Abstract
Biological nitrogen fixation is an extremely energy intensive process. Under ideal conditions the reduction of one molecule of dinitrogen (N2), catalysed by the nitrogenase enzyme complex, requires 16 molecules of ATP. However, in vivo ATP requirements of up to 42 per N2 reduced have been measured (80; see 66). It is therefore not surprising that free-living nitrogen-fixing bacteria, such as Klebsiella pneumoniae, only reduce N2 when nitrogen (N-) starved and tightly regulate their nitrogen-fixation (nif) genes in response to the intracellular concentration of combined nitrogen (N-regulation). In the case of symbiotic nitrogen fixing bacteria such as rhizobia, the situation is different. As docile endosymbionts in nodules, having differentiated into a clearly different physiological state (bacteroids), they are provided by the plant host with (ample) carbon (C-) source(s) for the production of ATP and reducing equivalents, to facilitate nitrogen fixation for the plant’s benefit (see 66). Therefore, the nif/fix genes of these bacteria may not need to be subject to N-regulation.
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References
Albright, L.M., Huala, E. & Ausubel, F.M. (1989) Ann. Rev. Genet. 23, 311–336.
Austin, S., Henderson, N. & Dixon, R. (1990) Eur. J. Biochem. 187, 353–360.
Batut, J., Daveran-Mingot, M.-L., David, M., Jacobs, J., Garnerone, A.M. & Kahn, D. (1989) EMBO J. 8, 1279–1286.
Beynon, J.L., Williams, M.K. & Cannon, F.C. (1988) EMBO J. 7, 7–14.
de Bruijn, F.J. (1989) in Plant-Microbe Interactions, eds. Kosuge, T. & Nester, E.W. (Macmillan, New York), pp. 457–493.
de Bruijn, F.J. & Ausubel, F.M. (1983) Mol. Gen. Genet. 192, 342–353.
de Bruijn, F.J., S. Rossbach & Schell, J. (1985) in Nitrogen Fixation Research Progress, eds. Evans, H.J., Bottomley, P.J. & Newton, W.E. (Martinus Nijhoff, The Hague), p. 218.
de Bruijn, F.J., Pawlowski, K., Ratet, P., Hilgert, U. & Schell, J. (1987) in Molecular Genetics of Plant-Microbe Interactions, eds. Verma, D.P.S. & Brisson, N. (Martinus Nijhoff, The Hague), pp. 266–271.
de Bruijn, F.J., Pawlowski, K., Ratet, P., Hilgert, U., Wong, C.H., Meyer Z.A. H. & Schell, J. (1988) in Nitrogen Fixation: Hundred Years After, eds. Bothe, H. & de Bruijn, F.J. (Fischer, Stuttgart), pp. 351–355.
de Bruijn, F.J., Rossbach, S., Schneider, M., Ratet, P., Messmer, S., Szeto, W.W., Ausubel, F.M. & Schell, J. (1989) J. Bacteriol. 171, 1673–1682.
Buchanan-Wollaston, V., Cannon, M.C. & Cannon, F.C. (1981) Mol. Gen. Genet. 184. 102–106.
Buck, M. (1986) J. Bacteriol. 166, 545–551.
Buck, M., Miller, S., Drummond, M. & Dixon R. (1986) Nature 320, 374–378.
Buck, M., Woodcock, J., Cannon, W., Michenall, L. & Drummond, M. (1987) Mol. Gen. Genet. 210, 140–144.
Carlson, T.A. & Chelm, B.K. (1986) Nature 322, 568–570.
Carlson, T.A., Martin, G.B. & Chelm, B.K. (1987) J. Bacteriol. 169, 5861–5886.
Castano, I., Bastarrachea, F. & Covarrubias, A. (1988) J. Bacteriol. 170, 821–827.
Collins, J.J., Roberts, G.P. & Brill, W.J. (1986) J. Bacteriol. 168, 173–178.
Colonna-Romano, S., Riccio, A., Guida, M., Defez, R., Lamberti, A., laccarino, M., Arnold, W., Priefer, U. & Punier, A. (1987) Nucl. Acids Res. 15, 1951–1964.
Colonna-Romano, S., Arnold, W., Schlüter, A., Boistard, P., Pünier, A. & Priefer, U. (1990) Mol. Gen. Genet., in press.
Contreras, A. & Drummond, M. (1988) Nucl. Acids Res. 16, 4025–4039.
Darrow, R.A. & Knotts, R.R. (1977) Biochem. Biophys. Res. Commun. 78, 554–559.
Darrow, R.A., Christ, D., Evans, W.R., Jones, B.L., Keister, D.L. & Knotts, R.R. (1981) in Current Perspectives in Nitrogen Fixation, eds. Gibson, A.H. & Newton, W.E. (Australian Academy of Sciences, Canberra), pp. 182–185.
David, M., Daveran, M.-L., Batut, J., Dedieu, A., Domergue, O., Ghai, J., Hertig, C., Boistard, P. & Kahn, D. (1988) Cell 54, 671–683.
Dimri, G.P. & Das, K.H. (1988) Mol. Gen. Genet. 212, 360–363.
Ditta, G., Stanfield, S., Corbin, D. & Helinski, D.R. (1980) Proc. Natl. Acad. Sci. USA 77, 7347–7351.
Dixon, R.A. (1988) in The Nitrogen and Sulphur Cycles, eds. Cole, J.A. & Ferguson, S.J. (Cambridge University Press, Cambridge).
Dixon, R.A., Henderson, N.C. & Austin, S. (1988) Nucl. Acids Res. 16, 9933–9946.
Donald, R.G.K. & Ludwig, R.A. (1984) J. Bacteriol. 158, 1144–1151.
Donald, R.G.K., Lapointe, J. & Ludwig, R.A. (1988) J. Bacteriol. 170. 1197–1204.
Dreyfus, B., Garcia, J.L. & Gillis, M. (1988) Int. J. System. Bacteriol. 38, 89–98.
Drummond, M., Whitty, P. & Wootton, J. (1986) EMBO J. 5, 441–447.
Fischer, H.-M., Bruderer, T. & Hennecke, H. (1988) Nucl. Acids Res. 16, 2207–2224.
Fischer, H.-M., Fritsche, S., Herzog, B. & Hennecke, H. (1989) FEBS Lett. 255, 167–171.
Forde, B.G. & Cullimore, J.V. (1989) Oxford Surveys on Plant Molecular and Cell Biology 6, 247–296.
Fuchs, R.L. & Keister, D.L. (1980) J. Bacteriol. 144, 641–648.
Gosink, M.M., Franklin, N.M. & Roberts, G.P. (1990) J. Bacteriol. 172, 1441–1447.
Griffiths, L. & Cole, J.A. (1987) Arch. Microbiol. 147, 364–369.
Gussin, G.N., Ronson, C.W. & Ausubel, F.M. (1986) Ann. Rev. Genet. 20, 567–591.
Henderson, N., Austin, S. & Dixon, R. (1989) Mol. Gen. Genet. 216, 484–491.
Hertig, C., Li, R.Y., Louarn, A.-M., Garnerone, A.-M., David, M., Batut, J., Kahn, D. & Boistard, P. (1989) J. Bacteriol. 171, 1736–1738.
Hilgert, U., Schell, J. & de Bruijn, F.J. (1987) Mol. Gen. Genet. 210, 195–202.
Hill, S. (1988) FEMS Microbiol. Rev. 54, 111–130.
Holtel, A. & Merrick, M. (1989) Mol. Gen. Genet. 217, 474–480.
Holtel, A., Colonna-Romano, S., Guida, M., Riccio, A., Merrick, M.J. & laccarino, M. (1989) FEMS Microbiol. Lett. 58, 203–208.
Jayaraman, P.S., Peakman, T.C., Busby, S.J.W., Quincey, R.V. & Cole, J.A. (1987) J. Mol. Biol. 196, 781–788.
Kahn, M.L., Kraus, J. & Somerville, J.E. (1985) in Nitrogen Fixation Research Progress, eds. Evans, H.J., Bottomley, P.J. & Newton, W.E. (Martinus Nijhoff, Boston), pp. 193–195.
Kondorosi, A., Svab, Z., Kiss, G.B. & Dixon, R.A. (1977) Mol. Gen. Genet. 151, 221–226.
Kullik, I., Hennecke, H. and Fischer, H.-M. (1989) Arch. Microbiol. 151, 191–197.
Kumar, P.S. & Rao, S.L.N. (1986) Curr. Microbiol. 14, 221–226.
Kranz, R.G. & Haselkorn, R. (1986) Proc. Natl. Acad. Sci. USA 83, 6805–6809.
Kustu, S., Santero, E., Keener, J., Popham, D. & Weiss, D. (1988) Microbiol. Rev. 53, 367–376.
Lewis, T.A., Gonzalez, R. & Botsford, J.L (1990) J. Bacteriol. 172, 2413–2420.
Macaluso, A. & Bender, R.A. (1986) in Abstracts of the American Society for Microbiology. (American Society for Microbiology, Washington), p. 154.
Magasanik, B. (1982) Ann. Rev. Genet. 16, 135–168.
Magasanik, B. (1988) Trends Biochem. Sci. 13, 475–479.
Martin, G.B., Chapman, K.A. & Chelm, B.K. (1988) J. Bacteriol. 170, 5452–5459.
Martin, G.B., Thomashow, M.F. & Chelm, B.K. (1989) J. Bacteriol. 171, 5638–5645.
Merrick, M. (1988) in Nitrogen Fixation: Hundred Years After, eds. Bothe, H. & de Bruijn, F.J. (Fischer, Stuttgart), pp. 293–302.
Merrick, M. (1988) in The Nitrogen and Sulphur Cycles, eds. Cole, J.A. & Ferguson, S.J. (Cambridge University Press, Cambridge).
Merrick, M. & Coppard, J.R. (1989) Mol. Microbiol. 3, 1765–1775.
Miflin, B.J. & Cullimore, J.V. (1984) in Genes involved in Microbe-Plant Interaction, eds. Verma, D.P.S. & Hohn, R. (Springer, Wien), pp. 129–178.
Morett, E. & Buck, M. (1988) Proc. Natl. Acad. Sci. USA 85, 9401–9405.
Morett, E., Cannon, W. & Buck, M. (1988) Nucl Acids Res. 16, 11469–11488.
Nees, D.W., Stein, P.A. & Ludwig, R.A. (1988) Nucl. Acids Res. 16, 9839–9853.
O’Brian, M.R. & Maier, R.J. (1989) Biochim. Biophys. Acta 974, 229–246.
O’Gara, F., Sundaram, M. & Meade, J. (1984) FEMS Microbiol. Lett. 24, 241–245.
Osburne, M.S. & Signer, E.R. (1980) J. Bacteriol. 143, 1234–1240.
Pawlowski, K. (1989) PhD thesis (Cologne University, Cologne).
Pawlowski, K., Ratet, P., Schell, J. & de Bruijn, F.J. (1987) Mol. Gen. Genet. 206, 207–219.
Ratet, P., Pawlowski, K., Schell, J. & de Bruijn, F.J. (1989) Mol. Microbiol. 3, 825–838.
Renalier, M.H., Batut, J., Ghai, J., Terzaghi, B., Gherardi, M., David, M., Garnerone, A.-M., Vasse, J., Truchet, G., Huguet, T. & Boistard, P. (1987) J. Bacteriol. 169, 2231–2238.
Ronson, C.W., Nixon, B.T., Albright, L.M. & Ausubel, F.M. (1987) J. Bacteriol. 169, 2424–2431.
Shatters, R.G. & Kahn, M.L. (1989) J. Mol. Evol. 29, 422–428.
Shaw, D.J. and Guest, J.R. (1982) Nucl. Acids Res. 10, 6119–6130.
Somerville, J.E. & Kahn, M.L. (1983) J. Bacteriol. 156, 168–176.
Somerville, J.E., Shatters, R.G. & Kahn, M.L (1989) J. Bacteriol. 171; 5079–5086.
Spiro, S. & Guest, J.R. (1987a) Mol. Microbiol. 1, 53–58.
Spiro, S. & Guest, J.R. (1987b) J. Gen. Microbiol. 133, 3279–3288.
Stam, H., van Verseveld, H.W. & Stouthamer, A.H. (1986) FEMS Microbiol. Lett. 35, 215–220.
Stanley, J., van Slooten, J., Dowling, D.N., Finan, T. & Broughton, W.J. (1989) Mol. Gen. Genet. 217, 528–532.
Szeto, W.W., Nixon, B.T., Ronson, C.W. & Ausubel, F.M. (1987) J. Bacteriol. 169, 1423–1432.
Thöny, B. & Hennecke, H. (1989) FEMS Microbiol. Rev. 63, 341–358.
Thöny, B., Fischer, H.-M., Anthamatten, D., Bruderer, T. & Hennecke, H. (1987) Nucl. Acids Res. 15, 8479–8499.
Thöny, B., Anthamatten, D. & Hennecke, H. (1989) J. Bacteriol. 171, 4162–4169.
Tsuprun, V.L., Zograf, O.N., Orlova, E.V., Kiselev, N.A., Pushkin, A.V., Shiffelova, G.E., Scolovieva, N.A., Evstigneeva, Z.G. & Kretovich, W.L (1987) Biochim. Biophys. Acta 913. 368–376.
Virts, E.L., Stanfield, S.W., Helinski, D.R. & Drtta, GB.S. (1988) Proc. Natl. Acad. Sci. USA 85, 3062–3065.
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deBruijn, F.J. et al. (1990). Regulation of nitrogen fixation and assimilation genes in the free-living versus symbiotic state. In: Gresshoff, P.M., Roth, L.E., Stacey, G., Newton, W.E. (eds) Nitrogen Fixation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6432-0_4
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