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Pulsed-Field Gel Electrophoresis for Disease Monitoring and Control

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Pulse Field Gel Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1301))

Abstract

Pulsed-field gel electrophoresis (PFGE) is the “gold standard” for molecular subtyping and has significant applications in disease monitoring and control programs. This chapter discusses the advantages of PFGE in light of developing technologies such as whole-genome sequencing.

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References

  1. Schwartz DC, Cantor CR (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37(1):67–75

    Article  CAS  PubMed  Google Scholar 

  2. Van der Ploeg LH, Schwartz DC, Cantor CR, Borst P (1984) Antigenic variation in Trypanosoma brucei analysed by electrophoretic separation of chromosome-sized DNA molecules. Cell 37(1):77–84

    Article  PubMed  Google Scholar 

  3. Powell NG, Threlfall EJ, Chart H, Rowe B (1994) Subdivision of Salmonella enteritidis PT 4 by pulsed-field gel electrophoresis: potential for epidemiological surveillance. FEMS Microbiol Lett 119(1–2):193–198

    Article  CAS  PubMed  Google Scholar 

  4. Thong KL, Cheong YM, Puthucheary S, Koh CL, Pang T (1994) Epidemiologic analysis of sporadic Salmonella typhi isolates and those from outbreaks by pulsed-field gel electrophoresis. J Clin Microbiol 32(5):1135–1141

    CAS  PubMed Central  PubMed  Google Scholar 

  5. Beall B, Cassiday PK, Sanden GN (1995) Analysis of Bordetella pertussis isolates from an epidemic by pulsed-field gel electrophoresis. J Clin Microbiol 33(12):3083–3086

    CAS  PubMed Central  PubMed  Google Scholar 

  6. Stewart PR, el-Adhami W, Inglis B, Franklin JC (1993) Analysis of an outbreak of variably methicillin-resistant Staphylococcus aureus with chromosomal RFLPs and mec region probes. J Med Microbiol 38(4):270–277

    Article  CAS  PubMed  Google Scholar 

  7. Hanifah YA, Hiramatsu K (1994) Pulsed-field gel electrophoresis of chromosomal DNA of methicillin-resistant Staphylococcus aureus associated with nosocomial infections. Malays J Pathol 16(2):151–156

    CAS  PubMed  Google Scholar 

  8. Hennessy TW, Hedberg CW, Slutsker L, White KE, Besser-Wiek JM, Moen ME, Feldman J, Coleman WW, Edmonson LM, MacDonald KL, Osterholm MT (1996) A national outbreak of Salmonella enteritidis infections from ice cream. N Engl J Med 334(20):1281–1286

    Article  CAS  PubMed  Google Scholar 

  9. Jacobson JA, Chester TJ, Fraser DW (1997) An epidemic of disease due to serogroup B Neisseria meningitidis in Alabama: report of an investigation and community-wide prophylaxis with a sulfonamide. J Infect Dis 136(1):104–108

    Article  Google Scholar 

  10. Green MJ, Cawley PF (1980) Antimicrobial susceptibility of Neisseria meningitidis isolated from 1975-1979. N Z Med J 92(672):380–382

    CAS  PubMed  Google Scholar 

  11. Bender JB, Hedberg CW, Besser JM, Boxrud DJ, MacDonald KL, Osterholm MT (1997) Surveillance by molecular subtype for Escherichia coli O157:H7 infections in Minnesota by molecular subtyping. N Engl J Med 337(6):388–394

    Article  CAS  PubMed  Google Scholar 

  12. Bender JB, Hedberg CW, Boxrud DJ, Besser JM, Wicklund JH, MacDonald KL, Osterholm MT (2001) Molecular subtype surveillance of Salmonella typhimurium, Minnesota, 1994-1998. NEJM 344:189–195

    Article  CAS  PubMed  Google Scholar 

  13. Swaminathan B, Barrett TJ, Hunter SB, Tauxe RV, CDC PulseNet Task Force (2001) PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis 7(3):382–389

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. McDougal LK, Steward CD, Killgore GE, Chaitram JM, McAllister SK, Tenover FC (2003) Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: establishing a national database. J Clin Microbiol 41(11):5113–5120

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE, Fussing V, Salmenlinna S, Vuopio-Varkila J, El Solh N, Cuny C, Witte W, Tassios PT, Legakis N, van Leeuwen W, van Belkum A, Vindel A, Laconcha I, Garaizar J, Haeggman S, Olsson-Liljequist B, Ransjo U, Coombes G, Cookson B (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41(4):1574–1585. doi:10.1128/JCM. 41.4.1574-1585.2003

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Rainbow J, Jewell B, Danila RN, Boxrud D, Beall B, Van Beneden C, Lynfield R (2008) Invasive group a streptococcal disease in nursing homes, Minnesota, 1995-2006. Emerg Infect Dis 14(5):772–777. doi:10.3201/eid1405.070407

    PubMed Central  PubMed  Google Scholar 

  17. Popovic T, Schmink S, Rosenstein NA, Ajello GW, Reeves MW, Plikaytis B, Hunter SB, Ribot EM, Boxrud D, Tondella ML, Kim C, Noble C, Mothershed E, Besser J, Perkins BA (2001) Evaluation of pulsed-field gel electrophoresis in epidemiological investigations of meningococcal disease outbreaks caused by Neisseria meningitidis serogroup C. J Clin Microbiol 39(1):75–85

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Allard MW, Luo Y, Strain E, Pettengill J, Timme R, Wang C, Li C, Keys CE, Zheng J, Stones R, Wilson MR, Musser SM, Brown EW (2013) On the evolutionary history, population genetics and diversity among isolates of Salmonella Enteritidis PFGE pattern JEGX01.0004. PLoS One 8(1):e55254

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33(9):2233–2239

    CAS  PubMed Central  PubMed  Google Scholar 

  20. Reingold AL (1998) Outbreak investigations—a perspective. Emerg Infect Dis 4:21–27

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Correspondence to John Besser .

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Besser, J. (2015). Pulsed-Field Gel Electrophoresis for Disease Monitoring and Control. In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2599-5_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2598-8

  • Online ISBN: 978-1-4939-2599-5

  • eBook Packages: Springer Protocols

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