Abstract
The copper binding tripeptide, glycyl-L-histidyl-L-lysine [GHK:Cu(II)] has a plethora of biological effects related to the wound healing process. The presence of iron complexes in damaged tissues is detrimental to wound healing, due to local inflammation, as well as microbial infection mediated by iron. To test if the wound healing properties of GHK:Cu(II) are due to an affect on iron metabolism, we examined the effects of GHK:Cu(II) on iron catalyzed lipid peroxidation. GHK:Cu(II) inhibited lipid peroxidation only if the iron source was ferritin. Whereas GHK:Cu(II) inhibited ferritin iron release it did not exhibit significant Superoxide dismutase-like or ceruloplasmin-like activity. We propose that GHK:Cu(II) binds to the channels of ferritin involved in iron release and physically prevents the release of Fe(II). Thus, a biological effect of GHK:Cu(II), possibly related to wound healing, may be the inhibition of ferritin iron release in damaged tissues, preventing inflammation and microbial infections.
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References
Pickart, L. and Lovejoy, S., Biological activity of human plasma copper-binding growth factor glycyl-L-histidyl-L-lysine, in “Methods of Enzymology” 147, 314–328 (1987).
Maquart, F.X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J.C., and Borel, J.P., Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine Cu(2+). FEBS Lett. 238:343 (1988).
Raju, K., Alessandri, G., and Gullino, P., Characterization of a chemoattractant for endothelium induced by angiogenesis effectors. Cancer Res, 44:1579 (1984) .
Sensenbrenner, M., Jaros, G.G. , Moonen, G., and Mandel, P., Effects of synthetic tripeptide on the differentiation of dissociated cerebral hemisphere nerve cells in culture. Neurobiolocrv 5:207 (1975).
Maquart, F.X., Kalis, B., Pickart, L., Gillery, P., Monboisse, J.C., Salagnac, V., and Borel, J.P., Glycyl histidyl-lysine: A copper binding peptide with wound healing properties. (Abstract) in A Hard Look at Collagen-Related Disorders, European Society for Dermatological Research, Liege, Feb. 16–18, 1989,
Zetter, B.R., Rasmussen, N., and Brown, L., An in vivo assay for chemoattactant activity. Lab. Invest. 53:3 62 (1985).
Weinberg, E.D. Iron withholding: A defense against infection and neoplasia. Physiol. Rev. 64:65 (1984),
Thomas, C.E. and Aust, S.D., Rat liver microsomal NADPH-dependent release of iron from ferritin and lipid peroxidation. J. Free Rad. Biol. Med, 1:293 (1985).
Folch, J., Lees, M., and Sloane-Stanley, G.H., A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:466 (1959).
Buege, J.A., and Aust, S.D., Microsomal lipid peroxidation, .in Methods of Enzymology 52:302 (1978).
Stookey, L.L., Ferrozine -A new spectrophotometric reagent for iron. Anal. Chem. 42:779 (1970).
Theil, E.C. Ferritin: Structure, gene regulation, and cellular function in animals, plants, and microorganisms, in Ann. Rev. Biochem. 56:289 (1987).
Stevens, R.G., Jones, D.Y., Micozzi, M.S., and Taylor, P.R., Body iron stores and the risk of cancer. New England J. Med. 319:1047 (1988).
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© 1990 Plenum Press, New York
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Miller, D.M., DeSilva, D., Pickart, L., Aust, S.D. (1990). Effects of Glycyl-Histidyl-Lysyl Chelated Cu(II) on Ferritin Dependent Lipid Peroxidation. In: Emerit, I., Packer, L., Auclair, C. (eds) Antioxidants in Therapy and Preventive Medicine. Advances in Experimental Medicine and Biology, vol 264. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5730-8_11
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DOI: https://doi.org/10.1007/978-1-4684-5730-8_11
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