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Transgene-Induced Gene Silencing in Plants

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Plant Gene Silencing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1287))

Abstract

RNAi is the most important reverse genetics tool to trigger transgenic gene silencing, which is now applied widely to investigate gene function and also practically applied to enhance resistance to biotic and abiotic stress. Recently, the most effective way to induce transgenic gene silencing is to introduce inverted repeat (IR) double-stranded RNA (dsRNA) or artificial microRNA (amiRNA) instead of a transgenic sense or antisense strand of genes. The stable transgenic plants can be acquired through Agrobacterium tumefaciens-mediated transformation of binary vectors containing an RNAi hairpin construct or amiRNA precursor backbone sequence. Here we primarily describe these two methods’ vector construction, plant transformation, and transgenic line verification.

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Acknowledgments

This work was supported by grants from the Natural Science Foundation of China (91219301 and 31123007) and the Ministry of Science and Technology of China (2011CB100703).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, 100101, China

    Yun Jin & Hui-Shan Guo

Authors
  1. Yun Jin
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  2. Hui-Shan Guo
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Corresponding author

Correspondence to Hui-Shan Guo .

Editor information

Editors and Affiliations

  1. Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA

    Kirankumar S. Mysore

  2. National Institute of Plant Genome Research, New Delhi, Delhi, India

    Muthappa Senthil-Kumar

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Jin, Y., Guo, HS. (2015). Transgene-Induced Gene Silencing in Plants. In: Mysore, K., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 1287. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2453-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-2453-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2452-3

  • Online ISBN: 978-1-4939-2453-0

  • eBook Packages: Springer Protocols

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