Abstract
Lipids are generally defined as fatty acids, alcohols, hydrocarbons, and compounds containing these substances which are soluble in organic solvents. The lipids most commonly found in bacteria are phospholipids, glycolipids, ornithine amide lipids, fatty acids, and lipopolysaccharides. Phospholipids generally constitute ~40% of the cytoplasmic membrane of bacteria and up to 25% of the outer membrane (mainly localized in the inner leaflet). A generalized structure for a Pseudomonas membrane is shown in Figure 1. It has been found that the predominant phospholipid in both the inner and outer membranes in most Pseudomonas species is phosphatidylethanolamine (Wilkinson, 1988). Ornithine amide lipids are localized in the outer membrane. Lipopolysaccharides are located in the outer leaflet of the outer membrane of gram-negative bacteria. Glycolipids are generally found as storage lipids located in intracellular inclusions but can also be found in the membranes of P. diminuta and P. vesicularis and gram-positive bacteria (Wilkinson, 1988). Carotenoids and hydrocarbons may be found in the cytoplasmic membrane.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anderson, A. J., and Dawes, E. A., 1990, Occurence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkoanates, Microbiol. Rev. 54: 450–472.
Bhakoo, M., and Herbert, R. A., 1989, Fatty acid and phospholipid composition of five psychrotrophic Pseudomonas species grown at different temperatures. Arch. Microbiol. 126: 51–5.
Bouzar, H., Jones, J. B., Stall, R. E., Hodge, N. C, Minsavage, G. V., Benedict, A. A., and Alverez, A. M., 1994, Physiological, chemical, serological, and pathogenic analysis of a worldwide collection of Xanthomonas campestris pv. vesicatoria strains, Phytopathology 84: 663–671.
Boulton, C. A., and Ratledge, C., 1987, Biosynthesis of lipid precursors to surfactant production, in: Biosurfactants and Biotechnology (N. Kosaric, W. L. Cairns, and N. C. C. Gray, eds.), M. Dekker, New York, pp. 47–87.
Brint, J. M., and Ohman, D., 1995, Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR and RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family, J. Bacteriol. 177: 7155–7163.
Burger, M. M., Glaser, L., and Burton, R. M., 1963, The enzymatic synthesis of a rhamnose-containing glycolipid by extracts of Pseudomonas aeruginosa, J. Biol. Chem. 238: 2595–2604.
de Andres, C., Espuny, M. J., Robert, M., Mercade, M. E., Manresa, A., and Guinea, J., 1991, Cellular lipid accumulation by Pseudomonas aeruginosa 44T1, Appl. Microbiol. Biotechnol. 35: 813–816.
Dees, S. B., Hollis, D. G., Weaver, R. E., and Moss, C. W., 1983, Cellular fatty acid composition of Pseudomonas marginata and closely related bacteria, J. Clin. Microbiol. 18: 1073–1078.
Denny, T. P., 1988, Phenotypic diversity in Pseudomonas syringae pv. tomato, J. Gen. Microbiol. 134: 1939–1948.
de Smet, M. J., Eggink, G., Witholt, B., Kingma, J., and Wynberg, H., 1983, Characterization of cellular inclusions formed by Pseudomonas oleovorans during growth on octaine, J. Bacteriol. 154: 870–878.
de Waard, P., van der Wal, H., Huijberts, G. N. M., and Eggink, G., 1993, Heteronuclear NMR analysis of unsaturated fatty acids in poly(3-hydroxyalkoanates): Study of betaoxidation in Pseudomonas putida. J. Biol. Chem. 268: 315–319.
Edwards, R. A., Dainty, R. H., and Hibbard, C. M., 1987, Volatile compounds produced by meat pseudomonads and related reference strains during growth on beef stored in air at chill temperatures, J. Appl. Bacteriol. 62: 403–412.
Finnerty, W. R., 1994, Biosurfactants in environmental biotechnology. Curr. Op. Biotech. 5: 291–295.
Franzmann, P. D., and Tindall, B. J., 1990, A chemotaxonomic study of members of the family Halomonadaceae, Syst. Appl. Microbiol. 13: 142–147.
Galbraith, L., and Wilkinson, S. G., 1991, Polar lipids and fatty acids of Pseudomonas carophylli, Pseudomonas gladioli, and Pseudomonas pickettii, J. Gen. Microbiol. 137: 197–202.
Guckert, J. B., Ringelberg, D. B., and White, D. C., 1987, Biosynthesis of trans fatty acids from acetate inthe bacterium Pseudomonas atlantica, Can. J. Microbiol. 33: 748–754.
Hastie, A. T., Hingley, S. T., Higgins, M. L., Kueppers, F., and Shryok T., 1986, Rhamnolipid from Pseudomonas aeruginosa inactivates mammaliam trachéal ciliary axonemes, Cell. Motil. Cytoskeleton 6: 502–509.
Heipieper, H.-J., Deifenbach, R., and Keweloh, H., 1992, Conversion of cis unsaturated ratty acids to trans, a possible mechanism for the protection of phenol-degrading Pseudomonas P8 from substrate toxicity, Appl. Environ. Microbiol. 58: 1847–1852.
Heipieper, H.-J., and de Bont, J. A. M., 1994, Adaptation of Pseudomonas putida S12 to ethanol and toluene at the level of fatty acid composition of membranes, Appl. Environ. Microbiol. 60: 4440–4444.
Huijberts, G. N. M., DeRijk, T. C, de Waard, P., and Eggink G., 1994, 13C nuclear magnetic resonance studies of Pseudomonas putida fatty acid metabolic routes involved in poly(3-hydroxyalkoanate) synthesis, J. Bacteriol. 176: 1661–1666.
Huijberts, G. N. M., Eggink, G., de Waard, P., Huisman, G. W, and Witholt, B., 1992, Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkoa-nates) consisting of saturated and unsaturated monomers, Appl. Environ. Microbiol. 58: 536–544.
Jacques, N. A., 1981, Studies on cyclopropane fatty acid synthesis: Correlation between the state of reduction of respiratory components and the accumulation of méthylene hexadecanoic acid by Pseudomonas denitrificans, Biochim. Biophys. Acta 665: 270–282.
Jacques, N. A., and Hunt, A. L., 1989, Studies on cyclopropane fatty acid synthesis. Effect of carbon source and oxygen tension on cyclopropane fatty acid synthetase activity in Pseudomonas denitrificans, Biochim. Biphys. Acta 619: 453–470.
Janse, J. D., 1991, infra and intraspecific classification of Pseudomonas solanacearum strains, using whole-cell fatty acid analysis, Syst. Appl. Microbiol. 14: 335–345.
Janse, J. D., 1991, Pathovar discrimination within Psendomonas syringae subsp. savastanoi using whole cell fatty acids and pathogenicity as criteria, Syst. Appl. Microbiol. 14: 79–84.
Karunaratne, D. N., Richards, J. C, and Hancock, R. E. W., 1992, Characterization of Lipid A from Pseudomonas aeruginosa O-antigenic B-band lipopolysaccharide by 1D and 2D NMR and mass spectral analysis, Arch. Biochem. Biophys. 299: 268–376.
Kenward, M. A., Alcock, S. R., and Brown, M. R., 1980, Effects of hyperbaríc oxygen on the growth and properties of Pseudomonas aeruginosa, Microbios 28: 47–60.
Kharami, A., Bibi, Z., Neilson, H., Holby, N., and Doring, G., 1989, Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil and monocyte function, APMIS 97: 1–68–1072.
Kieft, T. L., Ringelberg, D. B., and White D. C, 1994, Changes in ester-linked fatty acid profiles of subsurface bacteria during starvation and dessication in a porous medium, Appl. Environ. Microbiol. 60: 3292–3299.
Kochi, M., Weiss, D. W, Pugh, L. H., and Groupe, V., 1951, Viscosin, a new antibiotic, Bact. Proc. 29-30.
Kropinski, A. M. B., Lewis, V., and Berry, D., 1987, Effect of growth temperature on the lipids, outer membrane proteins, and lipopolysaccharides of Pseudomonas aeruginosa PAO, J. Bacteriol. 169: 1960–1966.
Latifi, A., Winson, M. D., Foglino, M., Bycroft, B. W, Stewart, G. S., Lazdunski, A., and Williams, P., 1995, Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1, Mol. Microbiol. 17: 333–343.
Laycock, M. V., Hildebrand, P. D., Thibault, P., Walter, J. A., and Wright, J. L. C, 1991, Viscosin, a potent peptidolipid biosurfactant and phytopathogenic mediator produced by a pectolytic strain of Pseudomonas fluorescens, J. Agri. Food Chem. 39: 483–489.
Lee, E. Y., Jendrossek, D., Schirmer, A., Choi, C. Y., and Steinbuchel, A., 1995, Biosynthesis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1,3-butanediol or from 3-hydroxybutyrate by Pseudomonas sp. A33, Appl. Microbiol. Biotechnol. 42: 901–909.
Mayer, H., Krauss, J. H., Urbanik-Sypniewska, T., Puvanesarajah, V., Stacey, G., and Auling, G., 1989, Lipid A with 2,3-diamino-2,3-dideoxy-glucose in lipopolysaccharides from slow-growing members of Rhizobiaceae and “Pseudomonas carboxydovarans,” Arch. Microbiol. 151: 111–116.
Michea-Hamzehpour, M., Furet, Y. X., and Pechere, J.-C, 1991, Role of protein D2 and lipopolysaccharide in diffusion of quinolones through the outer membrane of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 35(10): 2091–2097.
Minnikin, D. E., and Abdolrahimzadeh, H., 1974, The replacement of phosphatidylethanolamine and acidic phospholipids by an ornithine-amide lipid and a minor phosphorus-free lipid in Pseudomonas fluorescens NCMB 129, FEBS Lett. 43: 257–260.
Monteoliva-Sanchez, M., and Ramos-Cormenzana, A., 1987, Cellular fatty acid composition in moderately halophilic gram-negative rods, J. Appl. Bacteriol. 62: 361–366.
Neu, T. R., Hartner, T., and Poralla, K., 1990, Surface active properties of viscosin: A peptidolipid antibiotic, Appl. Microbiol. Biotechnol. 32: 518–520.
Norris, M. J., Rogers, D. T., and Russell, A. D., 1985, Cell envelope composition and sensitivity of Proteus Mirabilus, Pseudomonas aeruginosa, and Serratia marcescens to polymixin and other antibacterial agents, Lett. Appl. Microbiol. 1: 3–6.
Ochsner, U. A., and Reiser, J., 1995, Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. USA 92: 6424–6428.
Passador, L., Cook, J. M., Gambello, M. J., Rust, L., and Iglewski, B. H., 1993, Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication, Science 260: 1127–1130.
Pearson, J. P., Passador, L., Iglewski, B. H., and Greenberg, E. P., 1995, A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. USA 92: 1490–1494.
Pinkart, H. C, Wolfram, J., Rogers, R., and White D. C, 1995, Cell envelope changes in solvent-tolerant and solvent-sensitive Pseudomonas putida strains following exposure to o-xylene, Appl. Environ. Microbiol. 62: 1129–1132.
Preusting, H., Kingma, J., Huisman, G. W, Steinbuchel, A., and Witholt, B., 1992, Formation of polyester blends by a recombinant strain of Pseudomonas oleovorans: Different poly(3-hydroxyalkoantes) are stored in separate granules, J. Environ. Polym. Degradation 1: 11–21.
Rendell, N. B., Taylor, G. W, Somerville, M., Todd, H., Wilson, R., and Cole, P. J., 1990, Characterization of Pseudomonas rhamnolipids, Biochim. Biophys. Acta 1045: 189–193.
Rosello-Mora, R. A., Lalucat, J., Dott, W., and Kampfer, P., 1994, Biochemical and chemotaxonomic characterization of Pseudomonas stutzen genomovars, J. Appl. Bacteriol. 76: 226–233.
Roussel, J. and Asselineau, J., 1980, Fatty acid composition of the lipids of Pseudomonas mildenbergii: Presence of a fatty acid containing two conjugated double bonds, Biochim. Biophys. Acta 619: 689–692.
Segers, P., Vancanneyt, M., Pot, B., Torck, U., Hoste, B., Dewettinck, D., Falsen, E., Kersters, K., and de Vos, P., 1994, Classification of Pseudomonas diminuta (Leifson and High 1954) and Pseudomonas vesicularis (Busing, Doll and Freytag 1953) in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively, Int. J. Syst. Bacteriol. 44: 499–510.
Sikkema, J., Weber, F. J., Heipieper, H. J., and de Bont, J. A. M., 1994, Cellular toxicity of lipophilic compounds: Mechanisms, implications, and adaptations, Biocatalysis 10: 113–122.
Sikkema, J., de Bont, J. A. M., and Poolman, B., 1995, Mechanisms of membrane toxicity of hydrocarbons, Microbiol. Rev. 59: 201–222.
Somerville, M., Taylor, G. W, Watson, D., Rendell, N. B., Rutman, A., Todd, H., Davies, J. R., Wilson, R., Cole, P., and Richardson, P. S., 1992, Release of mucus glycoconjugates by Pseudomonas aeruginosa rhamnolipid into feline trachea in vivo and human bronchus in vitro, Am. J. Respir. Cell. Mol. Biol. 6: 116–122.
Stead, D. E., 1992, Grouping of plant-pathogenic and some other Pseudomonas spp. by using cellular fatty acid profiles, Int. J. Syst. Bacteriol. 42: 281–295.
Steinbuchel, A., Hustede, E., Leibergesell, M., Pieper, U., Timm, A., and Valentin, H., 1992, Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria, FEMS Microbiol. Rev. 103: 217–230.
Syldatk, C, Lang, S., Matulovik, U., and Wagner, F., 1985, Production of four interfacial active rhamnolipids from N-alkanes or glycerol by resting cells of Pseudomonas species DSM 2874, Z. Naturforsch. 40: 61–67.
Takeuchi, M., Sawada, W, Oyaizu, H., and Yolota, A., 1994, Phylogenetic evidence for Sphingomonas and Rhizomonas as nonphotosynthetic members of the alpha-4 subclass of the Proteobacteria, Int. J. Syst. Bacteriol. 44: 308–314.
Taylor, C. J., Carrick, B. J., Galbraith, L., and Wilkinson, S. G., 1993, Polar lipids of Pseudomonas diazotrophicus, FEMS Microbiol. Lett. 106: 65–70.
Timm, A., and Steinbuchel, A., 1992, Cloning and molecular analysis of the poly(3-hy-droxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1, FEBS Eur. J. Biochem. 209: 15–30.
Van Dyke, M. W., Couture, P., Brauer, M., Lee, H., and Trevors, J. T., 1993, Pseudomonas aeruginosa UG2 rhamnolipid biosurfactants: Structural characterization and their use in removing hydrophobic compounds from soil, Can. J. Microbiol. 39: 1071–1078.
Wada, M., Fukunaga, N., and Sasaki, S., 1987, Effect of growth temperature on phospholipid and fatty acid composition in a phychrotrophic bacterium, Pseudomonas sp. strain E-3, Plant Cell. Physiol. 28: 1209–1217.
Weber, F. J., Isken, S., and de Bont, J. A. M., 1994, Cis/trans isomerization of fatty acids as a defense mechanism of Pseudomonas putida strains to toxic concentrations of toluene, Microbiology 140: 2013–2017.
White, D. C, Sutton, S. D., and Ringleberg, D. B., 1996, The genus Sphingomonas: Physiology and ecology, Current Opinion in Biotechnology, July.
Wilkinson, S. G., 1988, Gram-negative bacteria, in: Microbial Lipids (C. Ratledge and S. G. Wilkinson eds.), Academic Press, San Diego, Vol. 1, pp. 333–348.
Wilkinson, S. G., Galbraith, L., and Lightfoot, G. A., 1973, Cells walls, lipids, and lipopolysaccharides of Pseudomonas species, Eur. J. Biochem. 33: 158–174.
Winson M. K., Camara, M., Latifi, A., Foglino, M., Chabra, S. R., Daykin, M., Bally, M., Chapon, V, Salmond, G. P., and Bycroft, B. W, 1995, Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. USA 92: 9427–9431.
Yabuuchi, E., Yano, I., Oyaizu, H., Hashimoto, Y., Ezaki, T., and Yamamoto, Y, 1990, Proposals of Sphingomonas paucimobilis gen. nov. and comb., nov. Sphingomonas parapaucimobilis sp. Nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb, nov., and two genospecies of the genus Sphingomonas, Microbiol. Immunol. 34: 99–119.
Yabuuchi, E., Kosako, Y., Arakawa, M., Hotta, H., and Yano, I., 1992, Identification of Oklahoma isolate as a strain of Pseudomonas pseudomallei, Microbiol. Immunol. 36: 1239–1249.
Yabuuchi, E., Kosaka, Y., Oyaizu, H., Yano, I., Hotta, H., Hashimoto, H., Ezaki, T., and Arakawa, M., 1994, Proposal of Burkholderia gen. nov. and transfer of seven species of the Pseudomonas hoimology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb, nov., Microbiol. Immunol. 36: 1251–1275.
Zhang, Y, and Miller, R. M., 1992, Enhanced octadecane dispersion and biodégradation by a Pseudomonas rhamnolipid (biosurfactant), Appl. Env. Microbiol. 58: 3276–3282.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Pinkart, H.C., White, D.C. (1998). Lipids of Pseudomonas . In: Montie, T.C. (eds) Pseudomonas. Biotechnology Handbooks, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0120-0_4
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0120-0_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0122-4
Online ISBN: 978-1-4899-0120-0
eBook Packages: Springer Book Archive