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Selective Systems in Somatic Cell Genetics

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Advances in Human Genetics

Part of the book series: Advances in Human Genetics ((AHUG,volume 7))

Abstract

Experimental mutagenesis, cell hybridization, and intercellular gene transfer appear to be the three most significant technical developments in recent years that have stimulated rapid advances in somatic cell genetics. The ability to induce mutations in animal and plant somatic cells in culture enriches the genetic variability of the cell populations for further genetic and biochemical analysis. The process of mutagenesis and mutation rates in high eukaryotic cells can thus be studied and compared with those known in prokaryotes. The success of cell fusion between diverse parental genomes into multinucleate heterokaryons or synkaryonic hybrids has also led to a variety of investigative possibilities, among which are the studies of genomic interactions and gene expression, genetic complementation, recombination, segregation, and mapping of genes. However, parasexual transfer of genetic material from one cell to another is not limited to the process of fusion between intact cells. Successful attempts have been reported in which isolated DNA, chromosomes, or nuclear fragments have been introduced into living mammalian somatic cells. The expression of heterologous gene(s) in a recipient cell and the transmission of the “acquired” character (s) to cell progeny are problems of fundamental interest and significance.

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References

  1. Adhya, S., and Schwartz, M., 1971, Phosphoglucomutase mutants of Escherichia coli K12, J. Bacteriol. 108: 621–626.

    CAS  PubMed Central  PubMed  Google Scholar 

  2. Adler, H. I., Fisher, W. D., Cohen, A., and Hardigree, A. A., 1966, Miniature Escherichia coli cells deficient in DNA, Proc. Natl. Acad. Sci. U.S.A. 57: 321–326.

    Google Scholar 

  3. Adoutte, A., and Beissen, J., 1970, Cytoplasmic inheritance of erythromycin resistant mutations in Paramecium aurelia, Mol. Gen. Genet. 108: 70–77.

    CAS  PubMed  Google Scholar 

  4. Albertini, R. J., and DeMars, R., 1970, Diploid azaguanine resistant mutants of cultured human fibroblasts, Science 169: 482–485.

    CAS  Google Scholar 

  5. Albertini, R. J., and DeMars, R., 1973, Somatic cell mutation. Detection and quantification of X-ray-induced mutation in cultured, diploid human fibroblasts, Mutat. Res. 18: 199–224.

    CAS  PubMed  Google Scholar 

  6. Albrecht, A. M., Biedler, J. L., and Hutchison, D. J., 1972, Two different species of dihydrofolate reductase in mammalian cells differentially resistant to amethopterin and methasquin, Cancer Res. 32: 1539–1546.

    CAS  PubMed  Google Scholar 

  7. Arlett, C. F., and Harcourt, S. A., 1972, The induction of 8-azaguanine-resistant mutants in cultured Chinese hamster cells by ultraviolet light. The effect of changes in post-irradiation conditions, Mutat. Res. 14: 431–437.

    CAS  PubMed  Google Scholar 

  8. Arlett, C. F., and Harcourt, S. A., 1972, Expression time and spontaneous mutability in the estimation of induced mutation frequency following treatment of Chinese hamster cells by ultraviolet light, Mutat. Res. 16: 301–306.

    CAS  PubMed  Google Scholar 

  9. Atkins, J. H., and Gartler, S. M., 1968, Development of a nonselective technique for studying 2,6,diaminopurine resistance in an established murine cell line, Genetics 60: 781–792.

    CAS  PubMed Central  PubMed  Google Scholar 

  10. Barski, G., 1973, Somatic hybridization in studies of heredity of cell malignancy, in “Tissue Culture. Methods and Application” (P. F. Kruse, Jr. and M. K. Patterson, Jr., eds.), pp. 469–475, Academic Press, New York.

    Google Scholar 

  11. Barski, G., Sorieul, S., and Cornefert, F., 1960, Production dans des cultures in vitro de deux souches cellulaires en association, de cellules de caractére “hybrid”, C. R. Acad. Sci. 251: 1825–1827.

    CAS  Google Scholar 

  12. Basilico, C., and Meiss, H. K., 1974, Methods for selecting and studying temperature-sensitive mutants of BHK-21 cells, in “Methods in Cell Biology” Vol. 8 (D. M. Prescott, ed.), pp. 1–22, Academic Press, New York.

    Google Scholar 

  13. Beadle, G. W., and Tatum, E. L., 1945, Neurospora II. Methods of producing and detecting mutants concerned with nutritional requirements, Am. J. Bot. 32: 678–686.

    Google Scholar 

  14. Beale, G. H., 1969, A note on the inheritance of erythromycin-resistance in Paramecium aurelia, Genet. Res. 14: 341–342.

    CAS  PubMed  Google Scholar 

  15. Beaudet, A. L., Roufa, D. J., and Caskey, C. T., 1973, Mutations affecting the structure of hypoxanthine-guanine phosphoribosyltransferase in cultured Chinese hamster cells, Proc. Natl. Acad. Sci. U.S.A. 70: 320–324.

    CAS  PubMed Central  PubMed  Google Scholar 

  16. Bendich, A., Borenfreund, E., and Steinberg, S., 1974, Penetration of somatic mammalian cells by sperm, Science 183: 857–859.

    CAS  PubMed  Google Scholar 

  17. Bennett, L. L., Vail, M. H., Chumley, S., and Montgomery, J. A., 1966, Activity of adenosine analogs against a cell culture line resistant to 2-fluoroadenine, Biochem. Phamacol. 15: 1719–1728.

    CAS  Google Scholar 

  18. Benzer, S., and Freese, E., 1958, Induction of specific mutations with 5-bromouracil, Proc. Natl. Acad. Sci. U.S.A. 44: 112–119.

    CAS  PubMed Central  PubMed  Google Scholar 

  19. Beutler, E., and Yoshida, A., 1973 Human glucose-6-phosphate dehydrogenase variants: a supplementary tabulation, Ann. Hum. Genet. 37: 151–156.

    CAS  PubMed  Google Scholar 

  20. Bolotin, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Petrochilo, E., and Solnimski, P., 1971, La recombinaison des mitochondries chez Saccharooz ces cerevisiae, Bull. Inst. Pasteur Paris 69: 215–239.

    Google Scholar 

  21. Boone, C., Chen, T. R., and Ruddle, F. H., 1972, Assignment of three human genes to chromosomes (LDH-A to 11; TK to 17; and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids, Proc. Natl. Acad. Sci. U.S.A. 69: 510–514.

    CAS  PubMed Central  PubMed  Google Scholar 

  22. Borst, P., 1972, Mitochondria] nucleic acids, Anna. Rev. Biochem. 41: 333–376.

    CAS  Google Scholar 

  23. Boume, H. R., Coffino, P., Melmon, K. L., Tomkins, G. M.. and Weinstein. Y., 1975, Genetic analysis of cyclic AMP in a mammalian cell, in “Advances in Cyclic Nucleotide Research” Vol. 5 (G. I. Drummond, P. Greengard, and G. A. Robinson, eds.), pp. 771–786, Raven Press, New York.

    Google Scholar 

  24. Bourne, H. R., Coffino, P., and Tomkins, G. M.. 1975, Selection of a variant lymphoma cell deficient in adenylate cyclase, Science 187: 750–752.

    CAS  PubMed  Google Scholar 

  25. Bourne, H. R., Coffino, P., and Tomkins, G. M., 1975, Somatic genetic analysis of cyclic AMP action: Characterization of unresponsive mutants, J. Cell. Physiol. 85: 611–620.

    CAS  PubMed  Google Scholar 

  26. Boume, H. R., Tomkins, G. M., and Dion, S., 1973, Regulation of phosphodiesterase synthesis: requirement for cyclic adenosine monophosphate-dependent protein kinase, Science 181: 952–954.

    Google Scholar 

  27. Boyd, Y. L., and Harris, H., 1973, Correction of genetic defects in mammalian cells by the input of small amounts of foreign genetic material, J. Cell Sci. 13: 841–861.

    CAS  PubMed  Google Scholar 

  28. Brenner, M., and Ames, B. N., 1967, The histidine operon and its regulation, in “Metabolic Pathways,” Vol. 5 (H. J. Vogel, ed.), pp. 349–387, Academic Press, New York.

    Google Scholar 

  29. Bridges, B. A., and Huckle, J., 1970, Mutagenesis of cultured mammalian cells by X-radiation and ultraviolet light, Mutat. Res. 10: 141–151.

    CAS  PubMed  Google Scholar 

  30. Bunn, C. L., Mitchell, C. H., Lukins, H. B., and Linnane, A. W., 1970, Biogenesis of mitochondria, XVIII. A new class of cytoplasmically determined antibiotic resistant mutants in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. U.S.A. 67: 1233–1240.

    CAS  PubMed Central  PubMed  Google Scholar 

  31. Bunn, C. L., Wallace, D. C., and Eisenstadt, J. M., 1974, Cytoplasmic inheritance of chloramphenicol resistance in mouse tissue culture cells, Proc. Natl. Acad. Sci. U.S.A. 71: 1681–1685.

    CAS  PubMed Central  PubMed  Google Scholar 

  32. Burch, J. W., and McBride, O. W., 1975, Human gene expression in rodent cells after uptake of isolated metaphase chromosomes, Proc. Natl. Acad. Sci. U.S.A. 72: 1797–1801.

    CAS  PubMed Central  PubMed  Google Scholar 

  33. Burkholder, G. D., and Mukherjee, B. B., 1970, Uptake of isolated metaphase chromosomes by mammalian cells in vitro, Exp. Cell Res. 61: 413–422.

    CAS  PubMed  Google Scholar 

  34. Cairns, J., and deLucia, P., 1969, Isolation of an E. coli strain with a mutation affecting DNA polymerase, Nature 224: 1164–1166.

    PubMed  Google Scholar 

  35. Cavalli-Sforza, L. L., and Lederberg, J., 1956, Isolation of preadaptive mutants in bacteria by sib selection, Genetics 41: 367–381.

    Google Scholar 

  36. Chan, T.-S., Long, C., and Green, H., 1975, A human—mouse somatic cell hybrid line selected for human deoxycytidine deaminase, Somatic Cell Genet. 1: 81–90.

    CAS  PubMed  Google Scholar 

  37. Chan, V. L., Whitmore, G. F., and Siminovitch, L., 1972, Mammalian cells with altered forms of RNA polymerase II, Proc. Natl. Acad. Sci. U.S.A. 69: 3119–3123.

    CAS  PubMed Central  PubMed  Google Scholar 

  38. Chang, R. S., Liepins, H., and Margolish, M., 1961, Carbon dioxide requirement and nucleic acid metabolism of HeLa and conjunctival cells, Proc. Soc. Exp. Biol. Med. 106: 149–152.

    CAS  PubMed  Google Scholar 

  39. Chasin, L. A., 1974, Mutations affecting adenine phosphoribosyl transferase activity in Chinese hamster cells, Cell 2: 37–41.

    CAS  PubMed  Google Scholar 

  40. Chasin, L. A., Feldman, A., Konstam, M., and Urlaub, G., 1974, Reversion of a Chinese hamster cell auxotrophic mutant, Proc. Natl. Acad. Sci. U.S.A. 71: 718–722.

    CAS  PubMed Central  PubMed  Google Scholar 

  41. Choi, K. W., and Bloom, A. D., 1970, Clóning of human lymphocytes in vitro, Nature 227: 171–173

    CAS  PubMed  Google Scholar 

  42. Choräzy, M., Bendich, A., Borenfreund, E., Ittensohn, O. L., and Hutchinson, D. J., 1963, Uptake of mammalian chromosomes by mammalian cells, J. Cell Biol. 19: 71–77.

    PubMed Central  Google Scholar 

  43. Chu, E. H. Y., 1971, Mammalian cell genetics, III. Characterization of X-ray-induced forward mutations in Chinese hamster cell cultures, Mutat. Res. 11: 23–34.

    CAS  PubMed  Google Scholar 

  44. Chu, E. H. Y., 1971, Induction and analysis of gene mutations in mammalian cell cultures, in “Chemical Mutagens,” Vol. 2, (A. Hollaender, ed.), pp. 411–444, Plenum Press, New York.

    Google Scholar 

  45. Chu, E. H. Y., 1974, Induction and analysis of gene mutations in cultured mammalian somatic cells, Genetics 78: 115–132.

    CAS  PubMed Central  PubMed  Google Scholar 

  46. Chu, E. H. Y., Brimer, P., Jacobson, K. B., and Merriam, E. V., 1969, Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for Lglutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro, Genetics 62: 359–377.

    CAS  PubMed Central  PubMed  Google Scholar 

  47. Chu, E. H. Y., Brimer, P. A., Schenley, C. K., Ho, T., and Mailing, H. V., 1974, Reversion studies of chemically-induced mutants in Chinese hamster cells, in “Molecular and Environmental Aspects of Mutagenesis” (L. Prakash, F. Sherman, M. W. Miller, C. W. Lawrence, and H. W. Taper, eds.), pp. 178–195, Charles C. Thomas, Springfield.

    Google Scholar 

  48. Chu, E. H. Y., and Mailing, H. V., 1968, Chemical mutagenesis in Chinese hamster cells in vitro, in “Proc. 12th Intl. Congr. Genetics,” Vol. I, p. 102, Science Council of Japan, Tokyo.

    Google Scholar 

  49. Chu, E. H. Y., and Mailing, H. V., 1968, Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro, Proc. Natl. Acad. Sci. U.S.A. 61: 1306–1312.

    CAS  PubMed Central  PubMed  Google Scholar 

  50. Chu, E. H. Y., Sun, N. C., and Chang, C. C., 1972, Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light, Proc. Natl. Acad. Sci. U.S.A. 69: 3459–3463.

    CAS  PubMed Central  PubMed  Google Scholar 

  51. Chu, E. H. Y., Sun, N. C., and Chang, C. C., 1975, Genetic markers associated with hamster chromosomes, in “Mammalian Cells: Problems and Probes” (C. R. Richmond, D. F. Peterson, P. P. Mullaney, and E. C. Anderson, eds.), pp. 228–238, National Technical Information Service, U. S. Dept. of Commerce, Springfield.

    Google Scholar 

  52. Chu, M. Y., and Fischer, G. A., 1965, Comparative studies of leukemic cells sensitive and resistant to cytosine arabinoside, Biochem. Pharmacol. 14: 333–341.

    CAS  PubMed  Google Scholar 

  53. Clausen, R. E., and Cameron, D. R., 1957, Inheritance in Nicotiana tabacum. XXVIII. The cytogenetics of introgression, Proc. Natl. Acad. Sci. U.S.A. 43: 908–913.

    CAS  PubMed Central  PubMed  Google Scholar 

  54. Coffino, P., Bourne, H. R., and Tomkins, G. M., 1975, Somatic genetic analysis of cyclic AMP action: Selection of unresponsive mutants, J. Cell. Physiol. 85: 603–610.

    CAS  PubMed  Google Scholar 

  55. Coffino, P., Gray, J. W., and Tomkins, G. M., 1975, Cyclic AMP, a nonessential regulator of the cell cycle, Proc. Natl. Acad. Sci. U.S.A. 73: 878–882.

    Google Scholar 

  56. Coffino, P., Laskov, R., and Scharff, M. D., 1970, Immunoglobulin production: methods for quantitatively detecting variant myeloma cells, Science 167: 186–188.

    CAS  PubMed  Google Scholar 

  57. Cox, R. P., Krauss, M. R., Balis, M. E., and Dancis, J., 1972, Communication between normal and enzyme deficient cells in tissue culture, Exp. Cell Res. 74: 251–268.

    CAS  PubMed  Google Scholar 

  58. Creagan, R. P., Chen, S., and Ruddle, F. H., 1975, Genetic analysis of the cell surface, association of human chromosome 5 with sensitivity to diphtheria toxin of mouse-human somatic cell hybrids, Proc. Natl. Acad. Sci. U.S.A. 72: 2237–2241.

    CAS  PubMed Central  PubMed  Google Scholar 

  59. Creasey, W. A., 1963, Studies on the metabolism of 5’-iodo-2’-deoxycytidine in vitro, J. Biol. Chem. 238: 1772–1776.

    CAS  PubMed  Google Scholar 

  60. Daniel, V., Litwack, G., and Tomkins, G., 1973, Induction of cytolysis of cultured lymphoma cells by adenosine 3’,5’ cyclic monophosphate and the isolation of resistant variants, Proc. Natl. Acad. Sci. U.S.A. 70: 76–79.

    CAS  PubMed Central  PubMed  Google Scholar 

  61. Davidson, R. L., Adelstein, S. J., and Oxman, M. N., 1973, Herpes simplex virus as a source of thymidine kinase for thymidine kinase-deficient mouse cells: Suppression and reactivation of the viral enzyme, Proc. Natl. Acad. Sci. U.S.A. 70: 1912–1916.

    CAS  PubMed Central  PubMed  Google Scholar 

  62. Davidson, R. L., and de la Cruz, F. F. (eds), 1974, “Somatic Cell Hybridization,” Raven Press, New York.

    Google Scholar 

  63. Davidson, R. L. and Ephrussi, B., 1965, A selective system for the isolation of hybrids between L cells and normal cells, Nature 205: 1170–1171.

    Google Scholar 

  64. Davis, B. D., 1948, Isolation of biochemically deficient mutations of bacteria by penicillin, J. Am. Chem. Soc. 70: 4267.

    CAS  PubMed  Google Scholar 

  65. DeLuca, C., and Gioelli, R. P., 1971, Transhydrogenase activity in mammalian cells in vitro: Its possible physiological significance, In Vitro 7: 13–16.

    CAS  Google Scholar 

  66. DeMars, R., 1968, A temperature sensitive glucose-6-phosphate dehydrogenase in mutant cultured human cells, Proc. Natl. Acad. Sci. U.S.A. 61: 562–569.

    CAS  PubMed Central  PubMed  Google Scholar 

  67. DeMars, R., 1974, Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection, Mutat. Res. 24: 335–364.

    CAS  PubMed  Google Scholar 

  68. DeMars, R., and Hooper, J. L., 1960, A method of selecting for auxotrophic mutants of HeLa cells, J. Exp. Med. 111: 559–573.

    CAS  Google Scholar 

  69. De Saint Vincent, B. P., and Buttin, G., 1973, Studies on 1-ß-o-arabinofuranosylcytosine resistant mutants of Chinese hamster fibroblasts, Eur. J. Biochem. 37: 481–488.

    Google Scholar 

  70. deSerres, F. J., 1964, Genetic analysis of the structure of the ad-3 region of Neurospora crassa by means of irreparable recessive lethal mutations, Genetics 50: 21–30.

    CAS  PubMed  Google Scholar 

  71. Ditta, G., Soderberg, K., Landy, F., and Scheffler, I. E., 1976, The selection of Chinese hamster cells deficient in oxidative energy metabolism, Somatic Cell Genet.,in press.

    Google Scholar 

  72. Drake. J. W., 1970, “The Molecular Basis of Mutation,” Holden-Day, San Francisco.

    Google Scholar 

  73. Duncan, M. E., and Brookes, P., 1973, The induction of azaguanine-resistant mutants in cultured Chinese hamster cells by reactive derivatives of carcinogenic hydrocarbons, Mutat. Res. 21: 107–118.

    CAS  PubMed  Google Scholar 

  74. Ebina, T., Karmo, I., Takahashi, K., Homma, M., and Ishida. N., 1970, Incorporation of isolated HeLa-S3 metaphase chromosomes into cultured mouse embryo cells, Exp. Cell Res. 62: 384–388.

    CAS  PubMed  Google Scholar 

  75. Edgar, R. S., and Lielausis, I., 1964, Temperature sensitive mutants of bacteriophage T4D: Their isolation and genetic characterization, Genetics 49: 649–662.

    CAS  PubMed Central  PubMed  Google Scholar 

  76. Ege, T., Hamburg, H., Krondahl, U., Ericsson, J., and Ringertz, N. R., 1974, Characterization of mini-cells (nuclei) obtained by cytochalasin enucleation, Exp. Cell Res. 87: 365–376.

    CAS  PubMed  Google Scholar 

  77. Ege, T., and Ringertz, N. R., 1974, Preparation of micro-cells by enucleation of micronucleate cells, Exp. Cell Res. 87: 378–382.

    CAS  PubMed  Google Scholar 

  78. Eisenstark, A., 1971, Mutagenic and lethal effects of visible and near-ultraviolet light on bacterial cells, Adv. Genet. 16: 167–198.

    CAS  PubMed  Google Scholar 

  79. Elsevier, S. M., Kucherlapati, R. S., Nichols, E. A., Willecke, K., Creagan, R. P., Giles, R. E., McDougall, J. K., and Ruddle, F. H., 1975, Assignment and regional localization of a gene coding for galactokinase to human chromosome 17g21–22, Cytogenet. Cell Genet. 14: 117–119.

    Google Scholar 

  80. Engelsberg, E., and Bass, R., 1974, Inhibition of the growth of L cells by the natural amino acids and isolation and characterization of resistant mutants, In Vitro 10: 385.

    Google Scholar 

  81. Enbelsberg, E., and Ingraham, L., 1957, Meiotrophic mutants of Pasteurella pestis and their use in elucidation of nutritional requirements, Proc. Natl. Acad. Sci. U.S.A. 43: 369–373.

    Google Scholar 

  82. Ephrussi, B., 1972, “Hybridization of Somatic Cells,” Princeton University Press, Princeton.

    Google Scholar 

  83. Ephrussi, B., and Sorieul, S., 1962, Nouvelles observations sur l’hybridization “in vitro” de cellules de souris, C. R. Acad. Sci. 254: 181–182.

    CAS  Google Scholar 

  84. Ephrussi, B., and Weiss, M. C., 1965, Interspecific hybridization of somatic cells, Proc. Natl. Acad. Sci. U.S.A. 53: 1040–1042.

    CAS  PubMed Central  PubMed  Google Scholar 

  85. Epstein, R. H., Bolle, A., Steinberg, C. M., Kellenberger, E., Boy de la Tour, E., Chevalley, R., Edgar, R. S., Susman, M., Denhardt, G. H., and Lielausis, I., 1963. Physiological studies of conditional lethal mutants of bacteriophage T4D, Cold Spring Harbor Symp. Quant. Biol. 28: 375–394.

    CAS  Google Scholar 

  86. Farber, F., Melnick, J., and Butel, J., 1975, Optimal conditions for uptake of exogenous DNA by Chinese hamster cells deficient in hypoxanthine-guanine phosphoribosyltransferase, Biochim. Biophys. Acta 390: 298–311.

    CAS  PubMed  Google Scholar 

  87. Fenwick, R. G., Jr., and Caskey, C. T., 1975, Mutant Chinese hamster cells with a thermosensitive hypoxanthine-guanine phosphoribosyltransferase, Cell 5: 115–122.

    CAS  PubMed  Google Scholar 

  88. Firkin, F. C., and Linnane, A. W., 1968, Differential effects of chloramphenicol on the growth and respiration of mammalian cells, Biochem. Biophys. Res. Commun. 32: 398–402.

    CAS  PubMed  Google Scholar 

  89. Fischer, G. A., Lee, S. Y., and Calabresi, P., 1974, Detection of chemical mutagens using a host-mediated assay (L5178Y) mutagenesis system, Mutat. Res. 26: 501–511.

    CAS  PubMed  Google Scholar 

  90. Fisher, H. W., and Puck, T. T., 1956, On the functions of X-irradiated “feeder” cells in supporting growth of single mammalian cells, Proc. Natl. Acad. Sci. U.S.A. 42: 900–906.

    CAS  PubMed Central  PubMed  Google Scholar 

  91. Fox, A., and Yoon, S. B., 1970, DNA-induced transformation in Drosophila: Locus specificity and the establishment of transformed stocks, Proc. Natl. Acad. Sci. U.S.A. 67: 1608–1615.

    CAS  PubMed Central  PubMed  Google Scholar 

  92. Fox, M., 1975, Factors affecting the quantitation of dose-response curves for mutation induction in V-79 Chinese hamster cells after exposure to chemical and physical mutagens, Mutat. Res. 29: 449–466.

    CAS  PubMed  Google Scholar 

  93. Fox, M., Fox, B. W., and Ayad, S. R., 1969, Evidence for genetic expression of integrated DNA in lymphoma cells, Nature 222: 1086–1087.

    CAS  PubMed  Google Scholar 

  94. Fraenkel, D. G., and Levisohn, S. R., 1967, Glucose and gluconate metabolism in Escherichia coli: A study of a mutant lacking phosphoglucose isomerase, J. Bacteriol. 93: 1571–1578.

    CAS  PubMed Central  PubMed  Google Scholar 

  95. Fraenkel, D. G., Osborn, M. J., Horecker, B. L., and Smith, S. M., 1963, Metabolism and cell wall structure of a mutant of Salmonella typhimurium deficient in phosphoglucose isomerase, Biochem. Biophys. Res. Commun. 11: 423–428.

    CAS  PubMed  Google Scholar 

  96. Fraenkel, D. G., and Vinopal, R. T., 1973, Carbohydrate metabolism in bacteria, Annu. Rev. Microbiol. 27: 69–100.

    CAS  Google Scholar 

  97. Franks, D., 1968, Antigens as markers on cultured mammalian cells, Biol. Rev. 43: 17–50.

    CAS  PubMed  Google Scholar 

  98. Fratatoni, J. C., Hall, C. W., and Neufeld, E. F., 1968, Hurler and Hunter syndromes: Mutual correction of the defect in cultured fibroblasts, Science 162: 570–572.

    Google Scholar 

  99. Freese, E., 1963, Molecular mechanism of mutation, in “Molecular Genetics,” Part I (J. H. Taylor, ed.), pp. 207–269, Academic Press, New York.

    Google Scholar 

  100. Fries, N., 1947, Experiments with different methods of isolating physiological mutations of filamentous fingi, Nature 159: 199.

    CAS  PubMed  Google Scholar 

  101. Fulder, S. J., and Holliday, R., 1975, A rapid rise in cell variants during the sensence of populations of human fibroblasts, Cell 6: 67–73.

    CAS  PubMed  Google Scholar 

  102. Gale, G. R., Ostrander, W. E., and Atkins, L. M., 1968, Effects of alanosine on purine and pyrimidine synthesis, Biochem. Pharmacol. 17: 1823–1832.

    CAS  PubMed  Google Scholar 

  103. Gale, G. R., and Schmidt, G. B., 1968, Mode of action of alanosine, Biochem. Pharmacol. 17: 363–368.

    CAS  PubMed  Google Scholar 

  104. Gatlin, J. I., and Rosenthal, A. S., 1974, The regulatory role of divalent cations in human granulocyte chemotaxis: Evidence for an association between calcium exchanges and microtubule assembly, J. Cell Biol. 62: 594–609.

    Google Scholar 

  105. Gartler, S. M., Gandini, E. G., and Ceppellini, R., 1962, Glucose-6-phosphate dehydrogenase deficient mutant in human cell culture, Nature 193: 602–603.

    CAS  PubMed  Google Scholar 

  106. Gartler, S. M., and Pious, D. A., 1964, Genetics of mammalian cell cultures, Humangenetik 2: 83–114

    Google Scholar 

  107. Geyer, R. P., and Neimark, J. M., 1958, Response of COL-deficient human cells in vitro to normal cell extracts, Proc. Soc. Exp. Biol. Med. 99: 599–601.

    CAS  PubMed  Google Scholar 

  108. Gilbert, S. F., and Migeon, B. R., 1975, D-Valine as a selective agent for normal human and rodent epithelial cells in culture, Cell 5: 11–17.

    CAS  PubMed  Google Scholar 

  109. Giles, R. E., and Ruddle, F. H., 1973, Production and characterization of proliferating somatic cell hybrids, in “Tissue Culture Methods and Applications” (P. F. Kruse, Jr. and M. K. Patterson, Jr., eds.), pp. 475–500, Academic Press, New York.

    Google Scholar 

  110. Gilula, N. B., Reeves, O. R., and Steinbach, A., 1972, Metabolic coupling, ionic coupling, and cell contacts, Nature 235: 262–265.

    CAS  PubMed  Google Scholar 

  111. Ginsberg, E., Salomon, D., Sreevalsan, T., and Freese, E., 1973, Growth inhibition and morphological changes caused by lipophilic acids in mammalian cells, Proc. Natl. Acad. Sci. U.S.A. 70: 2457–2461.

    Google Scholar 

  112. Glick, J. L., aid Salim, A. P., 1967, DNA-induced pigment production in a hamster cell line, J. Cell Biol. 33: 209–212.

    CAS  PubMed Central  PubMed  Google Scholar 

  113. Goldsby, R. A., and Zipser, E., 1969, The isolation and replica plating of mammalian cell clones, Exp. Cell Res. 54: 271–275.

    CAS  PubMed  Google Scholar 

  114. Goldstein, S., and Lin, C. C., 1972, Survival and DNA repair of somatic cell hybrids after ultraviolet irradiation, Nature (London) New Biol. 239: 142–145.

    CAS  Google Scholar 

  115. Gorini, L., 1970, Informational suppression, Annu. Rev. Genet. 4: 107–134.

    CAS  PubMed  Google Scholar 

  116. Green, C. D., and Martin, D. W.. Jr., 1973, Characterization of a feedback resistant phsophoribosylpyrophosphate synthetase from cultured, mutagenized hepatoma cells that overproduce purines, Proc. Natl. Acad. Sci. U.S.A. 70: 3698–3702.

    CAS  PubMed Central  PubMed  Google Scholar 

  117. Green, H., and Kehinde, O., 1974, Sublines of mouse 3T3 cells that accumulate lipid, Cell 1: 113–1 16.

    Google Scholar 

  118. Greer, H., and Fink, G. R., 1975, Isolation of regulatory mutants in Sacchromyces cerevisiae, in “Methods in Cell Biology,” Vol. 11 (D, M. Prescott, ed.), pp. 247272, Academic Press, New York.

    Google Scholar 

  119. Grzeschik, K.-H., 1973, Utilization of somatic cell hybrids for genetic studies in man, Humangenetik 19: 1–40.

    CAS  PubMed  Google Scholar 

  120. Hakala, M. T., 1957, Prevention of toxicity of amethopterin for sarcoma-180 cells in tissue culture, Science 126: 255.

    CAS  PubMed  Google Scholar 

  121. Ham, R. C., and Puck, T. T., 1962, Quantitative clonal growth of isolated mammalian cells, in “Methods in Enzymology.” Vol. 5 (S. P. Colowick and N. O. Kaplan, eds.), pp. 90–119, Academic Press, New York.

    Google Scholar 

  122. Handmaker, S. D., 1973, Hybridization of eukaryotic cells, Annu. Rev. Microbiol. 27: 189–204.

    CAS  PubMed  Google Scholar 

  123. Harris, H., 1974, “Nucleus and Cytoplasm.” Third Edition, Oxford University Press, London.

    Google Scholar 

  124. Harris, M., 1960, Specificity and cross-reactions in a strain of pig kidney cells resistant to 2,6-diaminopurine, Exp. Cell Res. 21: 439–442.

    CAS  PubMed  Google Scholar 

  125. Harris, M., 1964, “Cell Culture and Somatic Variation,” Holt, Rinehart, and Winston, New York.

    Google Scholar 

  126. Harris, M., 1971, Mutation rates in cells at different ploidy levels. J. Cell. Physiol. 78: 177–184.

    CAS  PubMed  Google Scholar 

  127. Harris, M., 1973, Phenotypic expression of drug resistance in hybrid cells, J. Nat. Cancer Inst. 50: 423–429.

    CAS  PubMed  Google Scholar 

  128. Hasmi, S., May, S. R., Krooth, R. S., and Miller, O. J., 1975, Concurrent development of resistance to 6-azauridine and adenosine in a mouse cell line, J. Cell. • Physiol. 86: 191–200.

    Google Scholar 

  129. Hatzfeld, J., and Buttin, G., 1975, Temperature-sensitive cell cycle mutants of Chinese hamster cell line with a reversible block in cytokinesis, Cell 5: 123–129.

    CAS  PubMed  Google Scholar 

  130. Henneberry, R. C., Fishman, P. H., and Freese, E., 1975, Morphological changes in cultured mammalian cells: Prevention by the calcium inophore A23187. Cell 5: 1–9.

    CAS  PubMed  Google Scholar 

  131. Hochman, J., Insel, P. A., Boume, H. R., Coffino, P., and Tomkins, G. M., 1975, A structural gene mutation affecting the regulatory subunit of cyclic AMP-dependent protein kinase in mouse lymphoma cells. Proc. Natl. Acad. Sci. U.S.A. 72: 5051–5055.

    CAS  PubMed Central  PubMed  Google Scholar 

  132. Hohmann, L. K., and Barnhart, B. J., 1975, A modified replicate plating technique for use in somatic cell mutant selection, J. Cell. Biol. 67: 176a.

    Google Scholar 

  133. Horst, J., Kluge, F., Beyreuther, K., and Gerok, W., 1975, Gene transfer to human cells: Transducing phage Xp lac gene expression in GM,-gangliosidosis fibroblasts, Proc. Natl. Acad. Sci. U.S.A. 72: 3531–3535.

    CAS  PubMed Central  PubMed  Google Scholar 

  134. Hsie, A. W., Brimer, P. A., Mitchell, T. J., and Gosslee, D. G., 1975, The dose—response relationship for ethyl methanesulfonate-induced mutations at the hypoxanthine-guanine phosphoribosyltransferase locus in Chinese hamster ovary cells, Somatic Cell Genet. 1: 247–261.

    CAS  PubMed  Google Scholar 

  135. Hsie, A. W., Brimer, P. A., Mitchell, T. J., and Gosslee, D. G., 1975, The dose—response relationship for ultraviolet-light-induced mutations at the hypoxanthineguanine phosphoribosyltransferase locus in Chinese hamster cells, Somatic Cell Genet. 1: 383–389.

    CAS  PubMed  Google Scholar 

  136. Hsie, A. W., Jones, C., and Puck, T. T., 1971, Further changes in differentiation state accompanying the conversion of Chinese hamster cells to fibroblast form by dibutyrl adenosine cyclic 3’:5’-monophosphate and hormones, Proc. Natl. Acad. Sci. U.S.A. 68: 1648–1652.

    CAS  PubMed Central  PubMed  Google Scholar 

  137. Hsie, A. W., and Puck, T. T., 1971, Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3’:5’-monophosphate and testosterone, Proc. Natl. Acad. Sci. U.S.A. 68: 358–361.

    CAS  PubMed Central  PubMed  Google Scholar 

  138. Huberman, E., Donovan, P. J., and DiPaolo, J. A., 1972, Mutation and transformation of cultured mammalian cells by N-acetoxy-N-2-fluorenylacetamide, J. Nat. Cancer Inst. 48: 837–840.

    CAS  PubMed  Google Scholar 

  139. Huberman, E., and Heidelberger, C., 1972, The mutagenicity to mammalian cells of pyrimidine nucleoside analogs, Mutat. Res. 14: 130–132.

    CAS  PubMed  Google Scholar 

  140. Ishii, K., and Green, H., 1973, Lethality of adenosine for cultured mammalian cells by interference with pyrimidine biosynthesis, J. Cell Sci. 13: 429–439.

    CAS  PubMed  Google Scholar 

  141. Ittensohn, O. L., and Hutchinson, D. J., 1969, Cytologic manifestations of phagocytosis of L1210 chromosomes by LI210 cells in culture, Exp. Cell Res. 55: 149–154.

    CAS  PubMed  Google Scholar 

  142. Jiménez de Astia, L., Surian, E. S., Flawia, M. M., and Torres, H. N., 1973, Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts, Proc. Natl. Acad. Sci. U.S.A. 70: 1388–1392.

    Google Scholar 

  143. Johnson, G. S., D’Armiento, M., and Carchman, R. A., 1974, N6-substituted adenines induce cell elongation irrespective of the intra-cellular cyclic AMP levels, Exp. Cell Res. 85: 47–56.

    CAS  PubMed  Google Scholar 

  144. Johnson, G. S., Friedman, R. M., and Pastan, I., 1971, Restoration of several morphological characteristics of normal fibroblasts in sacoma cells treated with adenosine 3’:5’-cyclic monophosphate and its derivatives. Proc. Natl. Acad. Sci. U.S.A. 68: 425–429.

    CAS  PubMed Central  PubMed  Google Scholar 

  145. Johnson, G. S., and Pastan, I., 1972, Cyclic AMP increases the adhesion of fibroblasts to substratum, Nature (London) New Biol. 236: 247–249.

    CAS  Google Scholar 

  146. Johnson, R. T., and Rao, P. N., 1970, Mammalian cell fusion: Induction of premature chromosome condensation in interphase nuclei, Nature 226: 717–722.

    CAS  PubMed  Google Scholar 

  147. Jones, C., Wuthier, P., and Puck, T. T., 1975. Genetics of somatic cell surface antigens. III. Further analysis of the A,, marker, Somatic Cell Genet. 1: 235–246.

    CAS  PubMed  Google Scholar 

  148. Kao, F.-T., Johnson, R. T., and Puck, T. T., 1969, Complementation analysis on virus-fused Chinese hamster cells with nutritional markers, Science 164: 312–314.

    CAS  PubMed  Google Scholar 

  149. Kao. F.-T., Jones, C., and Puck, T. T., 1976, Genetics of somatic mammalian cells: Genetic, immunologic, and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes, Proc. Natl. Acad. Sci. U.S.A. 73: 193–197.

    Google Scholar 

  150. Kao, F.-T., and Puck, T. T., 1968, Isolation of nutritionally deficient mutants of Chinese hamster cells, in “Proc. 12th Intl. Congr. Genetics.” Vol. 1, p. 157, Science Council of Japan, Tokyo.

    Google Scholar 

  151. Kao, F.-T., and Puck, T. T., 1968, Genetics of somatic mammalian cells. VII. Induction and isolation of nutritional mutants in Chinese hamster cells, Proc. Natl. Acad. Sci. U.S.A. 60: 1275–1281

    CAS  PubMed Central  PubMed  Google Scholar 

  152. Kao, F.-T., and Puck, T. T., 1970, Genetics of somatic mammalian cells: Linkage studies with human—Chinese hamster cell hybrids, Nature 228: 329–332.

    CAS  PubMed  Google Scholar 

  153. Kao, F.-T., and Puck, T. T., 1974, Induction and isolation of auxotrophic mutants in mammalian cells, in “Methods in Cell Biology,” Vol. 8 (D. M. Prescott, ed.), pp. 23–39, Academic Press, New York.

    Google Scholar 

  154. Kato, H., Sekiya, K., and Yosida, T. H., 1971, Uptake of isolated chromosomes by mammalian cells and protective effect of protamine sulfate, Exp. Cell Res. 65: 454–462.

    CAS  PubMed  Google Scholar 

  155. Kaufman, E. R., and Davidson, R. L., 1975, Control of the expression of a herpes simplex virus thymidine kinase gene incorporated into thymidine kinase-deficient mouse cells, Somatic Cell Genet. 1: 153–164.

    CAS  PubMed  Google Scholar 

  156. Kay, E. R. M, 1961, Incorporation of deoxyribonucleic acid by mammalian cells in vitro, Nature (London) 191: 387–388.

    CAS  Google Scholar 

  157. Kelly-Garvert, F., and Legator, M. S., 1973, Cytogenetic and mutagenic effects of DDT and DDE in a Chinese hamster cell line, Mutat. Res. 17: 223–229.

    CAS  PubMed  Google Scholar 

  158. Kislev, N., Spolsky, C. M., and Eisenstadt, J. M.. 1973, Effect of chloramphenicol on the ultra-structure of mitochondria in sensitive and resistant strains of HeLa, J. Cell Biol. 57: 571–579.

    CAS  PubMed Central  PubMed  Google Scholar 

  159. Kit, S., and Dubbs, D. R., 1963, Acquisition of thymidine kinase activity by herpes simplex infected mouse fibroblast cells, Biocl,em. Biophys. Res. Commun. 11: 55–59.

    CAS  Google Scholar 

  160. Klein, G., 1963, Genetics of somatic cells, in “Methodology in Mammalian Genetics” (W. J. Burdette, ed.), pp. 407–468, Holden-Day, San Francisco.

    Google Scholar 

  161. Klinger, H. P., and Shin, S. I., 1974, Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion, Proc. Natl. Acad. Sci. U.S.A. 71: 1398–1402.

    CAS  PubMed Central  PubMed  Google Scholar 

  162. Knapp, A. G. A. C., and Simons, J. W. I. M., 1975, A mutational assay system for L5178Y mouse lymphoma cells, using hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficency as marker. The occurrence of a long expression time for mutations induced by X-rays and EMS, Mutat. Res. 30: 97–110.

    Google Scholar 

  163. Kozak, C, Nichols, E. A., and Ruddle, F. H., 1974, A somatic cell approach to the genetic analysis of the laboratory mouse, J. Exp. Zool. 187: 303–308.

    CAS  PubMed  Google Scholar 

  164. Kozak, C., Nichols, E., and Ruddle, F. H., 1975, Gene linkage analysis in the mouse by somatic cell hybridization: Assignment of adenine phosphoribosyltransferase to chromosome 8 and a-galactosidase to the X chromosome, Somatic Cell Genet. 1: 371–382.

    CAS  PubMed  Google Scholar 

  165. Kraus, L. M., 1961, Formation of different hemoglobins in tissue culture of human bone marrow treated with human deoxyribonucleic acid, Nature (London) 192: 1055 1957.

    Google Scholar 

  166. Krevitsky, T. A., Papaioannou, R., and Elion, G. B., 1969, Human hypoxanthine phosphoribosyltransferase, J. Biol. Chem. 224: 1263–1270.

    Google Scholar 

  167. Krooth, R. S., Darlington, G. A., and Velazquez, A. A., 1968, The genetics of cultured mammalian cells, Annu. Rev. Genet. 2: 141–164.

    Google Scholar 

  168. Kruse, P. F., Jr., and Patterson, M. K., Jr. (eds.), 1973, “Tissue Culture. Methods and Applications,” Academic Press, New York.

    Google Scholar 

  169. Kucherlapati, R. S., Baker, R. M., and Ruddle, F. H., 1975, Ouabain as a selective agent in the isolation of somatic cell hybrids, Cytogenet. Cell Genet. 14: 192–193.

    Google Scholar 

  170. Kucherlapati, R. S., Creagan, R. P., and Ruddle, F. H., 1974, Progress in human gene mapping by somatic cell hybridization, in “The Cell Nucleus,” Vol. 11 (H. Busch, ed.), pp. 209–222, Academic Press, New York.

    Google Scholar 

  171. Kuo, J. F., and Greengard, P., 1969, Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3’,5’-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom, Proc. Natl. Acad. Sci. U.S.A. 64: 1349–1355.

    CAS  PubMed Central  PubMed  Google Scholar 

  172. Kuroki, T., 1973. Colony formation of mammalian cells on agar plates and its aPplication to Lederberg’s replica plating, Exp. Cell Res. 80: 55–62.

    CAS  PubMed  Google Scholar 

  173. Kuroki, T., 1974, Isolation of UV-sensitive clones from mouse cell lines by agar plate culture and replica plating and their possible application in the study of chemical carcinogenesis, in “Chemical Carcinogenesis Assays” (R. Montesano and L. Tomatis, eds.), pp. 147–169, IARC Scientific Publications No. 10, Intl. Agency for Research on Cancer, Lyon.

    Google Scholar 

  174. Kuroki, T., 1975, Agar plate culture and Lederberg style replica plating of mammalian cells, in “Methods in Cell Biology,” Vol. 9 (D. M. Prescott, ed.), pp. 157–178, Academic Press, New York.

    Google Scholar 

  175. Kusano, T., Long, C., and Green, H., 1971, A new reduced human-mouse somatic cell hybrid containing the human gene for adenine phosphoribosyltransferase, Proc. Natl. Acad. Sci. U.S.A. 68: 82–86.

    CAS  PubMed Central  PubMed  Google Scholar 

  176. Langan, T. A., 1973, Protein kinases and protein kinase substrates. in “Advances in Cyclic Nucleotide Research,” Vol. 3 (P. Greengard and G. A. Robinson, eds.), pp. 99–153, Raven Press, New York.

    Google Scholar 

  177. Lederberg, J., and Lederberg, E. M., 1952, Replica plating and indirect selection of bacterial mutants, J. Bacteriol. 63: 399–406.

    CAS  PubMed Central  PubMed  Google Scholar 

  178. Lederberg, J., and Zinder, N., 1948, Concentration of biochemical mutants of bacteria with penicillin, J. Am. Chem. Soc. 70: 4267–4268.

    CAS  PubMed  Google Scholar 

  179. Lieberman, I., and Ove, P., 1960, Enzyme studies with mutant mammalian cells, J. Biol. Chem. 235: 1765–1768.

    CAS  PubMed  Google Scholar 

  180. Linnane, A. W., Haslam, J. M., Lukins, H. B., and Nagley. P., 1972, The biogenesis of mitochondria in microorganisms, Anna. Rev. Microbial. 26: 163–198.

    CAS  Google Scholar 

  181. Linnane, A. W., Saunders, G. W., Gingold, E. B., and Lukins, H. B., 1968, The biogenesis of mitochondria. V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. U.S.A. 59: 903–910.

    CAS  Google Scholar 

  182. Liskay, R. M., 1973, A study of cell cycle variants in an established mouse line, J. Cell Biol. 59: 197a.

    Google Scholar 

  183. Littlefield, J. W., 1964, Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants, Science 145: 709–710.

    CAS  PubMed  Google Scholar 

  184. Lubin, M, 1959, Selection of auxotrophic bacterial mutants by tritium-labeled thymidine, Science 129: 838–839.

    CAS  PubMed  Google Scholar 

  185. Lubin, M. J., 1962, Enrichment of auxotrophic mutant populations by recycling, J. Bacteriol. 83: 696–697.

    CAS  PubMed Central  PubMed  Google Scholar 

  186. Lwoff, A., Dulbecco, R., Vogt, M., and Lwoff, M., 1955, Kinetics of the release of poliomyelitis virus from single cells, Virology 1: 128–139.

    CAS  PubMed  Google Scholar 

  187. Macpherson, I., and Bryden, A., 1971, Mitomycin C treated cells as feeders, Exp. Cell Res. 69: 240–241.

    CAS  PubMed  Google Scholar 

  188. McBride, O. W., and Ozer, H. L., 1973, Transfer of genetic information by purified metaphase chromosomes, Proc. Natl. Acad. Sci. U.S.A. 70: 1258–1262.

    CAS  PubMed Central  PubMed  Google Scholar 

  189. McDougall, J. K., Kucherlapati, R., and Ruddle, F. H., 1973, Localization and induction of the hyman thymidine kinase gene by adenovirus 12, Nature (London) New Biol. 245: 172–175.

    CAS  Google Scholar 

  190. McKusick, V. A., and Chase, G. A., 1973, Human genetics. Anno. Rev. Genet. 7: 435–473.

    CAS  Google Scholar 

  191. Marin, G., 1969, Selection of chromosomal segregants in a “hybrid” line of Syrian hamster fibroblasts, Exp. Cell Res. 57: 29–36.

    CAS  PubMed  Google Scholar 

  192. Martin, G. M., and Tuan, A., 1966, A definitive cloning technique for human fibroblast cultures, Proc. Soc. Exp. Biol. Med. 123: 138–140.

    CAS  PubMed  Google Scholar 

  193. Mathias, A., and Fischer, G. A., 1962, The metabolism of thymidine by murine leukemic lymphoblasts (L5178Y), Biochem. Pharmacol. 11: 57–68.

    CAS  PubMed  Google Scholar 

  194. Medrano, L., and Green, H., 1974, A uridine kinase-deficient mutant of 3T3 and a selective method for cells containing the enzyme, Cell 1: 23–26.

    CAS  Google Scholar 

  195. Meiss, H. K., and Basilico, C., 1972, Temperature sensitive mutants of BHK 21 cells, Nature (London) New Biol. 239: 66–68.

    CAS  Google Scholar 

  196. Merril, C. R., Geier, M. R., and Petricciani, J. C., 1971, Bacterial virus gene expression in human cells, Nature (London) 233: 398–400.

    CAS  Google Scholar 

  197. Meuth, M., and Green, H., 1974, Alterations leading to increased ribonucleotide reductase in cells selected for resistance to deoxynucleosides, Cell 3: 367–374.

    CAS  PubMed  Google Scholar 

  198. Mezger-Freed, L., 1972, Effect of ploidy and mutagen on bromodeoxyuridine resistance in haploid and diploid frog cells, Nature (London) New Biol. 235: 245–246.

    CAS  Google Scholar 

  199. Migeon, B. R., and Childs, B., 1970, Hybridization of mammalian somatic cells, Prog. Med. Genet. 7: 1–28.

    CAS  PubMed  Google Scholar 

  200. Minna, J. D., and Coon, H. G., 1974, Human x mouse hybrid cells segregating mouse chromosomes and isozymes, Nature (London) 252: 401–404.

    CAS  Google Scholar 

  201. Minna, J. D., Marshall, T. H., and Shaffer-Berman, P. V.. 1975, Chinese hamster x mouse hybrid cells segregating mouse chromosomes and isozymes, Somatic Cell Genet. 1: 355–369.

    CAS  PubMed  Google Scholar 

  202. Mitchell, C. H., England, J. M., and Attardi, G.. 1975, Isolation of chloramphenicolresistant variants from a human cell line, Somatic Cell Genet. 1: 215–234.

    CAS  PubMed  Google Scholar 

  203. Morris, N. R., and Fischer, G. A., 1963, Studies concerning the inhibition of cellular reproduction by deoxyribonucleosides. I. Inhibition of the synthesis of deoxycytidine by a phosphorylated derivative of thymidine. Biochim. Biophvs. Acta 68: 84–92.

    CAS  Google Scholar 

  204. Morris, N. R., Reichard, P., and Fischer, G. A., 1963, Studies concerning the inhibition of cellular reproduction by deoxyribonucleosides. II. Inhibition of the synthesis of deoxycytidine by thymidine, deoxyadenosine and deoxyguanosine, Biochim. Biophys. Acta 68: 93–99.

    CAS  Google Scholar 

  205. Morrissey, A. T. E., and Fraenkel, D. G., 1968, Selection of fructose-6-phosphate kinase mutants in Escherichia coli, Biochem. Biophys. Res. Commun. 32: 467–473.

    CAS  Google Scholar 

  206. Mortimer, R. K., and Manney, T. R., 1971, Mutation induction in yeast, in “Chemical Mutagens,” Vol. 1 (A. Hollaender, ed.), pp. 289–310, Plenum Press, New York.

    Google Scholar 

  207. Munyon, W., Kraiselburd, E., Davis, D., and Mann, J., 1971, Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus, J. Virol. 7: 813–820.

    CAS  PubMed Central  PubMed  Google Scholar 

  208. Myhr, B. C., and DiPaolo, J. A., 1975, Requirement for cell dispersion prior to selection of induced azaguanine-resistant colonies of Chinese hamster cells, Genetics 80: 157–169.

    CAS  PubMed Central  PubMed  Google Scholar 

  209. Nabholz, M., Miggiano, V., and Bodmer, W., 1969, Genetic analysis with human-mouse somatic cell hybrids, Nature (London) 223: 358–363.

    CAS  Google Scholar 

  210. Naha, P. M., 1969, Temperature sensitive conditional mutants of monkey kidney cells, Nature (London) 223: 1380–1381.

    CAS  Google Scholar 

  211. Naha, P. M., 1974, Isolation of temperature-sensitive mutants of mammalian cells, in “Methods in Cell Biology,” Vol. 8 (D. M. Prescott, ed.), pp. 41–46, Academic Press, New York.

    Google Scholar 

  212. Ohno, S., 1969, Evolution of sex chromosomes in mammals, Annz. Rev. Genet. 3: 495–524.

    Google Scholar 

  213. Pastan, I., and Perlam, R., 1970, Cyclic adenosine monophosphate in bacteria, Science 169: 339–344.

    CAS  PubMed  Google Scholar 

  214. Patterson, D., Kao, F.-T., and Puck, T. T., 1974, Genetics of somatic mammalian cells: Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism, Proc. Natl. Acad. Sci. U.S.A. 71: 2057–2061.

    CAS  PubMed Central  PubMed  Google Scholar 

  215. Perlman, R., and Pastan, I., 1969, Pleiotropic deficiency of carbohydrate utilization in an adenylcyclase deficient mutant of Escherichia coli, Biochem. Biophys. Res. Commun. 37: 151–157.

    CAS  Google Scholar 

  216. Petricciani, J. C., and Patterson, R. M., 1974, Incorporation of exogeneous DNA into mammalian chromosomes, Nature (London) 249: 640–650.

    Google Scholar 

  217. Phillips, S. G., and Phillips, D. M., 1969, Sites of nucleolus production in cultured Chinese hamster cells, J. Cell Biol. 40: 248–268.

    CAS  PubMed Central  PubMed  Google Scholar 

  218. Pious, D., Hawley, P., and Forrest, G., 1973, Isolation and characterization of HL-A variants in cultured human lymphoid cells, Proc. Natl. Acad. Sci. U.S.A. 70: 1397–1400.

    CAS  PubMed Central  PubMed  Google Scholar 

  219. Pontecorvo, G., 1971, Induction of directional chromosomal elimination in somatic cell hybrids, Nature (London) 230: 367–369.

    CAS  Google Scholar 

  220. Pontecorvo, G., 1974, Induced chromosome elimination in hybrid cells, in “Somatic Cell Hybridization” (R. L. Davidson and F. de la Cruz, eds.), pp. 65–69, Raven Press, New York.

    Google Scholar 

  221. Prescott, D. M., Myerson, D., and Wallace, J.,’ 1972. Enucleation of mammalian cells with cytochalasin B, Exp. Cell Res. 71: 480–485.

    CAS  PubMed  Google Scholar 

  222. Preud’Homme, J.-L, Birshtein, B. K., and Scharff, M. D., 1975, Variants of a mouse myeloma cell line that synthesize immunoglobulin heavy chains having an altered serotype, Proc. Natl. Acad. Sci. U.S.A. 72: 1427–1430.

    PubMed Central  PubMed  Google Scholar 

  223. Puck, T. T., and Kao, F.-T, 1967, Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants, Proc. Natl. Acad. Sci. U.S.A. 58: 1227–1234.

    CAS  Google Scholar 

  224. Puck, T. T., and Marcus, P. I., 1955, A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: The use of X-irradiated cells to supply conditioning factors, Proc. Natl. Acad. Sci. U.S.A. 41: 432–437.

    CAS  PubMed Central  PubMed  Google Scholar 

  225. Puck, T. T., Wuthier, P., Jones, C., and Kao, F.-T., 1971, Genetics of somatic mammalian cells: Lethal antigens as genetic markers for study of human linkage groups. Proc. Natl. Acad. Sci. U.S.A. 68: 3102–3106.

    CAS  PubMed Central  PubMed  Google Scholar 

  226. Qasba, P. K., and Aposhian, H. V., 1971, DNA and gene therapy: Transfer of mouse DNA to human and mouse embryonic cells by polyoma pseudovirions, Proc. Natl. Acad. Sci. U.S.A. 68: 2345–2349.

    CAS  PubMed Central  PubMed  Google Scholar 

  227. Rao, P. N., and Johnson, R. T., 1972, Premature chromosome condensation: A mechanism for the elimination of chromosomes in virus-fused cells, J. Cell Sci. 10: 495–513.

    CAS  PubMed  Google Scholar 

  228. Ricciuti, F. C., and Ruddle, F. H., 1973, Assignment of three gene loci (PGK, HGPRT, G6PD) to the long are of the human X chromosome by somatic cell genetics, Genetics 74: 661–678.

    CAS  PubMed Central  PubMed  Google Scholar 

  229. Robb, J. A., 1970, Microcloning and replica plating of mammalian cells, Science 170: 857–858.

    CAS  PubMed  Google Scholar 

  230. Roberts, C. F., 1959, A replica plating technique for the isolation of nutritionally exacting mutants of a filamentous fungus (Aspergillus nidulans), J. Gen. Microbiol. 20: 540–548.

    CAS  PubMed  Google Scholar 

  231. Robins, A. B., and Taylor, D. M., 1968, Nuclear uptake of exogeneous DNA into mammalian cells in culture, Nature 217: 1228–1231.

    CAS  PubMed  Google Scholar 

  232. Roosa, R. A., and Bailey, E., 1970, DNA mediated transformation of mammalian cells in culture. Increased transforming efficiency following sonication, J. Cell. Physiol. 75: 137–150.

    CAS  PubMed  Google Scholar 

  233. Roscoe, D. H., Read, M., and Robinson, H., 1973, Isolation of temperature sensitive mammalian cells by selective detachment, J. Cell. Physiol. 82: 325–332.

    CAS  PubMed  Google Scholar 

  234. Rosenstraus, M., and Chasin, L. A., 1975, Isolation of mammalian cell mutants deficient in glucose-6-phosphate dehydrogenase activity: Linkage to hypoxanthine phosphoribosyl transferase, Proc. Natl. Acad. Sci. U.S.A. 72: 493–497.

    CAS  PubMed Central  PubMed  Google Scholar 

  235. Roth, J. R., 1974, Frameshift mutations, Anna. Rev. Genet. 8: 319–346.

    CAS  Google Scholar 

  236. Ruddle, F. H., and Creagan, R. P., 1975, Parasexual approaches to the genetics of man, Annu. Rev. Genet. 9: 407–486.

    CAS  PubMed  Google Scholar 

  237. Sanford, K. K., Earle, W. R., and Likely, G. D., 1948, The growth in vitro of single isolated tissue cells, J. Nat. Cancer Inst. 9: 229–246.

    CAS  PubMed  Google Scholar 

  238. Scaletta, L. J., and Ephrussi, B., 1965, Hybridization of normal and neoplastic cells in vitro, Nature (London) 205: 1169.

    Google Scholar 

  239. Scharff, M. D., Birshstein, B., Dharmgrongartama, B., Frank, L., Kelly, T., Kuehl, W. M., Margulies, D., Morrison, S. L., Preud’Homme, J.-L., and Weitzman, S., 1975, The use of mutant myeloma cells to explore the production of immunoglobulins, in “Molecular Approaches to Immunology” (E. E. Smith and D. W. Ribbons, eds.), pp. 109–129, Academic Press, New York.

    Google Scholar 

  240. Scheffler, I. E., 1974, Conditional lethal mutants of Chinese hamster cells: mutants requiring exogeneous carbon dioxide for growth, J. Cell. Physiol. 83: 219–230.

    CAS  PubMed  Google Scholar 

  241. Schenck, D. M., and Moskowitz, M., 1958, Method for isolating single cells and preparation of clones from human bone marrow cultures, Proc. Soc. Exp. Biol. Med. 99: 30–33.

    CAS  PubMed  Google Scholar 

  242. Schwartz, A. G., Cook, P. R., and Harris, H., 1971, Correction of a genetic defect in a mammalian cell, Nature (London) New Biol. 230: 5–8.

    CAS  Google Scholar 

  243. Secher, D. S., Cotton, R. G., and Milstein, C., 1973, Spontaneous mutation in tissue culture-chemical nature of variant immunoglobulin from mutant clones of MOPC 21, FEBS Lett. 37: 311–316.

    CAS  PubMed  Google Scholar 

  244. Sekiguchi, T., Sekiguchi, F., Satake, S., and Yosida, T. H., 1969, Nondegraded incorporation of highly polymerized DNA and isolated metaphase chromosomes into cultured mammalian cells, and replication of the incorporated heterologous chromosomes in the cultured cells, Jpn. J. Med. Sci. Biol. 22: 72–73.

    CAS  PubMed  Google Scholar 

  245. Sekiguchi, T., Sekiguchi, F., and Yamada, M. A., 1973, Incorporation and replication of foreign metaphase chromosomes in cultured mammalian cells. Exp. Cell Res. 80: 223–236.

    CAS  PubMed  Google Scholar 

  246. Sharp, J. D., Capecchi, N. E., and Capecchi, M. R., 1973. Altered enzymes in drug-resistant variants of mammalian tissue culture cells. Proc. Natl. Acad. Sci. U.S.A. 70: 3145–3149.

    CAS  PubMed Central  PubMed  Google Scholar 

  247. Sherman, F., Stewart, J. W., Jackson, M., Gilmore, R. A., and Parker, J. H., 1974, Mutants of yeast defective in iso-l-cytochrome c, Genetics 77: 255–284.

    CAS  Google Scholar 

  248. Sibley, C. H., and Tomkins, G. M., 1974, Isolation of lymphoma cell variants resistant to killing by glucocorticoids, Cell 2: 213–220.

    CAS  PubMed  Google Scholar 

  249. Sibley, C. H., and Tomkins, G. M., 1974, Mechanisms of steroid resistance, Cell 2: 221–227.

    CAS  PubMed  Google Scholar 

  250. Simons, J. W. I. M., 1974, Dose-response relationships for mutants in mammalian somatic cells in vitro, Mutat. Res 25: 219–227.

    CAS  Google Scholar 

  251. Siniscalco, M., Klinger, H. P., Eagle, H., Koprowski, H., Fujimoto, W. Y., and Seegmiller, J. E., 1969, Evidence for intergenic complementation in hybrid cells derived from two human diploid strains each carrying an X-linked mutation. Proc. Natl. Acad. Sci. U.S.A. 62: 793–799.

    CAS  PubMed Central  PubMed  Google Scholar 

  252. Smith, B. J., and Wiggleworth, N. M., 1972, Cell line which is temperature-sensitive for cytokinesis, J. Cell. Physiol. 80: 253–260.

    CAS  PubMed  Google Scholar 

  253. Smith, D. B., and Chu, E. H. Y., 1973, Isolation and characterization of temperature-sensitive mutants in a Chinese hamster cell line, Mutat. Res. 17: 113–120.

    CAS  PubMed  Google Scholar 

  254. Smith, J. D., 1972, Genetics of transfer RNA, Annu. Rev. Genet. 6: 235–256.

    CAS  PubMed  Google Scholar 

  255. Spolsky, C. M., and Eisenstadt, J. M., 1972, Chloramphenicol-resistant mutants of human HeLa cells, FEBS Lett. 25: 319–324.

    CAS  PubMed  Google Scholar 

  256. Stamato, T. D., 1975, A replica plating method for Chinese ovary (CHO) cells using nylon cloth: application to mutant isolation, J. Cell. Biol. 67: 416a.

    Google Scholar 

  257. Stanley, P., Caillibot, V., and Siminovitch, L., 1975. Stable alterations at the cell membrane of Chinese hamster ovary cells resistant to the cytotoxicity of phytohemagglutinin, Somatic Cell Genet. 1: 3–26.

    CAS  PubMed  Google Scholar 

  258. Stark, R. M., and Littlefield, J. W., 1974, Mutagenic effect of BUDR in diploid human fibroblasts, Murat. Res. 22: 281–286.

    CAS  Google Scholar 

  259. Stoker, M. G. P., 1964, A simple marker technique for cells in culture, Exp. Cell Res. 35: 429–431.

    CAS  PubMed  Google Scholar 

  260. Stutts, P., and Brockman, R. W., 1963, A biochemical basis for resistance of L1210 mouse leukemia to 6-thioguanine, Biochem. Pharmacol. 12: 97–104.

    CAS  PubMed  Google Scholar 

  261. Subak-Sharpe, J. H., Bürk, R. R., and Pitts, J. D., 1966. Metabolic co-operation by cell to cell transfer between genetically different mammalian cells in tissue culture, Heredity 21: 342–343.

    Google Scholar 

  262. Subak-Sharpe, H., Bürk, R. R., and Pitts, J. D., 1969, Metabolic cooperation between biochemically marked mammalian cells in tissue culture, J. Cell Sci. 4: 353–367.

    CAS  PubMed  Google Scholar 

  263. Sun, N. C., Chang, C. C., and Chu, E. H. Y., 1974, Chromosome assignment of the human gene for galactose-l-phosphate uridyltransferase, Proc. Natl. Acad. Sci. U.S.A. 71: 404–407.

    CAS  PubMed Central  PubMed  Google Scholar 

  264. Sutherland, E. W., and Rall, T. W., 1957, The properties of an adenine ribonucleotide produced with cellular particles, ATP, Mg’, and epinephrine or glucagon, J. Am. Chem. Soc. 79: 3608.

    CAS  Google Scholar 

  265. Sutherland, E. W., and Rall, T. W., 1958, Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles, J. Biol. Chem. 232: 1077–1091.

    CAS  PubMed  Google Scholar 

  266. Sutton, H. E., and Karp, G. W., Jr., 1970, Somatic variation in human glucose-6phosphate dehydrogenase, Genetics 64: s63.

    Google Scholar 

  267. Suzuki, F., and Horikawa, M., 1973, A replica plating method of cultured mammalian cells, in “Methods in Cell Biology,” Vol. 6 (D. M. Prescott, ed.), pp. 127–142, Academic Press, New York.

    Google Scholar 

  268. Suzuki, F., Kashimoto, M., and Horikawa. M., 1972, A replica plating method of cultured mammalian cells for somatic cell genetics, Exp. Cell. Res. 68: 476–479.

    Google Scholar 

  269. Szybalska, E. H., and Szybalski, W., 1962, Genetics of human cell lines. IV. DNA-mediated heritable transformation of a biochemical trait, Proc. Natl. Acad. Sci. U.S.A. 48: 2026–2034.

    CAS  PubMed Central  PubMed  Google Scholar 

  270. Szybalski, W., Szybalska, E. H., and Ragni, G.. 1962, Genetic studies with human cell lines, Nat. Cancer Inst. Monogr. 7: 75–89.

    Google Scholar 

  271. Terasima, T., and Tolmach, L. J., 1963, Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells, Exp. Cell Res. 30: 344–362.

    CAS  PubMed  Google Scholar 

  272. Thirion, J. J., Banville, D., and Noël, H., 1975, Chinese hamster somatic cell mutants resistant to 2-deoxygalactose, Genetics 80: s79-s80.

    Google Scholar 

  273. Thompson, L. H., and Baker, R. M., 1973. Isolation of mutants of cultured mammalian cells, in “Methods in Cell Biology,” Vol. 6 (D. M. Prescott, ed.), pp. 209–281, Academic Press, New York.

    Google Scholar 

  274. Thompson, L. H., Harkins, J. L., and Stanner, C. P., 1973, A mammalian cell mutant with a temperature sensitive leucyl-transfer RNA synthetase, Proc. Natl. Acad. Sci. U.S.A. 70: 3094–3098.

    CAS  PubMed Central  PubMed  Google Scholar 

  275. Thompson, L. H., Mankovitz, R., Baker, R. M., Till, J. E.. Siminovitch, L., and Whitmore, G. F., 1970, Isolation of temperature-sensitive mutants of L-cells, Proc. Natl. Acad. Sci. U.S.A 66: 377–384.

    CAS  PubMed Central  PubMed  Google Scholar 

  276. Thompson, L. H., Scanners, C. P., and Siminovitch. L., 1975, Selection by [’H]amino acids of CHO-cell mutants with altered leucyl-and asparagyl-transfer RNA synthetases, Somatic Celi Genet. 1: 187–208.

    CAS  Google Scholar 

  277. Tischfield, J. A., and Ruddle, F. H., 1974, Assignment of the gene for adenosine phosphoribosyltransferase to human chromosome 16 by mouse-human somatic cell hybridization, Proc. Natl. Acad. Sci. U.S.A. 71: 4519.

    Google Scholar 

  278. Tocchini-Valentini, G. P., Felicetti, L., and Rinaldi, G. M., 1969, Mutants of Escherichia coli blocked in protein synthesis: Mutants with an altered G factor, Cold Spring Harbor Symp. Quant. Biol. 34: 463–468.

    CAS  PubMed  Google Scholar 

  279. Trembath, M. K., Bunn, C. L., Lukins. H. B.. and Linnane, A. W., 1973, Biogenesis of mitochondria 27. Genetic and biochemical characterization of cytoplasmic and nuclear mutations to spiramycin resistance in Saccharomyces cerevisiae, Mol. Gen. Genet. 121: 35–48.

    CAS  Google Scholar 

  280. Umbarger, H. E., 1971, Metabolite analogs as genetic and biochemical probes, Adv. Genet. 16: 119–140.

    CAS  PubMed  Google Scholar 

  281. van Zeeland, A. A., and Simons, J. W. I. M., 1975, The effect of calf serum on the toxicity of 8-azaguanine, Mutat. Res. 27: 135–138.

    PubMed  Google Scholar 

  282. van Zeeland, A. A., and Simons, J. W. I. M., 1976, Linear dose-response relationships after prolonged expression times in V79 Chinese hamster cells, Mutat. Res. 35: 129–138.

    PubMed  Google Scholar 

  283. Wajntal, A., and DeMars, R., 1967, A tetrazolium method for distinguishing between cultured human fibroblasts having either normal or deficient levels of glucose-6phosphate dehydrogenase, Biochem. Genet. 1: 61–71.

    CAS  PubMed  Google Scholar 

  284. Weisberger, A. S., 1962, Induction of altered globin synthesis in human immature erythrocytes incubated with ribonucleoprotein, Proc. Natl. Acad. Sci. U.S.A. 48: 6880.

    Google Scholar 

  285. Weiss, M. C., and Ephrussi, B., 1966, Studies of interspecific (rat x mouse) somatic hybrids. I. Isolation, growth and evolution of the karyotype, Genetics 54: 1095–1109.

    CAS  PubMed Central  PubMed  Google Scholar 

  286. Weiss, M. C., and Green, H., 1967, Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes, Proc. Natl. Acad. Sci. U.S.A. 58: 1104–1111.

    CAS  PubMed Central  PubMed  Google Scholar 

  287. Werkheiser, R., 1961, Specific binding of A-amino folic acid analogues by folic acid reductase, J. Biol. Chem. 236: 888–893.

    CAS  Google Scholar 

  288. Westerveld, A., Visser, R. P. L. S., Freeke, M. A., and Bootsma, D., 1972, Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine phosphoribosyl transferase, and glucose-6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity. Biochem. Genet. 7: 33–40.

    CAS  PubMed  Google Scholar 

  289. Whang-Peng, J., Tjio, J. H., and Cason, J. C., 1967, Reutilization of presumably degraded radioactive label from a chromosomal fraction of homologous and heterologous cells, Proc. Soc. Exp. Biol. Med. 125: 260–263.

    CAS  PubMed  Google Scholar 

  290. Wild, D., 1974, Serum effect on the yield of chemically induced 8-azaguanineresistant mutants in Chinese hamster cell cultures, Mutat. Res. 25: 229–234.

    CAS  PubMed  Google Scholar 

  291. Willecke, K., Davies, P. J., and Reber, T., Chromosomal gene transfer: properties of a new method for mapping closely linked human genes. Cytogenet. Cell Genet. in press.

    Google Scholar 

  292. Willecke, K., and Ruddle, F. H., 1975, Transfer of the gene for hypoxanthineguanine phosphoribosyltransferase of isolated human metaphase chromosomes into murine L-cells, Cytogent. Cell Genet. 14: 458–461.

    CAS  Google Scholar 

  293. Willecke, K., and Ruddle, F. H., 1975, Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated metaphase chromosomes into mouse L-cells, Proc. Natl. Acad. Sci. U.S.A. 72: 1792–1796.

    CAS  PubMed Central  PubMed  Google Scholar 

  294. Willingham, M. C., Carchman. R. A., and Pastan, I. H., 1973, A mutant 3T3 cell with cyclic AMP metabolism sensitive to temperature change, Proc. Natl. Acad. Sci. U.S.A. 70: 2906–2910.

    CAS  Google Scholar 

  295. Wolfner, M., Yep, D., Rasse-Messenguy, F., and Fink, G. R., 1975, Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae, J. Mol. Biol. 96: 273–290.

    CAS  Google Scholar 

  296. Woodward, V. W., DeZeeuw, J. R., and Srb, A. M., 1954, The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia, followed by filtration and selective plating, Proc. Natl. Acad. Sci. U.S.A. 40: 192–200.

    CAS  PubMed Central  PubMed  Google Scholar 

  297. Wullems, G. J., and Van der Horst, J., 1975, Electrophoretic pattern of hypoxanthine phosphoribosyltransferase produced in Chinese hamster cells following incorporation of human chromosomes, Cytogenet. Cell Genet. 14: 462–463.

    CAS  PubMed  Google Scholar 

  298. Wullems, G. J., Van der Horst, J., and Bootsma, D., 1975, Incorporation of isolated metaphase chromosomes and induction of hypoxanthine phosphoribosyltransferase in Chinese hamster cells, Somatic Cell Genet. 1: 137–152.

    CAS  PubMed  Google Scholar 

  299. Wuthier, P., Jones, C., and Puck, T. T., 1973, Surface antigens of mammalian cells as genetic markers, J. Exp. Med. 138: 229–244.

    CAS  PubMed Central  PubMed  Google Scholar 

  300. Xeros, N., 1962, Deoxyriboside control and synchronization of mitosis, Nature 194: 682–683.

    CAS  PubMed  Google Scholar 

  301. Yep, D., 1975, Triazole-alanine resistance in yeast, Ph. D. Thesis, Cornell University, New York.

    Google Scholar 

  302. Yerganian, G., and Nell, M. B., 1966, Hybridization of dwarf hamster cells by U V-inactivated Sendai virus, Proc. Natl. Acad..Sci. U.S.A. 55: 1066–1073.

    CAS  PubMed Central  PubMed  Google Scholar 

  303. Yoshida, A., Beutler, E., and Motulsky, A. G., 1971, Table of human glucose-6phosphate dehydrogenase variants, Bull. W.H.O. 45: 243–253.

    CAS  PubMed Central  PubMed  Google Scholar 

  304. Yosida, T. H., and Sekiguchi, T., 1968, Metaphase figures of rat chromosomes incorporated into mouse cells, Mol. Gen. Genet. 103: 253–257.

    CAS  PubMed  Google Scholar 

  305. Zepp, H. D., Conover, J. H., Hirschhorn, K., and Hodes. H. L., 1971, Human-mosquito somatic cell hybrids induced by ultraviolet-inactivated Sendai virus, Nature (London) New Biol. 229: 119–121.

    CAS  Google Scholar 

  306. Clements, G. B., 1975, Selection of biochemical variant, in some cases mutant, mammalian cells, Adv. Cancer Res. 21: 273–390.

    CAS  PubMed  Google Scholar 

  307. Siminovitch, L., 1976, On the nature of heritable variation in cultured somatic cells, Cell 7: 1–11.

    CAS  PubMed  Google Scholar 

  308. Roy-Burman, P., 1970, “Analogues of Nucleic Acid Compounds,” Springer-Verlag, New York.

    Google Scholar 

  309. Naylor, S. L., Busby, L. L., and Klebe, R. J., 1976, Biochemical selection for mammalian cells: The essential amino acids, Somatic Cell Genet. 2: 93-I1l.

    CAS  PubMed  Google Scholar 

  310. Thirion, J.-P., Banville, D., and Noël, H., 1976, Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose, Genetics 83: 137–147.

    CAS  PubMed Central  PubMed  Google Scholar 

  311. Fox, M., and Boyle, J. M., 1976, Factors affecting the growth of Chinese hamster cells in HAT selection media, Mutat. Res. 35: 465–478.

    Google Scholar 

  312. Jones, G. E., and Sargent, P. A., 1974, Mutants of cultured Chinese hamster cells deficient in adenine phosphoribosyl transferase, Cell 2: 43–54.

    CAS  PubMed  Google Scholar 

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  1. Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan, USA

    Ernest H. Y. Chu & Sandra S. Powell

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  1. Ernest H. Y. Chu
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  1. University College London, London, England

    Harry Harris (Galton Professor of Human Genetics) (Galton Professor of Human Genetics)

  2. Mount Sinai School of Medicine of The City University of New York, USA

    Kurt Hirschhorn (Arthur J. and Nellie Z. Cohen Professor of Genetics and Pediatrics) (Arthur J. and Nellie Z. Cohen Professor of Genetics and Pediatrics)

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Chu, E.H.Y., Powell, S.S. (1976). Selective Systems in Somatic Cell Genetics. In: Harris, H., Hirschhorn, K. (eds) Advances in Human Genetics. Advances in Human Genetics, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0659-8_5

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