containing stable isotope-labeled internal standards for each analyte. After centrifugation the supernatant is transferred to a mass spectrometry vial, injected onto the UPLC-ESI-MS/MS, and quantified using an eight-point calibration curve.

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References

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Author information

Authors and Affiliations

  1. Department of Pathology, University Hospitals Case Medical Center, 11100 Euclid Avenue, PTH 5077, Cleveland, OH, 44106, USA

    Jaime H. Noguez Ph.D.

  2. Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, USA

    James C. Ritchie

Authors
  1. Jaime H. Noguez Ph.D.
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  2. James C. Ritchie
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Corresponding author

Correspondence to Jaime H. Noguez Ph.D. .

Editor information

Editors and Affiliations

  1. Dept of Pathology & Lab Medicine, Children's Mercy Hospitals & Clinics, Kansas City, Missouri, USA

    Uttam Garg

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Noguez, J.H., Ritchie, J.C. (2016). Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS). In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 1383. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3252-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3252-8_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3251-1

  • Online ISBN: 978-1-4939-3252-8

  • eBook Packages: Springer Protocols

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