Abstract
The mapping of mammalian chromosomes can be approached by two basic strategies: genetic linkage and physical mapping. The precision with which genetic mapping can be applied to a given chromosomal region is directly related to the number of polymorphic markers available in that region and the spacing of those markers within the region. Physical mapping techniques are limited by the sizes of DNA fragments that can be manipulated, and until recently, these limits were in the range of tens of kilobase pairs of DNA. Recent technical innovations promise the potential for manipulating mammalian DNA segments hundreds of kilobase pairs in length. It is clear that the power of both genetic-linkage and physical-isolation techniques would be enhanced by the ability to isolate and characterize chromosomal segments 105 base pairs or greater in length by a direct genetic technique. Previously, we have suggested a strategy based on metaphase-chromosome transfer to address this issue (Housman et al., 1983). For the past several years, our research efforts have been devoted to development of this strategy. The results presented herein bear on the utility of chromosome transfer both for the physical mapping of chromosomes and for the development of DNA-based markers at intervals of a centimorgan or less within these chromosomal segments.
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Housman, D.E., Nelson, D.L. (1986). Use of Metaphase-Chromosome Transfer for Mammalian Gene Mapping. In: Kucherlapati, R. (eds) Gene Transfer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5167-2_4
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DOI: https://doi.org/10.1007/978-1-4684-5167-2_4
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