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The “Clickable” Method for Oligonucleotide Immobilization Onto Azide-Functionalized Microarrays

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Microarray Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1368))

Abstract

The DNA microarray technique was supposed to help identifying and analyzing the expression level of tens of thousands of genes in the whole genome. But there is a serious problem concerning fabrication of the microarrays by chemical synthesis, such as specific and efficient linking of probes to a solid support. Therefore, we reckon that applying “click” chemistry to covalently anchor oligonucleotides on chemically modified supports may help construct microarrays in applications such as gene identification. Silanization of the glass support with organofunctional silane makes it possible to link azide groups on glass surface and the nucleic acid probe that is equipped with a pentynyl group. This is followed by direct spotting of the nucleic acid on the azide-modified glass support in the presence of copper ions, and this is a frequently applied method of “click” chemistry.

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Acknowledgment

This research was supported by the European Regional Development Fund within the Innovative Economy Programme [Grant No. POIG.01.03.01-30-045].

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Author notes

    Authors and Affiliations

    1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland

      Tomasz Ratajczak & Marcin K. Chmielewski

    2. Centre for Genomic Regulation (CGR), Dr. Aiguader 88, 08003, Barcelona, Catalonia, Spain

      Barbara Uszczyńska

    3. Universitat Pompeu Fabra (UPF), Barcelona, Spain

      Barbara Uszczyńska

    4. Poznan Science and Technology Park, Adam Mickiewicz University Foundation, Rubiez 46, 61-612, Poznan, Poland

      Emilia Frydrych-Tomczak

    Authors
    1. Tomasz Ratajczak
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    2. Barbara Uszczyńska
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    3. Emilia Frydrych-Tomczak
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    4. Marcin K. Chmielewski
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    Corresponding author

    Correspondence to Marcin K. Chmielewski .

    Editor information

    Editors and Affiliations

    1. Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada

      Paul C.H. Li

    2. Dept. of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada

      Abootaleb Sedighi

    3. Department of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada

      Lin Wang

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    Ratajczak, T., Uszczyńska, B., Frydrych-Tomczak, E., Chmielewski, M.K. (2016). The “Clickable” Method for Oligonucleotide Immobilization Onto Azide-Functionalized Microarrays. In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_3

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    • DOI: https://doi.org/10.1007/978-1-4939-3136-1_3

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3135-4

    • Online ISBN: 978-1-4939-3136-1

    • eBook Packages: Springer Protocols

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